Metabolismo de carbono y flujos de carbono orgánico disuelto (COD) en comunidades de angiospermas marinas: dependencia del estadío de colonización, eutrofización y factores relacionados con el cambio global

Coastal vegetated communities are among the most productive ecosystems on Earth. Their role in the global carbon cycle and how they cope with global change may be more relevant than previously believed. They export large quantities of matter, both in particulate and dissolved forms to adjacent communities. Dissolved organic carbon (DOC) flux play a central role in the marine carbon cycle as an important driver of primary production for other compartments of the food web. While there has been extensive research on DOC dynamics in the open ocean, the role of coastal ecosystems in the global DOC cycle is still inadequately understood, even though these habitats tend to accumulate large amounts of DOC. Few studies have examined the DOC fluxes by marine macrophytic communities (macroalgae and seagrasses) under in situ approaches to determine their overall contribution in the whole system and their subsequent exchange with adjacent communities. Moreover, coastal vegetated communities, especially those dominated by seagrasses, are currently considered one of the most threatened ecosystems on Earth because of anthropogenic pressures, including nutrient increase and climate change. Thus, the overall objective of this Thesis was to evaluate the carbon metabolism and DOC fluxes in communities dominated by seagrasses and elucidate the effects of human–induced disturbances on the carbon dynamics of the community. The results of this Thesis showed that macrophytic communities are highly autotrophic, with large and variable contributions of their different components of the community, and a DOC source for the plankton community. Increase in nutrients concentration triggered that the communites dominated by the macroalge Caulerpa prolifera and the seagrass Cymodocea nodosa moved from autotrophy to heterotrophy in certain seasons of the year and could increase or decrease the DOC release. The response of seagrass communities when subjected to a pH decrease was complex and showed to be species-specific. The pH decrease triggered a significant increase in gross primary production (GPP) and community respiration (R) in seagrasses, which was translated into sucrose increase in aboveground tissues rather than a higher DOC release. Water temperature was the stressor that had a higher positive effect on carbon metabolism, yielding higher seagrass productivity, growth and DOC release. A direct relationship between productivity increase and larger DOC release was found in communities dominated by seagrasses. In addition, a high correlation between DOC release and both water temperature and current velocity was found. This Thesis demonstrated that climatic change and to some extent nutrient enrichment in coastal areas may not be so detrimental than previously believed at least for temperate seagrasses, and even may benefit the productivity and resistance of some temperate species (e.g. Cymodocea nodosa) in the future. The results of this Thesis underline the high productivity of vegetated coastal ecosystems at a local level, which support new insights in the role of the marine primary production in the ocean C sink and the role of the carbon coastal cycle in the global carbon cycle. Finally, this Thesis underscores that the role of seagrass meadows in the carbon coastal cycle will be more relevant in the near future, as higher C uptake and DOC release may occur under forecasted global change conditions

Loss of POC and DOC on seagrass sediments by hydrodynamics

Coastal development and climate change are sparking growing concern about the vulnerability of the organic carbon (OC) stocks in marine sediments to remineralization, especially in high threaten coastal ecosystems like seagrass meadows. Uncertainties still exist regarding the role played by hydrodynamics, seagrass canopies and sediment properties in OC resuspension and remineralization. A set of laboratory experiments were conducted to assess, for the first time, the mechanisms by which the particulate and dissolved organic carbon (POC and DOC) may be released and remineralized under hydrodynamic conditions (i.e., unidirectional and oscillatory flows) in two eelgrass densities and sediments properties (i.e., grain size and OC content). After a gradually increase in hydrodynamic forces, our results demonstrated that the presence of eelgrass reduced sediment erosion and OC loss in high-density canopies, while low-density canopies promote OC resuspension (on average, 1.8-fold higher than high-density canopies). We also demonstrated that unidirectional and oscillatory flows released similar DOC from surface sediments (on average, 15.5 ± 1.4 and 18.4 ± 1.8 g m−2, respectively), whereas oscillatory flow released significantly more POC than unidirectional flows (from 10.8 ± 1.1 to 32.1 ± 5.6 g m−2 for unidirectional and oscillatory flows, respectively). POC and DOC released was strongly influenced by both seagrass meadow structure (i.e., lower eelgrass density and shoot area) and sediment properties (i.e., lower mud and higher sediment water content). We found that, although >74 % of OC in upper sediments was remineralized within 30 days, a relatively high amount of OC in high-density canopies is recalcitrant, highlighting its potential for the formation of blue carbon deposits. This study highlights the vulnerability of OC deposits in seagrass sediments to resuspension if the meadow is degraded and/or the climate change yield stronger storms, which could potentially weaken the seagrass meadows' service as blue carbon ecosystem in the future.12 página

Dissolved organic carbon leaching from microplastics and bioavailability in coastal ecosystems

The dissolved organic carbon (DOC) leached from two types of microplastics (polyethylene and polypropylene) frequently found in coastal areas were evaluated in situ. Subsequently, the bioavailability of leached DOC was assessed for microbial inocula from different coastal communities (i.e., estuarine and open-coastal waters, river-mouth waters and seagrass beds). Leached DOC was largely biodegradable (as much as 85 %). However, seagrass beds and river-mouth waters exhibited lower DOC utilization efficiency than estuarine and open-coastal waters, probably because of differences in their microbial communities. The labile/recalcitrant ratio of DOC leached from plastic was similar under illuminated and dark conditions, whereas DOC leached from polyethylene, rather than DOC leached from polypropylene, was preferentially used by microbial communities. We estimated that as many as 21,000 metric tons of DOC leached from plastics may be released into ocean annually. Our results support the need to consider the potential impacts of coastal plastic pollution on microbial communities, including consideration of the trophic webs and coastal carbon cycle

Effect of In Situ short–term temperature increase on carbon metabolism and dissolved organic carbon (DOC) fluxes in a community dominated by the seagrass Cymodocea nodosa

Seagrasses form one of the most productive and threatened ecosystems worldwide because of global change and anthropogenic pressures. The frequency of extreme climatic events, such as heat waves, are expected to increase and may drive even more adverse effects than gradual warming. This study explores for the first time the effects of a sudden and temporary increase of temperature in situ on carbon metabolism and dissolved organic carbon (DOC) fluxes in a community dominated by a seagrass (Cymodocea nodosa) during two contrasting seasons (winter and summer). Results showed a positive correlation between temperature and seagrass production between seasons, while the experimental sudden and temporary increase in water temperature did not produce significant differences in carbon community metabolism and DOC fluxes in winter. In contrast, high temperature conditions in summer enhanced significantly the net community production and affected positively to DOC fluxes. Hence, this study indicates that a sudden and temporary increase in water temperature, which characterize marine heat waves, in temperate areas may enhance the autotrophic metabolism of seagrass communities and can yield an increase in the DOC released, in contrast to previous researches suggesting solely negative effects on seagrasses

Leaf Senescence of the Seagrass Cymodocea nodosa in Cádiz Bay, Southern Spain

Leaf decay in seagrasses is enhanced in some seasons since large green senescent beach-cast seagrass leaves are frequently recorded during autumn and winter seasons. Here, we explore if senescence is operating in seagrass leaf decay or if hydrodynamic stress is responsible for the seasonal leaf abscission. A seasonal study on the temperate seagrass Cymodocea nodosa was carried out in four locations with contrasting hydrodynamic regimes. The morphological, biomechanical and material properties of C. nodosa were measured. The force required to break the ligule was always lower than that required to break the blade. This could be considered an adaptive strategy to reduce acute drag forces and thus lessen the chance of plant uprooting. The absolute force needed to dislodge the blade at the ligule level varied with season and location, with the lowest forces recorded in autumn. This may indicate that senescence is operating in this species. On the other hand, the minimum estimated failure velocities for leaf abscission were also recorded in autumn. Consequently, this may cause the premature shedding of leaves in this season before the senescence process has finished and can probably explain the occurrence of green beach-cast seagrass leaves usually found during autumn and winter.20 página

Changes in carbon metabolism and dissolved organic carbon fluxes on seagrass patches (Halodule wrightii) with different ages in Southern Gulf of California

Seagrass meadows are highly dynamic, particularly in sites where extreme climatological events may produce a mosaic of patches with different ages. This study evaluates the community carbon metabolism, dissolved organic carbon (DOC) fluxes and associated fauna in patches of Halodule wrightii with different ages since establishment. Net community production declined as patch age increased, probably due to the increase in non-photosynthetic tissues, higher respiration rates of the community assemblage and a likely increase in self-shading of the canopy. The export of DOC was significantly higher in the youngest patches, mainly as a consequence of the lower seagrass net production recorded in older meadows. We concluded that ‘colonizers’ seagrass species may show higher production rates and DOC release during the first stages of colonization, which suggest that, the production, organic carbon exportation and their role as relevant blue carbon communities may be higher than expecte

Effects of a chronic impact on Cymodocea nodosa community carbon metabolism and dissolved organic carbon fluxes

Seagrass communities have been degraded worldwide experiencing elevated shoot density reduction by anthropogenic chronic pressures. This study aims to assess how a chronic (i.e., low intensity but long-lasting) impact that promotes reduced shoot density in a temperate seagrass population may affect community components and functioning. To this end, shoot density was reduced (0, 40, and 75 %) for three months in contrasting seasons (winter and summer), and assessed its effects on biotic components (i.e., seagrasses, macroalgae, macrofauna, and microphytobenthos), as well as on community carbon metabolism, dissolved organic carbon (DOC) fluxes and sediment organic matter (OM) content. Lower shoot densities enhanced the presence of macroalgae and microphytobenthos in the community, while macrofauna remained unchanged. Net community production was significantly reduced with the simulated reduction in shoot density in both seasons (up to 10-fold lower), which shifted the community in winter from being largely autotrophic (CO2 sink) to heterotrophic (CO2 source). This was due to the expected reduction in gross primary production, but also to the unexpected increase in community respiration (up to 2.2-fold higher). Since OM in the sediment was reduced in the simulated shoot density reduction treatments, the increase in sediment bacterial activity may help explain the increase in community respiration. DOC fluxes were also greatly reduced in both seasons (up to 5.5-fold lower), which coupled with the reduced net community production and loss of OM in the sediment may have a continued silent effect on blue carbon capture and storage capacity in this chronically stressed community. This study therefore highlights the importance of chronic impacts that promote the degradation of seagrass communities that may reduce their ability to provide highly valuable ecological services, including the ability to cope with the effects of climate change

Patch age alters seagrass response mechanisms to herbivory damage

Natural disturbances can produce a mosaic of seagrass patches of different ages, which may affect the response to herbivory. These pressures can have consequences for plant performance. To assess how seagrass patch age affects the response to herbivory, we simulated the effect of herbivory by clipping leaves of Halodule wrightii in patches of 2, 4 and 6 years. All clipped plants showed ability to compensate herbivory by increasing leaf growth rate (on average 4.5-fold). The oldest patches showed resistance response by increasing phenolic compounds (1.2-fold). Contrastingly, the concentration of phenolics decreased in the youngest patches (0.26-fold), although they had a similar leaf carbon content to controls. These results suggest that younger plants facing herbivory pressure reallocate their phenolic compounds towards primary metabolism. Results confirm the H. wrightii tolerance to herbivory damage and provides evidence of age-dependent compensatory responses, which may have consequences for seagrass colonization and growth in perturbed habitats

Differential ecophysiological responses to inorganic nitrogen sources (ammonium versus nitrate) and light levels in the seagrass Zostera noltei

eagrasses can use both ammonium (NH4+) and nitrate (NO3−) as inorganic nitrogen (N) sources. However, NO3− uptake and assimilation are energetically more expensive and tightly regulated than NH4+ uptake. The objective of this study was to test the complex interactive effects between different forms of N enrichment (NH4+ and NO3−) and light levels on the morphological and physiological traits in the intertidal seagrass Zostera noltei. Plants were cultured over 40 d under 2 levels of light (low and high) with 2 inorganic N concentrations supplied at the same dose, NO3− (25 μM) and NH4+ (25 μM), and a control, following a 2-factorial design. Results showed a differential response in Z. noltei depending on the inorganic N source and light dose. NH4+ enrichment negatively affected almost all morphometric and dynamic variables analyzed, both in isolation and combined with low light conditions. In contrast, NO3− enrichment had a positive effect on Z. noltei survival compared with the control treatment in terms of net growth rate and rhizomatic growth, mainly under high light conditions. Therefore, our study demonstrated that the effects promoted by nutrient enrichment largely depend on the source of N used. Light levels play a crucial role in this response by potentially shifting the effects from toxic (under low light) to beneficial (under high light) when NO3− is the main N source. Our findings highlight that N form in eutrophication events should be considered when evaluating the potential impacts of nutrient enrichment and light reduction on seagrass communities14 página

Resistance and recovery of benthic marine macrophyte communities to light reduction: Insights from carbon metabolism and dissolved organic carbon (DOC) fluxes, and implications for resilience.

A crucial factor in the long-term survival of benthic macrophyte communities under light-reduction stress is how they balance carbon metabolism during photosynthesis and respiration. In turn, the dissolved organic carbon (DOC) released by these communities, which can be highly light-dependent, stands as a source of carbon, fuelling marine communities and playing an important role in the ocean carbon sequestration. This is the first study to evaluate light-reduction stress and recovery in the seagrass Zostera noltei and the macroalga Caulerpa prolifera. Light reduction led to a significant decrease in the production of both communities from autotrophic to heterotrophic. Results indicated that most of the DOC released by vegetated coastal communities comes from photosynthetic activity, and that the net DOC fluxes can be greatly affected by shading events. Finally, both communities showed resilience underpinned by high recovery but low resistance capacity, with C. prolifera showing the highest resilience to unfavourable light conditions