20 research outputs found
Implementació d’aplicacions web interactives per a l’aprenentatge de conceptes complexos (Informe)
L’enteniment dels conceptes nuclears sovint és clau pel procés d’aprenentatge d’una matèria i per l’obertura cap a la resta de conceptes. Un dels conceptes més difícils de comprendre entre els estudiants de Farmàcia és el de la quantització de l’energia i les seves implicacions en l’espectroscòpia i, conseqüentment, en el camp de la determinació estructural.
En aquest projecte (2014PID-UB/020) explorem com l’aplicació web interactiva ESPECTRE (http://glimmer.rstudio.com/campa/espectre3a), per tot tipus de dispositius, permet explicar i apropar-se a aquest concepte científic complex amb relativa facilitat. La reciprocitat instantània facilita les connexions entre conceptes, fórmules, dades i gràfics. I no només això, també transporta la pissarra davant de cada estudiant i per tant els fa partícips actius del seu propi aprenentatge, tot d’una forma atractiva i propera als canals actuals de comunicació i aprenentatge de l’alumnat.
L’aplicació permet simular espectres d’espectroscòpia electrònica molecular i atòmica, vibracional i rotacional, així com treballar els conceptes d’absorció, emissió, espaiat energètic, unitats d’energia, efecte de la temperatura, regles de selecció, intensitat de les transicions i aspecte real dels espectres. El recurs està orientat bàsicament per a funcionar com a eina d’autoavaluació per la resolució d’exercicis i tasques d’espectroscòpia. És important ressaltar que el present objectiu va en línia amb les conclusions expressades en el famós informe Horizon (2008) on indica que les organitzacions educatives d’ensenyament superior “s’enfronten a una creixent expectativa d’oferta de serveis, continguts i documents audiovisuals per a dispositius mòbils i personals”
Implementació d’aplicacions web interactives per a l’aprenentatge de conceptes complexos (Congrés)
Podeu consultar la Vuitena trobada de professorat de Ciències de la Salut completa a: http://hdl.handle.net/2445/66524L’enteniment dels conceptes nuclears sovint és clau pel procés d’aprenentatge d’una
matèria i l’obertura cap a la resta de conceptes. Un dels conceptes més difícils de
comprendre entre els estudiants de Farmàcia és el de la quantització de l’energia i les
seves implicacions en l’espectroscòpia i conseqüentment en el camp de la determinació
estructural. En aquest projecte (2014PID-UB/020) explorem com l’aplicació web
interactiva ESPECTRE (http://glimmer.rstudio.com/campa/espectre3a), per tot tipus de
dispositius, permet explicar i apropar-se a aquest concepte científic complex amb
relativa facilitat. La reciprocitat instantània facilita les connexions entre conceptes,
fórmules, dades i gràfics. No només això, transporta la pissarra davant de cada
estudiant i per tant els fa partícips actius del seu propi aprenentatge, tot d’una forma
atractiva i propera als canals actuals de comunicació i aprenentatge de l’alumnat.
L’aplicació permet simular espectres d’espectroscòpia electrònica molecular i atòmica,
vibracional i rotacional així com treballar els conceptes d’absorció, emissió, espaiat
energètic, unitats d’energia, efecte de la temperatura, regles de selecció, intensitat de
les transicions i aspecte real dels espectres. El recurs està orientat bàsicament per a
funcionar com a eina d’autoavaluació per la resolució d’exercicis i tasques
d’espectroscòpia. És important ressaltar que el present objectiu va en línia amb les
conclusions expressades en el famós informe Horizon (2008) on indica que les
organitzacions educatives d’ensenyament superior “s’enfronten a una creixent
expectativa d’oferta de serveis, continguts i documents audiovisuals per a dispositius
mòbils i personals”
Optimization, biopharmaceutical profile and therapeutic efficacy of pioglitazone-loaded PLGA-PEG nanospheres as a novel strategy for ocular inflammatory disorders.
PURPOSE: The main goal of this study was to encapsulate Pioglitazone (PGZ), in biodegradable polymeric nanoparticles as a new strategy for the treatment of ocular inflammatory processes. METHODS: To improve their biopharmaceutical profile for the treatment of ocular inflammatory disorders, nanospheres (NSs) of PGZ were formulated by factorial design with poly (lactic-co-glycolic acid) polyethylene glycol (PLGA-PEG). Interactions drug-polymer have been carried out by spectroscopic (X-ray spectroscopy, FTIR) and thermal methods (DSC). The PGZ-NSs were tested for their in vitro release profile, cytotoxicity, and ocular tolerance (HET-CAM® test); ex vivo corneal permeation, and in vivo inflammatory prevention and bioavailability. RESULTS: The optimized system showed a negative surface charge of -13.9 mV, an average particle size (Zav) of around 160 nm, a polydispersity index (PI) below 0.1, and a high encapsulation efficiency (EE) of around 92%. According to the DSC results, the drug was incorporated into the NSs polymeric matrix. The drug release was sustained for up to 14 h. PGZ-NSs up to 10 μg/ml exhibited no retinoblastoma cell toxicity. The ex vivo corneal and scleral permeation profiles of PGZ-NSs showed that retention and permeation through the sclera were higher than through the cornea. Ocular tolerance in vitro and in vivo demonstrated the non-irritant character of the formulation. CONCLUSION: The in vivo anti-inflammatory efficacy of developed PGZ-NSs indicates this colloidal system could constitute a new approach to prevent ocular inflammation. KEYWORDS: PLGA-PEG; drug delivery; nanospheres; ocular anti-inflammatory efficacy; pioglitazon
Adaptación Metodológica al EEES de la asignatura de Técnicas Instrumentales del Grado de Farmacia de la Universidad de Barcelona
[cast] En el plan de estudios del Grado de Farmacia de la Universidad de Barcelona, la asignatura de Técnicas Instrumentales se imparte en el cuarto semestre, después de haber cursado Física, Fisicoquímica y Química Analítica. El equipo docente de la asignatura está integrado por once profesores que mediante trabajo colaborativo y adecuada coordinación organizan la docencia de la misma que se distribuye en clases teóricas y prácticas. Con el objetivo de adaptar la asignatura a las necesidades del Espacio Europeo de Educación Superior, se distribuyó en tres Bloques: I, Técnicas Espectroscópicas; II, Técnicas Electroquímicas y III, Técnicas de Separación. Las actividades teórico-prácticas se han planificado de manera secuencial. Así se inicia el ciclo con las clases teóricas del Bloque I y a continuación de manera paralela se imparten las clases prácticas del Bloque I y las clases teóricas del Bloque II y así sucesivamente, de manera que se termina la docencia con las prácticas del último Bloque. En este proceso adquiere especial relevancia tanto la formación práctica en el laboratorio como el trabajo tutorizado que debe realizar el estudiante. Se realiza un proceso de evaluación continuada teórico/práctico en cada uno de los Bloques. Se da especial relevancia a la adquisición de habilidades y destrezas que permitan una correcta realización de las prácticas de laboratorio, es decir la integración de los contenidos específicos a la aplicación de las diferentes técnicas instrumentales, la resolución de los cálculos numéricos y la interpretación de los resultados. [eng] In the new syllabus of the Pharmacy degree at the University of Barcelona, the subject Analytical Techniques is taught at the fourth semester, after the subjects Physics, Physical chemistry and Analytical chemistry. The teaching team of this subject is integrated by eleven teachers that by means of collaborative work and an appropriate coordination, organize the docent activity into practical and theoretical classes. With the aim to adapt this subject to the requirements of the European space for higher education, it has been designed in three blocs: I. Spectroscopic techniques, II. Electrochemical techniques and, III. Separation techniques, by planning the theoretical and practical activities in a sequential manner. Therefore, the cycle begins with the theory of the first bloc followed with the practice corresponding to it together with the theory of the second bloc, and so on. The course ends with the practical part of the third bloc. In this process is of great importance the tutorial work that the student should do. The evaluation of the theory and of the practical part of each bloc is done in a continuous way paying special focus on the acquisition of abilities and handiness that will allow the correct performance in the laboratory. In summary, the integration of the specific contents to the application of the different instrumental techniques, the resolution of the numerical calculations and the interpretation of the results
Memantine loaded PEGylated biodegradable nanoparticles for the treatment of glaucoma
Glaucoma is a multifactorial neurodegenerative disease associated with retinal ganglion cells (RGC) loss. Increasing reports of similarities in glaucoma and other neurodegenerative conditions have led to speculation that therapies for brain neurodegenerative disorders may also have potential as glaucoma therapies. Memantine is an N-methyl-d-aspartate (NMDA) antagonist approved for Alzheimer's disease treatment. Glutamate-induced excitotoxicity is implicated in glaucoma and NMDA receptor antagonism is advocated as a potential strategy for RGC preservation. This study describes the development of a topical formulation of memantine-loaded PLGA-PEG nanoparticles (MEM-NP) and investigates the efficacy of this formulation using a well-established glaucoma model. MEM-NPs <200 nm in diameter and incorporating 4 mg mL−1 of memantine were prepared with 0.35 mg mL−1 localized to the aqueous interior. In vitro assessment indicated sustained release from MEM-NPs and ex vivo ocular permeation studies demonstrated enhanced delivery. MEM-NPs were additionally found to be well tolerated in vitro (human retinoblastoma cells) and in vivo (Draize test). Finally, when applied topically in a rodent model of ocular hypertension for three weeks, MEM-NP eye drops were found to significantly (p < 0.0001) reduce RGC loss. These results suggest that topical MEM-NP is safe, well tolerated, and, most promisingly, neuroprotective in an experimental glaucoma model
Hábitat reproductor de Salamandra salamandra (Linnaeus, 1758) en el Noroeste de la Región de Murcia (S.E. Península Ibérica): Distribución actualizada
Development of fluorometholone-loaded PLGA nanoparticles for treatment of inflammatory disorders of anterior and posterior segments of the eye
The main objective of this study was the development and optimization of fluorometholone-loaded PLGA nanoparticles for the treatment of inflammatory conditions of the eye. Design of experiments was used to obtain nanoparticles with the best physicochemical characteristics. The optimized nanoparticles containing 1.5 mg·mL-1 of fluorometholone showed a negative surface charge (-30 mV) and an average size below 200 nm being suitable for ocular administration. Drug-polymer interaction studies confirmed no new bonds were formed during the synthesis. Nanoparticles performance was assessed with biopharmaceutical behavior studies, ocular tolerance, anti-inflammatory efficacy and bioavailability. The biopharmaceutical behavior of the drug from nanoparticles was adjusted to hyperbola order showing a significantly greater permeation in the cornea than in the sclera. The optimized formulation had significantly greater anti-inflammatory effects than the commercial formulation. In addition, nanoparticles increased drug penetration toward the vitreous. Polymeric nanoparticles of fluorometholone could provide a suitable alternative for the treatment of inflammatory disorders of the anterior and posterior segments of the eye against of conventional topical formulations. KEYWORDS: Drug delivery; Fluorometholone; Nanoparticles; Ocular anti-inflammatory; PLGA; Permeatio
Development of fluorometholone-loaded PLGA nanoparticles for treatment of inflammatory disorders of anterior and posterior segments of the eye.
The main objective of this study was the development and optimization of fluorometholone-loaded PLGA nanoparticles for the treatment of inflammatory conditions of the eye. Design of experiments was used to obtain nanoparticles with the best physicochemical characteristics. The optimized nanoparticles containing 1.5 mg·mL-1 of fluorometholone showed a negative surface charge (-30 mV) and an average size below 200 nm being suitable for ocular administration. Drug-polymer interaction studies confirmed no new bonds were formed during the synthesis. Nanoparticles performance was assessed with biopharmaceutical behavior studies, ocular tolerance, anti-inflammatory efficacy and bioavailability. The biopharmaceutical behavior of the drug from nanoparticles was adjusted to hyperbola order showing a significantly greater permeation in the cornea than in the sclera. The optimized formulation had significantly greater anti-inflammatory effects than the commercial formulation. In addition, nanoparticles increased drug penetration toward the vitreous. Polymeric nanoparticles of fluorometholone could provide a suitable alternative for the treatment of inflammatory disorders of the anterior and posterior segments of the eye against of conventional topical formulations. KEYWORDS: Drug delivery; Fluorometholone; Nanoparticles; Ocular anti-inflammatory; PLGA; Permeatio
Development of fluorometholone-loaded PLGA nanoparticles for treatment of inflammatory disorders of anterior and posterior segments of the eye.
The main objective of this study was the development and optimization of fluorometholone-loaded PLGA nanoparticles for the treatment of inflammatory conditions of the eye. Design of experiments was used to obtain nanoparticles with the best physicochemical characteristics. The optimized nanoparticles containing 1.5 mg·mL-1 of fluorometholone showed a negative surface charge (-30 mV) and an average size below 200 nm being suitable for ocular administration. Drug-polymer interaction studies confirmed no new bonds were formed during the synthesis. Nanoparticles performance was assessed with biopharmaceutical behavior studies, ocular tolerance, anti-inflammatory efficacy and bioavailability. The biopharmaceutical behavior of the drug from nanoparticles was adjusted to hyperbola order showing a significantly greater permeation in the cornea than in the sclera. The optimized formulation had significantly greater anti-inflammatory effects than the commercial formulation. In addition, nanoparticles increased drug penetration toward the vitreous. Polymeric nanoparticles of fluorometholone could provide a suitable alternative for the treatment of inflammatory disorders of the anterior and posterior segments of the eye against of conventional topical formulations. KEYWORDS: Drug delivery; Fluorometholone; Nanoparticles; Ocular anti-inflammatory; PLGA; Permeatio