Genes involved in the regulation of vascular homeostasis determine renal survival rate in patients with chronic glomerulonephritis

In this study, we analyzed association of genes of vascular homeostasis with hypertension and renal survival of CGN patients. The study sample included 238 patients with CGN and 304 healthy subjects of population control. Ten polymorphisms of ten genes of vascular homeostasis were genotyped through polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP) analysis and TaqMan assays. Association of the genotypes with renal survival was analyzed by the Kaplan-Meier estimato

Evaluation of the combining ability of CMS lines in crosses with samples of grain sorghum and Sudan grass

Received: April 2nd, 2021 ; Accepted: July 28th, 2021 ; Published: August 3rd, 2021 ; Correspondence: [email protected] versatility of use, species diversity, and high drought resistance have ensured the demand for sorghum among agricultural crops in the world. Currently, the most promising direction of breeding is the creation of F1 sorghum hybrids, which is based on the identification and selection of parental forms with high combining ability. In this paper, the combining ability analysis crosses of two hybridization schemes: grain×grain sorghum and grain sorghum×Sudan grass was carried out using topcross method. Gene action governing inheritance of a particular trait can be measured in terms of general and specific combining ability estimates, where general combining ability effects are mostly indicative of additive gene action (ms (GCA)/ms (SCA)>1)and specific combining ability effects - non-additive (ms (GCA)/ms (SCA)<1). Genes with an additive effect participate in the genetic control of breeding-valuable traits, which is proven byms(GCA)/ms (SCA) ratios changing within1.10-28.01 range. It is advisable to involve CMS-lines A3 Feterita 14, A2 KVV 114 and A2 O-1237 to create high-yielding hybrids of grain sorghum; highly productive sorghum-sudangrasshybridss - lines L-106, L-143 and Anastasiya, Kinelskaya 100, Elegiya variety samples; with a high share of grain in the total biomass – Allegoriya, Krasnodarskaya 75 and Zonalskaya 6 variety samples. CMS lines are distinguished by high and average CA values both in crosses with samples of grain sorghum and Sudan grass: by weight of 1,000 grains - A2 O-1237; by seed yield and weight of 1,000 grains - A2 KVV 114

The role of chemokine genes in the formation of terminal stage of chronic renal failure

The data on the role of chemokine genes (+1931A/T CCL4, A/G CXCL11 (rs4512021), -403A/G CCL5, C/G CCL2 (rs2857657), -801G/A CXCL12) in the formation of terminal stage of chronic renal failure, in patients with chronic glomerulonephritis, is presented in the work. It was established, that the allele A CXCL11 (rs4512021) (OR = 1.65) was the marker for the development of terminal stage of chronic renal insufficiency, and the genotype GG CXCL11was a protective factor for the development of terminal stage of chronic renal failure (OR = 0.22

The effectiveness of glucocorticoid therapy in patients with chronic glomerulonephritis, depending on the polymorphic markers of cytokine genes

The paper presents the results of a study of interrelationships of polymorphic cytokine loci (rs1800629 TNFα, rs909253 Ltα, rs767455 TNFR1 and rs1800469 TGFβ-1) with the features of glucocorticoid therapy in patients with chronic glomerulonephriti

Study of Biological Properties of Effective Pesticides Destructor Pseudomonas Putida P2

A promising approach for remediation of contaminated soils is the use of biological products based on microorganisms-destructors. Of particular interest are the strains that can decompose several types of pollutants. Potential strains-destructors should be not only effective but also adapted for certain soil and climatic conditions. In addition, when introducing a significant amount of biological agents into the ecosystem, the lack of toxicological and pathogenic effects on plants, animals, and humans is particularly important. The object of the study is the destructor strain of sim-triazine herbicide of prometrin Pseudomonas putida P2. The study explores the ability of strain to use various pesticides as the only carbon source, the effect of bacterial culture on seed germination, biotesting of strain on toxicity using earthworms. Besides, the study presents the phytopathogenic factors and enzymes causing bacteria invasiveness into animals and humans. The determination of dehydrogenase activity of prometrin destructor strain shows that this strain can also use active ingredients of pesticide malathion, HCH, and 4,4-DDT as only sole carbon source. It is also established that the studied strain Pseudomonas putida P2 increases the germination of seeds of the variety Saratovskaya 70 and carrots of the variety Samson and does not possess pathogenicity factors. The strain biotesting on the earthworms revealed no acute toxicity of the culture. The analysis of data obtained from the model pollution shows that the introduction of the destructor of Prometrium Pseudomonas putida P2 into the soil can create favorable conditions for vital activity and reproduction of earthworms

Associations of cytokines genetic polymorphisms with hypertension progress

The article presents results of comparative analysis of cytokines genes polymorphous variants occurrence among hypertension patients with burdened familial history regarding this disease and in a control grou

A rare combination of noncompact myocardium and coronary dilatation with type 1 neurofibromatosis

The article describes the observation of a child with a very rare combination of noncompact left ventricular myocardium and coronary dilatation in type 1 neurofibromatosis (NF1). The first of these complications, undifferentiated cardiomyopathy, also known as noncompact myocardium, is not described in combination with nF 1 in available literature, although cases of hypertrophic cardiomyopathy in patients with NF 1 have been previously reported. Dilatation of the coronary arteries also refers to infrequent manifestations of vascular pathology against the background of neurofibromatosis, and the combination of all three signs (NF 1, noncompact myocardium and coronary dilatation) has not yet been reported by other authors. When examining the patient, we found the characteristic signs of NF 1 (foci of "coffee" pigmentation on the skin, multiple nevuses, fibroids of the forearm, cognitive disorders), electrocardiogram features of left ventricular hypertrophy, negative T-tooth and ST-segment displacement 1 mm below the isoline in Leads V4-6, in echocardiography - pronounced trabecularity, thinning of the compact myocardium layer and a "spongy myocardium" in the region of the left ventricle apex, dilatation of the coronary arteries. With magnetic resonance imaging, signs of noncompact myocardium of the left ventricle were found, with selective coronary angiography - slowing of the coronary blood flow. The patient receives the permanent treatment for heart failure and aspirin, his condition remains stable for 4 years of follow-u

Ultrasound diagnostics of left ventricular noncompaction

The clinical cases of rare, genetically heterogeneous cardiomyopathy are described - the left ventricular noncompaction (LVNC) cardiomyopathy for four children (three boys and one girl) in age from two months to 6 years. Description of echocardiography signs is presented in accordance with modern diagnostic criteria. Noncompaction of the left ventricle is recognized in three patients, of the left and right ventricle - in one. In three patients out of four, LVNC was a separate disease, and yet in one it was associated with neurofibromatosis type 1, in combination with dilatation of coronary arteries and pericardial effusion. Three of four patients were admitted with congestive heart failure (CHF) of New York Heart Association (NYHA) functional class 3-4 (FC), and one - with CHF 2 FC. The echocardiograms are brought with description of characteristic features of LVNC (hypertrabecularity, spongy myocardium on the apex of the left ventricle, typical correlation of incompact and compact layer from 2.0 to 2.6). All four patients received treatment for CHF (diuretics, beta-blockers, captopril, in one case - digoxin); two out of four got antithrombotic therapy (aspirin, warfarin). Results of follow-up observation of the patients are described with the estimation of echocardiography signs, including dilatation of coronary arteries. Left ventricular ejection fraction - both by Simpson and Teicholz - increased in two patients, with therapy of chronic heart failure at a follow-up visits over > 5 years simultaneously with the decline of severity of CHF. Another two patients have been examined from 2 months to 5 years and have had CHF 3 FC; their left ventricular ejection fraction increased too, but cardiomegaly and right ventricular heart failure preserved

Estimate or the effectiveness of intelligent information system of early diagnosis and prognosis of cardiovascular disease

This article describes a new approach to the implementation of the training program of risk probability of coronary heart disease (CHD) remote evaluation. It describes an example of training sample formation based on the statistical analysis of medical data. The proposed solution will allow medical personnel to carry out the personification of cardiac services and first of all, preventive orientation. Also a method for assessing the effectiveness of the program of expert system "ARM-Cardiologist" on the results of ongoing clinical trials is providedyesБелгородский государственный университе

Clinical and genetic research of chronic glomerulonephritis

The paper presents data on interaction of candidate genes (S311C PON2, (-6)A/G AGT, (-1166)A/CAGTR1, (-592)C/A IL-10, VNTR IL-1Ra, T113M IL-9, K198N EDN1, (+46)G/A ADRB2, G/A GNB3 (rs.2301339)) with oligogenic and continuous characters of chronic glomerulonephriti