Glutathione S Transferases M1 and T1 Genetic Variants are Associated with Coronary Artery Ectasia

29th Turkish Cardiology Congress of the Turkish-Society-of-Cardiology (TSC) with International Participation -- OCT 26-29, 2013 -- Antalya, TURKEYWOS: 000329858400026…Turkish Soc Cardio

Эксплуатация системы транспорта продукта на нефтегазоконденсатном месторождении Томской области

Объектом исследования является система транспорта продукта на нефтегазоконденсатном месторождении Томской области. Цель работы: анализ особенностей системы транспорта нефти на нефтегазоконденсатном месторождении Томской области. В процессе работы проводился: анализ характеристик внутрипромысловых и технологических трубопроводов, анализ системы хранения продукта и его транспортировки, а также обеспечивающего технологический процесс оборудования. Приведены мероприятия по техническому обслуживанию нефтепроводов и их защите от коррозии, определена расчетная и отбраковочная толщина стенок труб, проведен расчет остаточного ресурса трубопровода по минимальной вероятной толщине стенок труб.The object of study is the product transport system in the oil and gas condensate field of the Tomsk region. Objective: to analyze the features of the oil transportation system in the oil and gas condensate field of the Tomsk region. In the process of work was carried out: analysis of the characteristics of infield and technological pipelines, analysis of the product storage system and its transportation, as well as equipment ensuring the technological process. Measures for the maintenance of oil pipelines and their protection against corrosion are given, the calculated and rejected wall thicknesses of the pipes are determined, and the residual life of the pipeline is calculated from the minimum probable pipe wall thickness

The Effects Of Vaccinium Myrtillus On Antioxidant System and Lipid Peroxidation in Experimental Diabetic Rats Model

Aim:Vaccinium myrtillus (VM) is popularly known as blueberries used as a therapeutic in many diseases, especially diabetes. VM has an extensive habitat in Turkey and America. In this study, we aimed to investigate the effect of Vaccinium myrtillus on lipid peroxidation, antioxidant system and plasma glucose levels in streptozotocin (STZ) induced diabetic rats.Materials and Methods:32 adult male Sprague-Dawley rats were divided into 4 groups, including 8 rats in each group. Groups were determined as control (C), streptozotocin (STZ), Vaccinium myrtillus (VM) and Vaccinium myrtillus + streptozotocin (STZ+VM). After preparation of STZ contains citrate buffer (0.1 M, pH 4.5), containing 55 mg/kg, it was applied to the rats as intraperitoneal and single dose. Extracts were given as oral gavage according to the group of animals every day during 10 days (100mg / kg). On day 11, diabetes were introduced by streptozocin (55mg / kg) according to animal groups. Blood glucose was measured from the queue. The catalase that is an antioxidant enzyme, activity was measured, by the Aebi method. Lipid peroxidation product that malondialdehyde (MDA) content was studied by spectrophotometric method according to Draper and Hadley method. The determination of supernatant of the resulting protein extracts of tissue homogenates was made by determining by the Lowry method. The results are evaluated by ANOVA, p<0.05 were considered significant.Results:There was a significant increase in liver CAT values in VM group (p <0.05). When the liver MDA levels were examined, there were significant differences between STZ and VM, STZ and C. When the kidney MDA levels were compared, MDA levels in STZ group were significantly higher than VM (p <0.05).Conclusion:The oxidative stress increase in rats by creation of diabetes. The applying of Vaccinium myrtillus to the diabetic rats reduces oxidative stress and this improves the general state of the rats. More studies are needed to determine more clearly the antidiabetic and antioxidant effects of Vaccinium myrtillus

The Effects of Vaccinium Myrtillus on Antioxidant System and Lipid Peroxidation in Experimental Diabetic Rats Model

Amaç: Yaban mersini olarak bilinen ve halk arasında diyabet başta olmak üzere birçok hastalıkta tedavi edici olarak kullanılan Vaccinium myrtillus, Türkiye’de yaygın bir yetişme alanına sahiptir. Bu araştırmada, Vaccinium myrtillus’un, streptozotosin (STZ) ile diyabet oluşturulmuş sıçanlarda, lipit peroksidasyonu, antioksidan sistem ve plazma glikoz düzeyi üzerine etkilerinin incelenmesi amaçlandı. Materyal ve Metot: 32 adet Sprague-Dawley yetişkin erkek sıçan, her grupta 8’er adet olmak üzere 4 gruba ayrıldı. Gruplar: Kontrol (K), streptozotosin (STZ), Vaccinium myrtillus (VM), ve Vaccinium myrtillus + streptozotosin (VM + STZ) verilecek şekilde belirlendi. STZ sitratlı tampon (0,1 M, pH 4,5) içerisinde 55 mg/kg olacak şekilde hazırlandıktan sonra sıçanlara tek doz ve intraperitoneal uygulandı. Hazırlanan ekstre hayvanlara gruplarına göre 10 gün boyunca her gün gavaj olarak verildi (100 mg/kg). 11. gün hayvan gruplarına göre streptozosin ile diyabet oluşturuldu (55 mg/kg). Kuyruktan alınan kan ile glikoz ölçüldü. Antioksidan bir enzim olan katalaz, Aebi’nin metodu kullanılarak;lipit peroksidasyon ürünü olan MDA, Draper ve Hadley’in spektrofotometrik metoduyla;protein tayini ise doku homojenatlarından oluşan ekstratların süpernatantlarının Lowry’nin yöntemine göre belirlenmesiyle hesaplandı. Sonuçlar ANOVA testi ile değerlendirilip, p<0,05 değerler anlamlı kabul edilmiştir. Sonuçlar: STZ ile diyabet yapılmadan önce VM verilen grupta karaciğer CAT değerlerinde anlamlı bir artış saptandı. Karaciğer MDA değeri incelendiğinde STZ ile VM arasında ve STZ ile K arasında anlamlı farklılık gözlendi. Böbrek MDA değerinde, STZ ile VM karşılaştırıldığında STZ grubunda MDA değeri yüksek bulundu (p <0,05). Tartışma: Sıçanlarda diyabet oluşturulmasıyla oksidatif stres artmaktadır. Diyabetik sıçanlara Vaccinium myrtillus verilmesi oksidatif stresi azaltmakta ve bu durum sıçanın genel durumunu iyileştirmektedir. Vaccinium myrtillus’un antidiyabetik ve antioksidan etkilerinin daha net belirlenmesi için daha çok çalışmaya ihtiyaç duyulmaktadır.Aim: Vaccinium myrtillus (VM) is popularly known as blueberries used as a therapeutic in many diseases, especially diabetes. VM has an extensive habitat in Turkey and America. In this study, we aimed to investigate the effect of Vaccinium myrtillus on lipid peroxidation, antioxidant system and plasma glucose levels in streptozotocin (STZ) induced diabetic rats. Materials and Methods: 32 adult male Sprague-Dawley rats were divided into 4 groups, including 8 rats in each group. Groups were determined as control (C), streptozotocin (STZ), Vaccinium myrtillus (VM) and Vaccinium myrtillus + streptozotocin (STZ+VM). After preparation of STZ contains citrate buffer (0.1 M, pH 4.5), containing 55 mg/kg, it was applied to the rats as intraperitoneal and single dose. Extracts were given as oral gavage according to the group of animals every day during 10 days (100mg / kg). On day 11, diabetes were introduced by streptozocin (55mg / kg) according to animal groups. Blood glucose was measured from the queue. The catalase that is an antioxidant enzyme, activity was measured, by the Aebi method. Lipid peroxidation product that malondialdehyde (MDA) content was studied by spectrophotometric method according to Draper and Hadley method. The determination of supernatant of the resulting protein extracts of tissue homogenates was made by determining by the Lowry method. The results are evaluated by ANOVA, p<0.05 were considered significant. Results: There was a significant increase in liver CAT values in VM group (p <0.05). When the liver MDA levels were examined, there were significant differences between STZ and VM, STZ and C. When the kidney MDA levels were compared, MDA levels in STZ group were significantly higher than VM (p <0.05). Conclusion: The oxidative stress increase in rats by creation of diabetes. The applying of Vaccinium myrtillus to the diabetic rats reduces oxidative stress and this improves the general state of the rats. More studies are needed to determine more clearly the antidiabetic and antioxidant effects of Vaccinium myrtillus

The effects of midazolam and etomidate on the antioxidant system in the rat liver

This study was conducted to investigate the histopathological and biochemical effects of midazolam and etomidate, which are intravenous anesthetics used in surgical operations, on rat liver. There were 3 study groups each consisting of 9 animals: the control, midazolam, and etomidate groups. Midazolam and etomidate were administered 10 times with 1-day intervals. At the end of the study, tissues were taken for histological examination and biochemical analysis. We determined superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) levels in the liver. There was a slight increase in MDA level in the midazolam-exposed group when compared with the controls, and this was not statistically significant. However, SOD and CAT activities were significantly increased in the midazolam group. We found slight damage in histological sections in parallel to biological findings. MDA levels were significantly increased in the etomidate group and SOD and CAT activities were also prominent in this group. More histological changes like hepatocyte degeneration, vascular congestion, and mononuclear cell infiltration were observed in the etomidate group and this was statistically significant. We can say that these intravenous anesthetic agents may cause dose-dependent damage in tissue. Therefore, we think that one must be cautious of the administration duration and dose levels of anesthetics during surgical operations

Nitric oxide levels and superoxide dismutase enzyme activity in synovial fluid of patients with knee osteoarthritis

WOS: 000253939600003Objectives: We investigated nitric oxide (NO) levels and superoxide dismutase (SOD) enzyme activity in synovial fluid of patients with primary knee osteoarthritis (OA). Patients and methods: The study included 36 patients (31 females, 5 males; mean age 63 years; range 48 to 88 years) with knee OA according to the diagnosis criteria of American College of Rheumatology. Radiographic severity of OA was assessed according to the Kellgren-Lawrence grading system. Synovial fluid samples were taken by needle aspiration before knee arthroplasty or hyaluronic acid injection and stored at -80 degrees C in Eppendorf tubes. Nitrite and nitrate levels were determined by the spectrophotometric method based on the Griess reaction. Total SOD activity was determined by the spectrophotometric method. The results were compared with those of 10 controls (6 females, 4 males; mean age 49 years; range 26 to 70 years) who clinically and radiographically had normal knees, but underwent arthroscopic examination for knee pain of unknown origin. Results: Compared to controls, NO levels were significantly higher and SOD activity was significantly lower in patients with OA (p 0.05). Conclusion: Our data suggest that NO acts as a potent mediator of cartilage damage and SOD as an antioxidant mediator in OA. Further studies are needed to clarify the clinical significance of these parameters

Nitric oxide levels and superoxide dismutase enzyme activity in synovial fluid of patients with knee osteoarthritis

Amaç: Diz osteoartritli (OA) hastaların eklem sıvısında nitrik oksit (NO) düzeyi ve süperoksit dismutaz (SOD) enzim aktivitesi araştırıldı. Hastalar ve yöntemler: Amerikan Romatoloji Birliği OA ölçütlerine uyan 36 hasta (31 kadın, 5 erkek; ort. yaş 63; dağılım 48-88) çalışmaya alındı. Hastaların diz grafileri Kellgren-Lawrence radyografik sınıflamasına göre derecelendirildi. Sinovyal sıvı örnekleri, OA nedeniyle diz protezi veya hiyaluronik asit enjeksiyonu yapılan hastalardan girişimden önce iğne aspirasyonuyla toplandı, daha sonra Eppendorf tüplerine aktarılarak -80 °C’de saklandı. Sinovyal sıvıdaki nitrit ve nitrat değerleri Griess reaksiyonuna dayalı spektrofotometrik yöntemle belirlendi. Toplam SOD aktivitesi de spektrofotometrik yöntemle ölçüldü. Sonuçlar, diz eklemleri klinik ve radyografik olarak normal bulunan, ancak nedeni bilinmeyen diz ağrısından dolayı artroskopi uygulanan 10 hastayla (6 kadın, 4 erkek; ort. yaş 49; dağılım 26-70) karşılaştırıldı. Bulgular: Osteoartritli hasta grubunda NO düzeyi kontrol grubuna göre anlamlı derecede yüksek, SOD aktivitesi düşük bulundu (p0.05). Sonuç: Bulgularımız NO’nun kıkırdak yıkım mediyatörü, SOD’nin ise antioksidan mediyatör olduğunu düşündürmektedir. Bu değerlerin klinik önemini aydınlatmak için daha kapsamlı çalışmalara ihtiyaç vardır.Objectives: We investigated nitric oxide (NO) levels and superoxide dismutase (SOD) enzyme activity in synovial fluid of patients with primary knee osteoarthritis (OA). Patients and methods: The study included 36 patients (31 females, 5 males; mean age 63 years; range 48 to 88 years) with knee OA according to the diagnosis criteria of American College of Rheumatology. Radiographic severity of OA was assessed according to the Kellgren-Lawrence grading system. Synovial fluid samples were taken by needle aspiration before knee arthroplasty or hyaluronic acid injection and stored at -80 &deg;C in Eppendorf tubes. Nitrite and nitrate levels were determined by the spectrophotometric method based on the Griess reaction. Total SOD activity was determined by the spectrophotometric method. The results were compared with those of 10 controls (6 females, 4 males; mean age 49 years; range 26 to 70 years) who clinically and radiographically had normal knees, but underwent arthroscopic examination for knee pain of unknown origin. Results: Compared to controls, NO levels were significantly higher and SOD activity was significantly lower in patients with OA (p&lt;0.001). No significant differences were found between radiographically graded groups with regard to NO level and SOD activity (p&gt;0.05). Conclusion: Our data suggest that NO acts as a potent mediator of cartilage damage and SOD as an antioxidant mediator in OA. Further studies are needed to clarify the clinical significance of these parameters

Probiyotik Mayanın Yüksek Kolesterol İçeren Diyetle Beslenen Sıçanlarda Kolesterolü Düşürücü Ajan Olarak Kullanılabilirliğinin Araştırılması

Yüksek kolesterol asimilasyon yeteneğine sahip probiyotik maya Cryptococcus humicola M5-2 suşu, geleneksel olarak üretilmiş bir peynirden izole edilmiş ve in vivo ortamda incelenmiştir. Çalışmada, C. humicola M5-2 suşunun in vivo ortamda kolesterolü asimile etme yeteneği incelenmiş ve kolesterolce zengin diyetle beslenen sıçanlarda bu suş ile beslemenin serum toplam kolesterol, HDL/LDL kolesterol ve trigliserit seviyelerine etkisi belirlenmiştir. C. humicola nın besiyeri içersindeki kolesterolü asimile etme oranı %73.33tür. Hayvanların beslenmesinde suş iki farklı dozda kullanılmıştır (yüksek ve düşük doz, sırasıyla %2 ve %0.1 liyofilize suş içermektedir). Deneme grupları karşılaştırıldığında, düşük doz besleme grubu daha olumlu sonuçlar göstermiştir. Serum analiz sonucuna göre trigliserit ve toplam kolesterol düzeyi sırasıyla %25 ve %1.34 oranında düşme göstermiştir. Özellikle trigliserit düzeyindeki düşme oranı çoğu in vivo çalışmada rastlanmamıştır. Elde edilen suşun fermente gıdalar ile beraber tüketiminin sağlık üzerine iyileştirici etkiler yaratacağı düşünülmektedir.Probiotic yeast, Cryptococcus humicola M5-2 strain, which has high cholesterol assimilation feature and isolated from traditionally produced cheese, was investigated in vivo. This study was conducted to determine cholesterol assimilation ability of C. humicola M5-2 in vivo and specify the effect of the strain on serum total cholesterol, HDL/LDL cholesterol and triglycerides levels in rats fed on cholesterol-enriched diet. C. humicola had the ability to assimilate cholesterol at the rate of 73.33% in media. The strain was used with two different concentrations in animal feed (high and low doses containing 2% and 0.1% lyophilized strains, respectively). When the treatment groups were compared, low dose feeding group had the positive results in terms of testing values. According to the results of serum analysis, triglyceride and total cholesterol level were decreased by 25% and 1.34% respectively. Especially, decreasing the percentage rate of triglyceride has not obtained in other in vivo studies. It is thought that health promoting effect will be possible when the obtained isolate is consumed with fermented foods.WOS:0003626669000102-s2.0-8493475327

Nitric oxide levels and superoxide dismutase enzyme activity in synovial fluid of patients with knee osteoarthritis

Amaç: Diz osteoartritli (OA) hastaların eklem sıvısında nitrik oksit (NO) düzeyi ve süperoksit dismutaz (SOD) enzim aktivitesi araştırıldı. Hastalar ve yöntemler: Amerikan Romatoloji Birliği OA ölçütlerine uyan 36 hasta (31 kadın, 5 erkek; ort. yaş 63; dağılım 48-88) çalışmaya alındı. Hastaların diz grafileri Kellgren-Lawrence radyografik sınıflamasına göre derecelendirildi. Sinovyal sıvı örnekleri, OA nedeniyle diz protezi veya hiyaluronik asit enjeksiyonu yapılan hastalardan girişimden önce iğne aspirasyonuyla toplandı, daha sonra Eppendorf tüplerine aktarılarak -80 °C’de saklandı. Sinovyal sıvıdaki nitrit ve nitrat değerleri Griess reaksiyonuna dayalı spektrofotometrik yöntemle belirlendi. Toplam SOD aktivitesi de spektrofotometrik yöntemle ölçüldü. Sonuçlar, diz eklemleri klinik ve radyografik olarak normal bulunan, ancak nedeni bilinmeyen diz ağrısından dolayı artroskopi uygulanan 10 hastayla (6 kadın, 4 erkek; ort. yaş 49; dağılım 26-70) karşılaştırıldı. Bulgular: Osteoartritli hasta grubunda NO düzeyi kontrol grubuna göre anlamlı derecede yüksek, SOD aktivitesi düşük bulundu (p0.05). Sonuç: Bulgularımız NO’nun kıkırdak yıkım mediyatörü, SOD’nin ise antioksidan mediyatör olduğunu düşündürmektedir. Bu değerlerin klinik önemini aydınlatmak için daha kapsamlı çalışmalara ihtiyaç vardır.Objectives: We investigated nitric oxide (NO) levels and superoxide dismutase (SOD) enzyme activity in synovial fluid of patients with primary knee osteoarthritis (OA). Patients and methods: The study included 36 patients (31 females, 5 males; mean age 63 years; range 48 to 88 years) with knee OA according to the diagnosis criteria of American College of Rheumatology. Radiographic severity of OA was assessed according to the Kellgren-Lawrence grading system. Synovial fluid samples were taken by needle aspiration before knee arthroplasty or hyaluronic acid injection and stored at -80 &deg;C in Eppendorf tubes. Nitrite and nitrate levels were determined by the spectrophotometric method based on the Griess reaction. Total SOD activity was determined by the spectrophotometric method. The results were compared with those of 10 controls (6 females, 4 males; mean age 49 years; range 26 to 70 years) who clinically and radiographically had normal knees, but underwent arthroscopic examination for knee pain of unknown origin. Results: Compared to controls, NO levels were significantly higher and SOD activity was significantly lower in patients with OA (p&lt;0.001). No significant differences were found between radiographically graded groups with regard to NO level and SOD activity (p&gt;0.05). Conclusion: Our data suggest that NO acts as a potent mediator of cartilage damage and SOD as an antioxidant mediator in OA. Further studies are needed to clarify the clinical significance of these parameters