Varying patterns of protein synthesis in bread wheat during heat shock

High temperatures during seedling growth are considered as one of the factors that can modify surviving properties in wheat ( Triticum aestivum L.) plant. This work attempts to evaluate the heat shock responses of seedling of winter wheat (Bezostaya-1) using growth parameters (seedling length, embryonal root length and embryonal root number), membrane stability index (MSI) and two dimensional (2D) gel electrophoresis analysis of heat shock proteins (HSPs) during heat shock. Seedlings grown until first leaf opening at controlled conditions (23 °C, 200 μmol m −2 s −1 , 16h day/8h night, 50–60% humidity) were exposed to 37 °C or 45 °C high temperatures for 2, 4 and 8 hours. While 37 °C did not cause any significant change, 45 °C heat treatments caused significant decrease in terms of seedling and root length, and leaf MSI for all exposure times. However, all the plants from 45 °C heat treatments continued to grow during recovery period. 2D protein analysis indicated that 37 °C, 8 hours exposure caused stronger and more diverse heat shock response than the other treatments, followed by 37 °C, 4 hours, 45 °C, 8 hours, 45 °C, 4 hours, 45 °C, 2 hours treatments. 5 protein spots, ranging from 6–7.8 p I (isoelectric point) and 27–31.7 kDA molecular weight, were expressed at 37 °C, 2 hours and continued at 37 and 45 °C for all exposure times. This suggests that these early proteins and other newly synthesized proteins may have protective effects at 37 and 45 °C and provide plants for healthy growth during the recovery period

Papillon-Lefevre syndrome. Analysis of neutrophil chemotaxis

PAPILLON-LEFEVRE SYNDROME (PLS) IS DESCRIBED as the association of palmar-plantar hyperkeratosis with precocious periodontal disease which results in exfoliation of primary and permanent dentitions. This study was planned to assess the chemotaxis of peripheral blood neutrophils in 7 patients (3 females and 4 males) with Papillon-Lefevre syndrome. The neutrophil chemotaxis was analyzed using the zymosan activated serum (ZAS) assay. Chemotaxis and spontaneous migration measurements were compared to those of the healthy control subjects. The peripheral blood neutrophil chemotaxis and spontaneous migration were depressed in all patients with Papillon-Lefevre syndrome. The decreased chemotaxis of peripheral blood neutrophils strongly suggests that the neutrophils may act as one of the important key determinants in the pathogenesis of severe periodontal destruction in patients with PLS

Deposition of ZnO thin films by RF&DC magnetron sputtering on silicon and porous-silicon substrates for pyroelectric applications

In this study, the temperature response of ZnO thin film is investigated in an attempt to enhance its pyroelectric performance. The film is formed on PS and Si substrates utilizing RF&DC magnetron sputtering deposition technique. The outcome of study reveals a pyroelectric coefficient observed from ZnO film on PS which is 40 times higher than that on Si and a pyroelectric voltage as high as 2.4 V due to PS's profound effect on film formation, large surface to volume area and low thermal conductivity. Thus, this study can lead the way to a robust, reliable and more efficient pyroelectric operation of ZnO with employment of PS structure. © 2017 Elsevier B.V

In vitro release of elements from prosthodontic base metal alloys: Effect of protein-containing biologic environments

WOS: 000237769900005PubMed ID: 16752620Purpose: This study aimed to investigate the release of nickel, copper, zinc, cadmium, magnesium, and lead from prosthodontic base metal alloys into protein-containing biologic solutions. Materials and Methods: Dissolution experiments were conducted in either 3% Dulbecco's Modified Eagle Medium or 3% bovine serum albumin solutions for 7 weeks. Results: The protein-containing dissolution solutions and dissolution time did not have a significant effect on release of elements from the alloys (Kruskal-Wallis, P > .05). Conclusions: The amount of the released elements was well below the dietary intake levels of these elements. This study is important given the widespread use of the base metal alloys and the continuing public concerns/questions regarding the health benefits/risks associated with these materials

Review of 101 benign epithelial, mesenchymal and mixed odontogenic tumours

WOS: 00040873540123

Biomechanical validation of a modified genioplasty distractor

2-s2.0-85103571960Purpose: Genioplasty is a surgical procedure that is used to enhance the shape and appearance of the chin. It can be performed alone or in combination with other jaw-related surgeries either for medical or cosmetic purposes. Recently many studies have been reported for mandibular reconstruction with distraction osteogenesis. However, these distractors can cause some complications such as incorrect prolongation of hard tissues due to the lack of guiding section. The purpose of this study is to manufacture a novel genioplasty distractor and measure its biomechanical stability and reliability for different activation lengths in mandibular bone. Methods: The modified genioplasty distractor was manufactured from grade 2 and grade 5 (Ti6AI4V) titanium alpha + beta alloy which was biocompatible and adequately rigid for possible in situ application in the future and a sample holder was manufactured for compressive strength testing. Results: Test results showed that our modified genioplasty distractors withstood 300 N compression force for activation lengths from 0 to 11 mm. Recorded stress values were in the range of 0.110 MPa to 0.389 MPa. Conclusion: The modified genioplasty distractor developed and tested here is a promising surgical tool that has the potential to reduce genioplasty related complications especially in demanding cases. © 202