Preference and performance of Phyllocnistis citrella Stainton (Lepidoptera:Gracillariidae) on three citrus hosts: Laboratory and field assessment

The relationship between preference and performance is crucial to the ecology and evolution of plant-insect interactions. Oviposition preference and offspring performance were evaluated for a citrus pest, the leafminer Phyllocnistis citrella (Lepidoptera: Gracillariidae), on three of its host plants: lemon (Citrus limon L. Burm.), orange (Citrus sinensis L. Osbeck), and grapefruit (Citrus paradisi Macfadyen) in Tucumán province (northwest Argentina). Choice and no-choice tests were performed in open and enclosed environments, and performance parameters (development time, survival, pupal size, and sex ratio) were estimated from laboratory rearing and 3-yr field sampling data. Parasitism rates were studied in laboratory choice test and field assessments. Preference trends were inconsistent, with lemon receiving more eggs in some tests, whereas no preference was observed in others. Patterns of host use in the field did not show significant differences among species. Leafminer performance, including parasitism and predation rates, was generally homogeneous among host plants. From these results, lemon, orange, and grapefruit seem to represent intrinsically similar resources for P. citrella populations in northwest Argentina, a trend that was accompanied by a lack of consistent oviposition preferences in foraging females. Ecological conditions might be more important than physiological adaptation in shaping a probably labile host ranking in this pest species.La relación entre preferencia y rendimiento es crucial para la ecología y evolución de las interacciones insecto-planta. Se evaluó la preferencia de puesta y el rendimiento de la descendencia para una plaga de cítricos, el minador de la hoja Phyllocnistis citrella (Lepidoptera: Gracillariidae), sobre tres de sus plantas hospederas: limonero (Citrus limon [Linn.] Burm.), naranjo (Citrus sinensis [Linn.] Osbeck) y pomelo (Citrus paradisi Macfadyen) en la provincia de Tucumán (Noroeste de Argentina). Se realizaron pruebas con y sin elección en ambientes abiertos y cerrados, y se estimaron parámetros de rendimiento (tiempo de desarrollo, supervivencia, tamaño pupal y proporción de sexos) a partir de individuos criados en laboratorio y datos de tres años de muestreos de campo. Las tasas de parasitismo se estudiaron en pruebas de laboratorio con elección y evaluaciones en campo. Las tendencias de preferencia fueron inconsistentes, con limonero recibiendo más huevos en algunas pruebas, mientras que no se observó preferencia en otras. Los patrones de uso de hospedero en campo no mostraron diferencias significativas entre especies. El rendimiento del minador, incluyendo las tasas de parasitismo y depredación, fue generalmente homogéneo entre plantas hospederas. Con estos resultados, las plantas de limonero, naranjo y pomelo representarían intrínsecamente recursos similares para las poblaciones del minador de los cítricos en el NO de Argentina, tendencia que fue acompañada por la ausencia de preferencias consistentes en la puesta de huevos por parte de las hembras. Las condiciones ecológicas podrían ser más importantes que la adaptación fisiológica al moldear un rango de hospederos probablemente voluble en esta especie plaga.Fil: Goane, Lucía. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; ArgentinaFil: Valladares, Graciela Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Willink, Eduardo. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentin

An econometric analysis of tourism in Spain: implications for the sectoral study of exports and some economic policy considerations

This paper deals with the construction of econometric models to explain the external demand for Spanish tourist services. The models include as explanatory economic variables a tourist income index and two real exchange rate indices, one with respect to client countries and the other with respect to competitor countries. The results obtained : a) support the hypothesis that the decision to expend on tourism is made in two stages with different price and income elasticities in each of them; b) show that the recent drop in demand is due to a -real exchange rate effect. With the models constructed it is possible to evaluate to what extent the drop in demand is due purely to the effect of prices and to 'what extent it is determined by exchange rate movements. The latter have had an important effect, so that a policy of appreciating the peseta indeed has different sectoral effects. This different sectoral effect, as well as the importance of distinguishing between client countries and competitors, suggest that economic policy must not be based on just one index of the effective exchange rate of the peseta but on several

Safety & Contamination Information to Retailers regarding Control of Quality and Safety in Organic Production Chains

This leaflet provides a practical overview for retailers of what is done to secure the safety and purity of 7 types of organically pro-duced foods, and what the retailers can do to support those efforts and preserve the food safety until purchase. Other leaflets for re-tailers cover authenticity and fraud or taste, freshness & nutrients, and separate leaflets aim at consumers or at production of specific commodities

Safety and Contamination Information to Consumers regarding Control of Quality and Safety in Organic Production Chains

This leaflet provides a practical overview for consumers of what is done to secure the safety and purity of 7 types of organically pro-duced foods, and what the consumer can do to support those ef-forts and preserve the food safety after purchase. Other leaflets for consumers cover authenticity and fraud or taste, freshness & nu-trients, and separate leaflets aim at retailers or at production of specific commodities

Discerning the relationship between geminiviral infection and vesicle trafficking using virus induced gene silencing

Tomato yellow leaf curl disease is one of the most important threats to tomato crops worldwide. One of its causal agents, Tomato yellow leaf curl Sardinian virus (TYLCSV) is a monopartite member of the genus Begomovirus from the family Geminiviridae. Due to the few proteins encoded by their viral genome, geminiviruses rely heavily on host cellular machineries and interact with a wide range of plant proteins to complete all processes required for infection, such as viral replication, movement and suppression or evasion of plant defence mechanisms. The identification of the host proteins involved in viral infection will be an important step towards the understanding of the mechanisms underlying this process. In our laboratory, transgenic Nicotiana benthamiana plants containing a green fluorescent protein (GFP) expression cassette flanked by two direct repeats of the intergenic region of TYLCSV have been constructed (2IR plants). When these plants are infected with TYLCSV, an overexpression of the reporter gene is observed in those cells where the virus is actively replicating. These plants have been used together with virus induced gene silencing (VIGS) in an effort to identify host genes involved in the infection process using a reverse genetics approach. Using this combined technique our group has identified two genes δ-COP and ARF 1, involved in retrograde vesicle trafficking, which are essential for the infectious process. We are currently assaying genes codifying proteins involved in different pathways of the vesicle trafficking system: Sar1b, γ subunit of AP1, Sec24, SYT1 and two that encode the heavy chain of triskelion proteins. Their effect over viral infection will be presented and discussedUniversidad de Málaga. Campues de Excelencia Internacional Andalucía Tech

Identifying the function of vesicle trafficking in geminiviral infection using virus induced gene silencing

Tomato yellow leaf curl Sardinian virus (TYLCSV) is one of the causal agent of the tomato yel-low leaf curl disease, one of the most important threats to tomato crops worldwide. TYLCSV is a monopartite member of the genus Begomovirus from the family Geminiviridae. To carry out a full infection, geminiviruses need to move inside the infected cell and from one cell to an-other for which they depend on diverse cellular factors. While cell-to-cell movement has been described to occur through plasmodesmata, the way in which geminiviruses move inside the host cells is yet unknown. The identification of the host proteins involved in viral infection will be an important step to-wards the understanding of the mechanisms underlying this process. In our laboratory, trans-genic Nicotianabenthamiana plants containing a green fluorescent protein (GFP) expression cassette flanked by two direct repeats of the intergenic region of TYLCSV have been construct-ed (2IR plants). When these plants are infected with TYLCSV, an overexpression of the reporter gene is observed in those cells where the virus replicates. These plants have been used to-gether with virus induced gene silencing (VIGS) in an effort to identify host genes involved in the infection process using a reverse genetics approach. Using this combined technique our group has identified two genes δ-COP and ARF 1, involved in retrograde vesicle trafficking, which are essential for the infectious process. We are current-ly assaying genes codifying proteins involved in different pathways of the vesicle trafficking system: Sar1b, γ subunit of AP1, Sec24, SYT1 and two that encode the heavy chain of triskelion proteins. Their effect over virus infection will be presented and discussed.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Study and analysis of the use of flexibility in local electricity markets

In this work an introduction to Local Electricity Markets (LEM) was done and afterwards evolutionary algorithms (EAs) such as Differential Evolution (DE), HybridAdaptive Differential Evolution (HyDE), Hybrid-Adaptive Differential Evolution with Decay Function (HyDE-DF) and Vortex Search (VS) were applied to a market model in order to test its efficiency and scalability. Then, the market model was expanded adding a network model from the BISITE laboratory and again tests using the evolutionary algorithms were performed. In more detail, first a literature review is done about distributed generation, load flexibility, LEM and EAs. Then a cost optimization problem in Local Electricity Markets is analyzed considering fixed-term flexibility contracts between the distribution system operator (DSO) and aggregators. In this market structure, the DSO procures flexibility while aggregators of different types (e.g., conventional demand response or thermo-load aggregators) offer the service. Its then solved the proposed model using evolutionary algorithms based on the well-known differential evolution (DE). First, a parameter-tuning analysis is done to assess the impact of the DE parameters on the quality of solutions to the problem. Later, after finding the best set of parameters for the “tuned” DE strategies, we compare their performance with other self-adaptive parameter algorithms, namely the HyDE, HyDE-DF, and VS. Overall, the algorithms are able to find near-optimal solutions to the problem and can be considered an alternative solver for more complex instances of the model. After this a network model, from BISITE laboratory, is added to the problem and new analyses are performed using evolutionary algorithms along with MATPOWER power flow algorithms. Results show that evolutionary algorithms support from simple to complex problems, that is, it is a scalable algorithm, and with these results it is possible to perform analyses of the proposed market model.Neste trabalho foi feita uma introdução aos Mercados Locais de Eletricidade (MLE) e posteriormente foram aplicados algoritmos evolutivos (AEs) como Differential Evolution (DE), Hybrid-Adaptive Differential Evolution (HyDE), Hybrid-Adaptive Differential Evolution with Decay Function (HyDE-DF) e Vortex Search (VS) a um modelo de mercado a fim de testar a sua eficiência e escalabilidade. O modelo de mercado foi expandido adicionando uma rede do laboratório BISITE e novamente foram realizados testes usando os algoritmos evolutivos. Em mais detalhe, no trabalho primeiro foi feita uma revisão bibliográfica sobre geração distribuída, flexibilidade de carga, MLE e AEs. É analisado um problema de optimização de custos nos MLE, considerando contratos de flexibilidade a prazo fixo entre os agentes. O distribuidor adquire flexibilidade enquanto que os agregadores de diferentes tipos (por exemplo, os agregadores convencionais de resposta à procura ou de carga térmica) oferecem o serviço. Resolve-se depois o modelo proposto utilizando AEs baseados na conhecida DE. É feita uma análise de afinação de parâmetros para avaliar o impacto dos parâmetros DE na qualidade das soluções para o problema. Após encontrarmos o melhor conjunto de parâmetros para as estratégias DE "afinadas", comparamos o seu desempenho com outros algoritmos de parâmetros autoadaptáveis, nomeadamente o HyDE, HyDE-DF, e VS. Globalmente, os algoritmos são capazes de encontrar soluções quase óptimas para o problema e podem ser considerados um solucionador alternativo para instâncias mais complexas do modelo. Então um modelo de rede, do laboratório BISITE, é acrescentado ao problema e novas análises são realizadas utilizando algoritmos evolutivos juntamente com algoritmos de fluxo de potência MATPOWER. Os resultados mostram que os algoritmos evolutivos suportam desde problemas simples a complexos, ou seja, é um algoritmo escalável, e com estes resultados é possível realizar análises do modelo de mercado proposto