Bottom-Up Cubosome Synthesis Without Organic Solvents

This dataset contains processed SAXS data for mixtures of phytantriol with different diluents, and also following bottom-up synthesis of cubosomes. This data was collected to demonstrate the phase formations of phytantriol under different conditions.SAXS data was collected on an Anton-Parr SAXSpoint 2.0.SAXS data was collected on an Anton-Parr SAXSpoint 2.0, using an SDD of 556.9mm. The instrument is known to have a single dead pixel which sometimes results in an anomolous single-point peak

Evaluating the demand side: New challenges for evaluation

Evaluation of research and innovation policy faces radical challenges arising from a new policy emphasis upon demand-side measures and linked to this an understanding of innovation policy as a means to achieve societal goals. This article considers the implications for the practice of evaluation at both micro and meso-levels. It uses the exemplar of an evaluation design for the European Union's Lead Market Initiative to expose the extent to which classical approaches to evaluation are valid and where new issues arise. Some problems highlighted include the difficulty of establishing a relevant baseline, the inability of public statistics constructed in supply-side mode to capture actions, the need to engage with actors who do not necessarily see themselves as part of the initiative being evaluated, long timescales and potential wide geographical scope, measures that span from micro to macro, and blurred boundaries between implementation and impact. It is concluded that there is a key role for evaluators to become involved in co-learning and co-evolution of these policy instruments in a manner analogous to the relationship between evaluation and policy development that characterized the emergence of collaborative R&D support programmes

Mechanisms Regulating the Association of Protein Phosphatase 1 with Spinophilin and Neurabin

Protein phosphorylation is a key mediator of signal transduction, allowing for dynamic regulation of substrate activity. Whereas protein kinases obtain substrate specificity by targeting specific amino acid sequences, serine/threonine phosphatase catalytic subunits are much more promiscuous in their ability to dephosphorylate substrates. To obtain substrate specificity, serine/threonine phosphatases utilize targeting proteins to regulate phosphatase subcellular localization and catalytic activity. Spinophilin and its homologue neurabin are two of the most abundant dendritic spine-localized protein phosphatase 1 (PP1) targeting proteins. The association between spinophilin and PP1 is increased in the striatum of animal models of Parkinson's disease (PD). However, mechanisms that regulate the association of spinophilin and neurabin with PP1 are unclear. Here, we report that the association between spinophilin and PP1α or PP1γ1 was increased by CDK5 expression and activation in a heterologous cell system. This increased association is at least partially due to phosphorylation of PP1. Conversely, CDK5 expression and activation decreased the association of PP1 with neurabin. As with dopamine depletion, methamphetamine (METH) abuse causes persistent alterations in dopamine signaling which influence striatal medium spiny neuron function and biochemistry. Moreover, both METH toxicity and dopamine depletion are associated with deficits in motor control and motor learning. Pathologically, we observed a decreased association of spinophilin with PP1 in rat striatum evaluated one month following a binge METH paradigm. Behaviorally, we found that loss of spinophilin recapitulates rotarod pathology previously observed in dopamine-depleted and METH-treated animals. Together, these data have implications in multiple disease states associated with altered dopamine signaling such as PD and psychostimulant drug abuse and delineate a novel mechanism by which PP1 interactions with spinophilin and neurabin may be differentially regulated

Adolescent Perceptions of Injury and Pressures of Returning to Sport: A Retrospective Qualitative Analysis

The increase in sport participation among adolescents has led to the rise in sport-related injuries, many of which have unique characteristics based on the patient, their perceptions, and the pressures faced when returning to sport. The purpose of this study was to identify the underlying factors that contributed to adolescents’ perceptions of injury and the various pressures they experienced when returning to sport. Two themes emerged from the study: support and fear. Support was provided to participants through development, care, and the environment. Fear was the factor that affected the participant in their return to sport, which came in the form of worry and doubt

Voltammetric optimisation of TEMPO-mediated oxidations at cellulose fabric

A simple method for rapid voltammetric screening of N-oxyl based mediators and reaction conditions for electrochemical oxidation of cellulose is described. Using immobilised cellulose (cotton cloth) the screening requires small quantities of mediator and yet yields information about relative efficiency of mediator, optimum pH, and preferred buffer identity.</p

Surfactant behavior of sodium dodecylsulfate in deep eutectic solvent choline chloride/urea

Deep eutectic solvents (DES) resemble ionic liquids but are formed from an ionic mixture instead of being a single ionic compound. Here we present some results that demonstrate that surfactant sodium dodecyl sulfate (SDS) remains surface-active and shows self-assembly phenomena in the most commonly studied DES, choline chloride/urea. X-ray reflectivity (XRR) and small angle neutron scattering (SANS) suggest that the behavior is significantly different from that in water. Our SANS data supports our determination of the critical micelle concentration using surface-tension measurements and suggests that the micelles formed in DES do not have the same shape and size as those seen in water. Reflectivity measurements have also demonstrated that the surfactants remain surface-active below this concentration