Identificação de polinizadores na progênie da matriz de erva-mate cambona-4, usando marcadores RAPD.

O presente trabalho teve como objetivo determinar a paternidade de uma progênie de erva-mate da matriz Cambona-4 e quatro possíveis polinizadores (A,B,C e D). O mesmo visa a produção de mudas por sementes com a manutenção das características agronômicas e principalmente suavidade no sabor, inerentes à matriz Cambona-4. Para tanto, foram utilizados marcadores RAPD identificando-se fragmentos presentes em apenas um dos polinizadores e na progênie e, obrigatoriamente, ausentes nos demais polinizadores e na matriz. Um mínimo de seis fragmentos com estas características foram usados para determinação de cada paternidade. Obteve-se a confirmação de 83,3% de filhos oriundos do cruzamento com o polinizador A, 11,9% com o polinizador B, 4,8% com o polinizador C. Nenhuma planta da progênie confirmou ser filha do cruzamento com o polinizador D. De um total de 107 progênies testadas, 23 não foram possíveis de determinar sua paternidade com os primers utilizados. A determinação da paternidade preferencialmente pelo polinizador A permitirá o direcionamento de cruzamentos a fim de obter-se sementes e mudas com uma variabilidade baixa e características próximas à da matriz Cambona-4, uma vez que as plantas da progênie confirmadas como Cambona-4/polinizador A apresentaram características próximas à planta matriz, com 90% das plantas semelhantes quanto à cor da folha e 84% quanto ao brilho da folha. Testes preliminares de degustação mostram que a característica de suavidade no sabor está mantida nesta progênie, embora ensaios sensoriais estejam em fase de programação.Secão: Conservação, Melhoramento e Multiplicação. Feira do Agronegócio da Erva-mate, 1., 2003, Chapecó. Integrar para promover o agronegócio da erva-mate

Colletotrichum species associated to ripe rot disease of grapes in the “Serra Gaucha” region of Southern Brazil

Ripe rot disease caused by Colletotrichum (Glomerella) has become a serious problem for viticulture in Southern Brazil. Global warming contributes to the increase of this devastating and difficult to control disease. Several species of Colletotrichum, with different phytopathological characteristics, have been associated with ripe rot disease in different viticultural regions. In this article, a total of 63 fungi were isolated from grapes showing symptoms of ripe rot disease, and classified by sequencing of ITS region, and compared with the sequences deposited in GenBank. The isolates were included in three clades of Colletotrichum: 84.1% belonged to the “gloeosporioides” clade, 3.2% to the “boninense” clade, and 12.7% to the “acutatum” clade. Of the 53 isolates included in the “gloeosporioides” clade, 44.4% were classified as C. viniferum/C. ampelinum, 37.1% as C. fruticola, 13.0% as C. kahawae and 5.5% as a species related to C. fruticola. In turn, the two isolates of the “boninense” clade were classified as C. kartii/C. phyllanthi, and the six “acutatum” isolates were similar to C. acutatum and C. nymphaeae reference materials. The identified species were previously linked to ripe rot disease in other viticulture regions of the world, but the frequency of some species in southern Brazil is particularly different

Chemical composition and sensory evaluation of wines produced with different Moscato varieties

Moscato grapes give very aromatic musts and wines, characterized by the presence of monoterpenoids. The Farroupilha region situated in the highlands of South Brazil, is responsible for 50% of Brazilian Moscato wines production, and obtained its Geographical Indication (GI) in 2015. Brazilian Moscato wines are produced with several varieties, mainly Moscato Branco, Moscato Bianco R2 and Moscato Giallo. The objective of this study was to characterize the aromatic profile of wines produced with the three varieties. Microvinifications were conducted with grapes collected in three vineyards located in Farroupilha. Volatile compounds were evaluated using gas chromatography, and sensory characteristics were determined by a panel of enologists using a specific descriptive chard. Moscato Giallo wines exhibited the highest concentrations of ethyl acetate, 2-phenylethanol, isoamyl acetate, linalool, and α-terpineol; Moscato R2 wines showed the highest concentrations of ethyl decanoate and nerol, while Moscato Branco wines, the most representative variety of Brazilian sparkling Moscato wines, exhibited the highest concentrations of ethanal and ethyl hexanoate, and intermediary concentrations of the other compounds. In sensory analysis, the three varieties exhibited pear, pitanga, rosemary, and citric fruits aromas, but in general, Moscato Giallo and Moscato R2 were more intense, while Moscato Branco showed the highest acidity

Nanoindentation unidirectional sliding and lateral force microscopy: evaluation of experimental techniques to measure friction at the nanoscale

CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPERG - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DO RIO GRANDE DO SULFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOLateral force microscopy (LFM) is an established technique to assess friction forces at the nanoscale. Nanoindentation followed by unidirectional sliding (NUS) is also used to evaluate friction forces at the micro/nanoscale. However, comparative studies between NUS and LFM evaluating the experimental results at different scales are still missing. In this work, a-C:D/H and a-C:H thin films with different [D]/[C] and [H]/[C] contents were used to analyze the friction forces by NUS and LFM. The results show that the friction behavior assessed by these two techniques in different scales is the same. The correlation between friction forces measured by NUS and LFM depends mainly on a contact area factor that makes invariant the friction force from nanoscale to microscale. Such behavior suggests a similar damping mechanism, probably phonon-coupling phenomena, for the friction force origin.812110CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPERG - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DO RIO GRANDE DO SULFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPERG - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DO RIO GRANDE DO SULFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO465423/2014-0sem informação2012/10127-

Rules extraction from neural networks applied to the prediction and recognition of prokaryotic promoters

Promoters are DNA sequences located upstream of the gene region and play a central role in gene expression. Computational techniques show good accuracy in gene prediction but are less successful in predicting promoters, primarily because of the high number of false positives that reflect characteristics of the promoter sequences. Many machine learning methods have been used to address this issue. Neural Networks (NN) have been successfully used in this field because of their ability to recognize imprecise and incomplete patterns characteristic of promoter sequences. In this paper, NN was used to predict and recognize promoter sequences in two data sets: (i) one based on nucleotide sequence information and (ii) another based on stability sequence information. The accuracy was approximately 80% for simulation (i) and 68% for simulation (ii). In the rules extracted, biological consensus motifs were important parts of the NN learning process in both simulations