Phenotypic robustness can increase phenotypic variability after non-genetic perturbations in gene regulatory circuits

Non-genetic perturbations, such as environmental change or developmental noise, can induce novel phenotypes. If an induced phenotype confers a fitness advantage, selection may promote its genetic stabilization. Non-genetic perturbations can thus initiate evolutionary innovation. Genetic variation that is not usually phenotypically visible may play an important role in this process. Populations under stabilizing selection on a phenotype that is robust to mutations can accumulate such variation. After non-genetic perturbations, this variation can become a source of new phenotypes. We here study the relationship between a phenotype's robustness to mutations and a population's potential to generate novel phenotypic variation. To this end, we use a well-studied model of transcriptional regulation circuits. Such circuits are important in many evolutionary innovations. We find that phenotypic robustness promotes phenotypic variability in response to non-genetic perturbations, but not in response to mutation. Our work suggests that non-genetic perturbations may initiate innovation more frequently in mutationally robust gene expression traits.Comment: 11 pages, 5 figure

Lung Nodules in Melanoma Patients: Morphologic Criteria to Differentiate Non-Metastatic and Metastatic Lesions

Lung nodules are frequent findings in chest computed tomography (CT) in patients with metastatic melanoma. In this study, we assessed the frequency and compared morphologic differences of metastases and benign nodules. We retrospectively evaluated 85 patients with melanoma (AJCC stage III or IV). Inclusion criteria were ≤20 lung nodules and follow-up using CT ≥183 days after baseline. Lung nodules were evaluated for size and morphology. Nodules with significant growth, nodule regression in line with RECIST assessment or histologic confirmation were judged to be metastases. A total of 438 lung nodules were evaluated, of which 68% were metastases. At least one metastasis was found in 78% of patients. A 10 mm diameter cut-off (used for RECIST) showed a specificity of 95% and a sensitivity of 20% for diagnosing metastases. Central location (n = 122) was more common in metastatic nodules (p = 0.009). Subsolid morphology (n = 53) was more frequent (p < 0.001), and calcifications (n = 13) were solely found in non-metastatic lung nodules (p < 0.001). Our data show that lung nodules are prevalent in about two-thirds of melanoma patients (AJCC stage III/IV) and the majority are metastases. Even though we found a few morphologic indicators for metastatic or non-metastatic lung nodules, morphology has limited value to predict the presence of lung metastases

Conclusive quantum steering with superconducting transition edge sensors

Quantum steering allows two parties to verify shared entanglement even if one measurement device is untrusted. A conclusive demonstration of steering through the violation of a steering inequality is of considerable fundamental interest and opens up applications in quantum communication. To date all experimental tests with single photon states have relied on post-selection, allowing untrusted devices to cheat by hiding unfavourable events in losses. Here we close this "detection loophole" by combining a highly efficient source of entangled photon pairs with superconducting transition edge sensors. We achieve an unprecedented ~62% conditional detection efficiency of entangled photons and violate a steering inequality with the minimal number of measurement settings by 48 standard deviations. Our results provide a clear path to practical applications of steering and to a photonic loophole-free Bell test.Comment: Preprint of 7 pages, 3 figures; the definitive version is published in Nature Communications, see below. Also, see related experimental work by A. J. Bennet et al., arXiv:1111.0739 and B. Wittmann et al., arXiv:1111.076

Performance Analysis of High Temperature Heat Pump Cycle for Industrial Process

High-temperature heat pumps (HTHP) that can supply heat at temperatures above 200 °C have a very large potential to enhance the energy efficiency of the industrial sector and reduce its CO2 emissions. In the current work, the thermodynamic performance of three different vapor compression cycles, which use R-718 (water) as a working medium, have been evaluated using a commercial process simulation tool (EBSILON Professional 15). All considered cycles use two-stage vapor compression with different intercooling strategies between the stages and the thermodynamic performances of those cycles have been investigated. The comparison studies on the coefficient of performance (COP) and heat supply temperatures have been conducted for all cycle architectures. With increasing temperature difference between a heat source and heat sink, ∆T, the COP values decreased as expected. The highest COP value was found for the cycle configurations where both compressors have the same pressure ratio (PR). The investigation on the HTHP capacities and exergy efficiency, ηexergy, with optimized PR has also been carried out. Both COP and ηexergy values increased with decreasing ∆T and the intercooler cycles with heat exchanger showed higher COP and ηexergy than the spray injection cycle

Criteria for the use of omics-based predictors in clinical trials.

The US National Cancer Institute (NCI), in collaboration with scientists representing multiple areas of expertise relevant to 'omics'-based test development, has developed a checklist of criteria that can be used to determine the readiness of omics-based tests for guiding patient care in clinical trials. The checklist criteria cover issues relating to specimens, assays, mathematical modelling, clinical trial design, and ethical, legal and regulatory aspects. Funding bodies and journals are encouraged to consider the checklist, which they may find useful for assessing study quality and evidence strength. The checklist will be used to evaluate proposals for NCI-sponsored clinical trials in which omics tests will be used to guide therapy

Chiral dynamics of p-wave in K^- p and coupled states

We perform an evaluation of the p-wave amplitudes of meson-baryon scattering in the strangeness S=-1 sector starting from the lowest order chiral Lagrangians and introducing explicitly the Sigma^* field with couplings to the meson-baryon states obtained using SU(6) symmetry. The N/D method of unitarization is used, equivalent, in practice, to the use of the Bethe-Salpeter equation with a cut-off. The procedure leaves no freedom for the p-waves once the s-waves are fixed and thus one obtains genuine predictions for the p-wave scattering amplitudes, which are in good agreement with experimental results for differential cross sections, as well as for the width and partial decay widths of the Sigma^*(1385).Comment: LaTeX, 18 pages, 6 figure

Bell inequalities for entangled kaons and their unitary time evolution

We investigate Bell inequalities for neutral kaon systems from Phi resonance decay to test local realism versus quantum mechanics. We emphasize the unitary time evolution of the states, that means we also include all decay product states, in contrast to other authors. Only this guarantees the use of the complete Hilbert space. We develop a general formalism for Bell inequalities including both arbitrary "quasi spin" states and different times; finally we analyze Wigner-type inequalities. They contain an additional term, a correction function h, as compared to the spin 1/2 or photon case, which changes considerably the possibility of quantum mechanics to violate the Bell inequality. Examples for special "quasi spin" states are given, especially those which are sensitive to the CP parameters epsilon and epsilon'.Comment: REVTeX, 22 page