Detours are the straight way to success on the Internet (Matt Locke at Polis summer school – guestblog)

How do you catch the attention of young people in a world, where the amount of information available is so massive, indeed,, for all practical reasons, infinite? That is the question Matt Locke, commissioning editor on Channel 4, is facing everyday, as he works with the channel’s section for teenagers, producing content about the choices you face as a teenager, trying to grasp the world of grown-ups, that they are about to enter

Dissection of the antimicrobial and hemolytic activity of Cap18: Generation of Cap18 derivatives with enhanced specificity

<div><p>Due to the rapid emergence of resistance to classical antibiotics, novel antimicrobial compounds are needed. It is desirable to selectively kill pathogenic bacteria without targeting other beneficial bacteria in order to prevent the negative clinical consequences caused by many broad-spectrum antibiotics as well as reducing the development of antibiotic resistance. Antimicrobial peptides (AMPs) represent an alternative to classical antibiotics and it has been previously demonstrated that Cap18 has high antimicrobial activity against a broad range of bacterial species. In this study we report the design of a positional scanning library consisting of 696 Cap18 derivatives and the subsequent screening for antimicrobial activity against <i>Y</i>. <i>ruckeri</i>, <i>A</i>. <i>salmonicida</i>, <i>S</i>. Typhimurium and <i>L</i>. <i>lactis</i> as well as for hemolytic activity measuring the hemoglobin release of horse erythrocytes. We show that the hydrophobic face of Cap18, in particular I13, L17 and I24, is essential for its antimicrobial activity against <i>S</i>. Typhimurium, <i>Y</i>. <i>ruckeri</i>, <i>A</i>. <i>salmonicida</i>, <i>E</i>. <i>coli</i>, <i>P</i>. <i>aeruginosa</i>, <i>L</i>. <i>lactis</i>, <i>L</i>. <i>monocytogenes</i> and <i>E</i>. <i>faecalis</i>. In particular, Cap18 derivatives harboring a I13D, L17D, L17P, I24D or I24N substitution lost their antimicrobial activity against any of the tested bacterial strains. In addition, we were able to generate species-specific Cap18 derivatives by particular amino acid substitutions either in the hydrophobic face at positions L6, L17, I20, and I27, or in the hydrophilic face at positions K16 and K18. Finally, our data showed the proline residue at position 29 to be essential for the inherent low hemolytic activity of Cap18 and that substitution of the residues K16, K23, or G21 by any hydrophobic residues enhances the hemolytic activity. This study demonstrates the potential of generating species-specific AMPs for the selective elimination of bacterial pathogens.</p></div

Architectures of disavowal and enduring acts of refusal

Engaging with scholarship on vertical urbanism, this paper advances an understanding of architectural disavowal to account for the ways that vertical architectures deny their responsibility in causing harm to residential populations. The paper draws on Dionne Brand’s notion of disavowal in the vertical city to examine residents’ experience of and resistant responses to the harmful effects that vertical developments impose on their daily and nightly lives. In Aldgate, east London, the 13-storey high-rise development, Buckle Street Studio, has caused noise levels to rise, light pollution to intensify, the sky to vanish from sight and daylight to disappear from the flats in the neighbouring block Goldpence Apartments. Drawing on interviews with residents in Goldpence Apartments, the paper documents the extent of these changes and brings attention to the mundane strategies that residents deploy in their attempts to resist the overwhelming sensory invasions and affective intrusions of their homes. By showcasing how residents overturn the affective charge of their new high-rise neighbour/s and refuse – disavow – its force, the paper considers how mundane survival strategies might challenge architectural disavowal in the vertical city and beyond

Rethinking urban lighting: geographies of artificial lighting in everyday life

PhDIn this thesis I study the role of artificial lighting in the everyday urban life of older residents living in the London Borough of Newham. Newham’s light infrastructure is currently undergoing change as the borough’s entire 19,000 street lamps are being re-placed with Light Emitting Diodes and as a range of regeneration projects provide public spaces designed with new lighting. By increasing visibility and encouraging everyday activity into the evening, the Council claims that the changes in public light-ing will provide ‘eyes on the streets’ and encourage ‘eyes from the windows’ of build-ings, contributing to increasing ‘natural surveillance’. The Council’s avowal of every-day practices in streets and in homes, has made me question how lighting affects the way older residents move through streets and carry out domestic practices as dark-ness falls. The study explores how light planning, lighting design and everyday, rou-tine practices in the public realm and inside homes co-produce the urban, lit environ-ment. Two major contributions of the thesis lie in the (post)phenomenological ap-proach I develop to study everyday experiences of urban lighting, and the methodo-logical framework I employ to research such practices, which combines mobile and visual methods. I have conducted 11 in-depth interviews with nine different planners and designers, 12 walk-along interviews with 22 residents between 58 and 79 years old, and a collaborative photography project with 14 residents between 68 and 96 years old. As I show how older residents experiences different lighting technologies, layers of light, and different lit spaces in their neighbourhoods, I discuss how urban lighting makes them see, feel and carry out routine practices in particular ways. Based on my findings, I argue that urban lighting shapes what, and how, people see, but how people see depends on how they negotiate changes in lighting. In a range of examples where residents mould the urban, lit environment or respond to lighting in different ways, I show how they play and active part in co-producing ways of seeing. I argue it is crucial that light planners and lighting designers recognise such co-constitutive role of everyday practices in order to ensure better lighting for our future cities

Light violence at the threshold of acceptability

This paper shows how residential high-rise developments in London deteriorate the living conditions for existing residents and set a legal precedent for distributing harm unevenly across the population. The paper unpacks the contentious decision-making process in one of several local planning applications in the London Borough of Tower Hamlets that ended in a spur of high-profile public planning inquiries between 2017 and 2019. The Enterprise House inquiry shows how, among other things, a loss of daylight, sunlight and outlook, and an increased sense of enclosure, affect already marginalised residents in neighbouring buildings disproportionately, elevating light to a legal category for assessing harm and addressing social injustice in the vertical city. The paper adopts a forensic approach to interrogate four instances during the public inquiry, in which numerical evidence of material harm resulting from a loss in daylight, sunlight and outlook was made to appear and disappear. The translation of scientific evidence into legal evidence is performed through the act of claiming ‘truthful’ representations of ‘real life experiences’ of light in digital visualisations. By revealing how material harm resulting from vertical development is normalised and thus naturalised in the planning inquiry, the paper demonstrates how ‘light’ violence is exercised in vertical development