A Short-Term Assessment of Carbon Dioxide Fluxes under Contrasting Agricultural and Soil Management Practices in Zimbabwe

Two of the biggest problems facing humankind are feeding an exponentially growing human population and preventing the accumulation of atmospheric greenhouse gases and its climate change consequences. Refined agricultural practices could address both of these problems. The research addressed here is an exploration of the efficacy of alternative agricultural practices in sequestering carbon (C). The study was conducted in Zimbabwe with the intent to (a) demonstrate the utility of micrometeorological methods for measuring carbon dioxide (CO2) exchange between the surface and the atmosphere in the short-term, and (b) to quantify differences in such exchange rates for a variety of agricultural practices. Four Bowen ratio energy balance (BREB) systems were established on the following agricultural management practices: (1) no-till (NT) followed by planting of winter wheat (Triticum aestivum), (2) NT followed by planting of blue lupin (Lupinus angustifolios L.), (3) maize crop residue (Zea mays L.) left on the surface, and (4) maize crop residue incorporated with tillage. Over a period of 139 days (from 15 June to 31 October 2013) the winter wheat cover crop produced a net accumulation of 257 g CO2-C m-2, while the tilled plot with no cover crop produced a net emission of 197 g CO2-C m-2 and the untilled plot with no cover emitted 235 g CO2-C m-2. The blue lupin cover crop emitted 58 g CO2-C m-2, indicating that winter cover crops can sequester carbon and reduce emissions over land left fallow through the non-growing season. The micrometeorological methods described in this work can detect significant differences between treatments over a period of a few months, an outcome important to determine which smallholder soil management practices can contribute towards mitigating climate change

Nutrient Source and Tillage Effects on Maize: II. Yield, Soil Carbon, and Carbon Dioxide Emissions

There is a need to understand the potential benefits of using the biotechnology waste by‐product from manufacturing as a fertilizer replacement in agriculture, by quantifying the economic value for the farmer and measuring the environmental impact. Measuring CO2 emissions can be used to assess environmental impact, including three widely used micrometeorological methodologies: (i) the Bowen Ratio Energy Balance (BREB), (ii) aerodynamic flux‐gradient theory, and (iii) eddy covariance (EC). As a first step in quantifying benefits of applying biotechnology waste in agriculture, a detailed examination of these three methods was conducted to understand their effectiveness in quantifying CO2 emissions for this specific circumstance. The study measured micrometeorological properties over a field planted to maize (Zea mays L. var. indentata ), one plot treated with biotechnology waste applied as a nutrient amendment, and one plot treated with a typical farmer fertilizer practice. Carbon dioxide flux measurements took place over 1 yr, using both BREB and EC systems. The aerodynamic method was used to gap‐fill BREB system measurements, and those flux estimates were compared with estimates produced separately by the aerodynamic and EC methods. All methods found greater emissions over the biotechnology waste application. The aerodynamic method CO2 flux estimates were considerably greater than both the EC and a combined BREB‐aerodynamic approach. During the day, the EC and BREB methods agree. At night, the aerodynamic approach detects and accounts for buildup of CO2 at the surface during stable periods. The BREB systems combined with aerodynamic approaches provide alternate methods to EC in examining micrometeorological properties near the surface

Nutrient Source and Tillage Effects on Maize: I. Micrometeorological Methods for Measuring Carbon Dioxide Emissions

There is a need to understand the potential benefits of using the biotechnology waste by‐product from manufacturing as a fertilizer replacement in agriculture, by quantifying the economic value for the farmer and measuring the environmental impact. Measuring CO2 emissions can be used to assess environmental impact, including three widely used micrometeorological methodologies: (i) the Bowen Ratio Energy Balance (BREB), (ii) aerodynamic flux‐gradient theory, and (iii) eddy covariance (EC). As a first step in quantifying benefits of applying biotechnology waste in agriculture, a detailed examination of these three methods was conducted to understand their effectiveness in quantifying CO2 emissions for this specific circumstance. The study measured micrometeorological properties over a field planted to maize (Zea mays L. var. indentata ), one plot treated with biotechnology waste applied as a nutrient amendment, and one plot treated with a typical farmer fertilizer practice. Carbon dioxide flux measurements took place over 1 yr, using both BREB and EC systems. The aerodynamic method was used to gap‐fill BREB system measurements, and those flux estimates were compared with estimates produced separately by the aerodynamic and EC methods. All methods found greater emissions over the biotechnology waste application. The aerodynamic method CO2 flux estimates were considerably greater than both the EC and a combined BREB‐aerodynamic approach. During the day, the EC and BREB methods agree. At night, the aerodynamic approach detects and accounts for buildup of CO2 at the surface during stable periods. The BREB systems combined with aerodynamic approaches provide alternate methods to EC in examining micrometeorological properties near the surface

Conservation agriculture as a climate change mitigation strategy in Zimbabwe

There is a need to quantify agriculture’s potential to sequester carbon (C) to inform global approaches aimed at mitigating climate change effects. Many factors including climate, crop, soil management practices, and soil type can influence the contribution of agriculture to the global carbon cycle. The objective of this study was to investigate the C sequestration potential of conservation agriculture (CA) (defined by minimal soil disturbance, maintaining permanent soil cover, and crop rotations). This study used micrometeorological methods to measure carbon dioxide (CO2) flux from several alternative CA practices in Harare, central Zimbabwe. Micrometeorological methods can detect differences in total CO2 emissions of agricultural management practices; our results show that CA practices produce less CO2 emissions. Over three years of measurement, the mean and standard error (SE) of CO2 emissions for the plot with the most consistent CA practices was 0.564 ± 0.0122 g CO2 m-2 h-1, significantly less than 0.928 ± 0.00859 g CO2 m-2 h-1 for the conventional tillage practice. Overall CA practices of no-till with the use of cover crops produced fewer CO2 emissions than conventional tillage or fallow

Bowen Ratio Energy Balance Measurement of Carbon Dioxide (CO2) Fluxes of No-Till and Conventional Tillage Agriculture in Lesotho

Global food demand requires that soils be used intensively for agriculture, but how these soils are managed greatly impacts soil fluxes of carbon dioxide (CO2). Soil management practices can cause carbon to be either sequestered or emitted, with corresponding uncertain influence on atmospheric CO2 concentrations. The situation is further complicated by the lack of CO2 flux measurements for African subsistence farms. For widespread application in remote areas, a simple experimental methodology is desired. As a first step, the present study investigated the use of Bowen Ratio Energy Balance (BREB) instrumentation to measure the energy balance and CO2 fluxes of two contrasting crop management systems, till and no-till, in the lowlands within the mountains of Lesotho. Two BREB micrometeorological systems were established on 100-m by 100-m sites, both planted with maize (Zea mays) but under either conventional (plow, disk-disk) or no-till soil mangement systems. The results demonstrate that with careful maintenance of the instruments by appropriately trained local personnel, the BREB approach offers substantial benefits in measuring real time changes in agroecosystem CO2 flux. The periods where the two treatments could be compared indicated greater CO2 sequestration over the no-till treatments during both the growing and non-growing seasons

Neutrophils in cancer: neutral no more

Neutrophils are indispensable antagonists of microbial infection and facilitators of wound healing. In the cancer setting, a newfound appreciation for neutrophils has come into view. The traditionally held belief that neutrophils are inert bystanders is being challenged by the recent literature. Emerging evidence indicates that tumours manipulate neutrophils, sometimes early in their differentiation process, to create diverse phenotypic and functional polarization states able to alter tumour behaviour. In this Review, we discuss the involvement of neutrophils in cancer initiation and progression, and their potential as clinical biomarkers and therapeutic targets

A Lipid Receptor Sorts Polyomavirus from the Endolysosome to the Endoplasmic Reticulum to Cause Infection

The mechanisms by which receptors guide intracellular virus transport are poorly characterized. The murine polyomavirus (Py) binds to the lipid receptor ganglioside GD1a and traffics to the endoplasmic reticulum (ER) where it enters the cytosol and then the nucleus to initiate infection. How Py reaches the ER is unclear. We show that Py is transported initially to the endolysosome where the low pH imparts a conformational change that enhances its subsequent ER-to-cytosol membrane penetration. GD1a stimulates not viral binding or entry, but rather sorting of Py from late endosomes and/or lysosomes to the ER, suggesting that GD1a binding is responsible for ER targeting. Consistent with this, an artificial particle coated with a GD1a antibody is transported to the ER. Our results provide a rationale for transport of Py through the endolysosome, demonstrate a novel endolysosome-to-ER transport pathway that is regulated by a lipid, and implicate ganglioside binding as a general ER targeting mechanism

Adaptive Mutations in the JC Virus Protein Capsid Are Associated with Progressive Multifocal Leukoencephalopathy (PML)

PML is a progressive and mostly fatal demyelinating disease caused by JC virus infection and destruction of infected oligodendrocytes in multiple brain foci of susceptible individuals. While JC virus is highly prevalent in the human population, PML is a rare disease that exclusively afflicts only a small percentage of immunocompromised individuals including those affected by HIV (AIDS) or immunosuppressive drugs. Viral- and/or host-specific factors, and not simply immune status, must be at play to account for the very large discrepancy between viral prevalence and low disease incidence. Here, we show that several amino acids on the surface of the JC virus capsid protein VP1 display accelerated evolution in viral sequences isolated from PML patients but not in sequences isolated from healthy subjects. We provide strong evidence that at least some of these mutations are involved in binding of sialic acid, a known receptor for the JC virus. Using statistical methods of molecular evolution, we performed a comprehensive analysis of JC virus VP1 sequences isolated from 55 PML patients and 253 sequences isolated from the urine of healthy individuals and found that a subset of amino acids found exclusively among PML VP1 sequences is acquired via adaptive evolution. By modeling of the 3-D structure of the JC virus capsid, we showed that these residues are located within the sialic acid binding site, a JC virus receptor for cell infection. Finally, we go on to demonstrate the involvement of some of these sites in receptor binding by demonstrating a profound reduction in hemagglutination properties of viral-like particles made of the VP1 protein carrying these mutations. Collectively, these results suggest that a more virulent PML causing phenotype of JC virus is acquired via adaptive evolution that changes viral specificity for its cellular receptor(s)

A Novel Role for CD55 in Granulocyte Homeostasis and Anti-Bacterial Host Defense

Background: In addition to its complement-regulating activity, CD55 is a ligand of the adhesion class G protein-coupled receptor CD97; however, the relevance of this interaction has remained elusive. We previously showed that mice lacking a functional CD97 gene have increased numbers of granulocytes. Methodology/Results: Here,wedemonstratethatCD55-deficientmicedisplay a comparable phenotype with about two-fold more circulating granulocytes in the blood stream, the marginated pool, and the spleen. This granulocytosis was independent of increased complement activity. Augmented numbers of Gr-1-positive cells in cell cycle in the bone marrow indicated a higher granulopoietic activity in mice lacking either CD55 or CD97. Concomitant with the increase in blood granulocyte numbers, Cd55-/mice challenged with the respiratory pathogen Streptococcus pneumoniae developed less bacteremia and died later after infection. Conclusions: Collectively, these data suggest that complement-independent interaction of CD55 with CD97 is functionall