Analysis of genetic structure of some native turkish goat breeds by 20 microsatellite markers

Genetic diversity, genetic relationship and bottleneck were evaluated in Angora, Kilis, Honamli, Hair and Norduz goat breeds using 20 microsatellite markers. Analyses revealed that the average number of alleles per locus (15.65 allele/locus) and levels of heterozygosity (0.5192–0.9400) were fairly high. The calculated overall FIS value for all populations was 0.03656 ± 0.033 and it was not significant. All the populations were in the Hardy–Weinberg equilibrium. Gene exchange among populations was consistently high, its rate being Nm = 8.07 migrants per generation. According to FST values, a medium level of genetic diversity was found between the Angora goat breed and other breeds. Among the other breeds, genetic diversity was low and this diversity was statistically significant. Results of various analyses, such as allelic variation analysis, heterozygosity analysis, F statistics, STRUCTURE test and factorial correspondence analysis, indicated that the Angora goat breed is different than the other goat breeds. Furthermore, analysis showed that the other native goat breeds could not be distinguished from each other; these breeds were grouped together. The results obtained from the analysis of 20 microsatellite loci indicated that goat breeds other than the Angora goat breed cannot be genetically distinguished from each other

Analysis of genetic structure of some native turkish goat breeds by 20 microsatellite markers

Genetic diversity, genetic relationship and bottleneck were evaluated in Angora, Kilis, Honamli, Hair and Norduz goat breeds using 20 microsatellite markers. Analyses revealed that the average number of alleles per locus (15.65 allele/locus) and levels of heterozygosity (0.5192–0.9400) were fairly high. The calculated overall FIS value for all populations was 0.03656 ± 0.033 and it was not significant. All the populations were in the Hardy–Weinberg equilibrium. Gene exchange among populations was consistently high, its rate being Nm = 8.07 migrants per generation. According to FST values, a medium level of genetic diversity was found between the Angora goat breed and other breeds. Among the other breeds, genetic diversity was low and this diversity was statistically significant. Results of various analyses, such as allelic variation analysis, heterozygosity analysis, F statistics, STRUCTURE test and factorial correspondence analysis, indicated that the Angora goat breed is different than the other goat breeds. Furthermore, analysis showed that the other native goat breeds could not be distinguished from each other; these breeds were grouped together. The results obtained from the analysis of 20 microsatellite loci indicated that goat breeds other than the Angora goat breed cannot be genetically distinguished from each other

Prolactin receptor (PRLR) gene polymorphism in Chios, White Karaman and Awassi sheep breeds

The objective of the present study was to determine the polymorphism in the prolactine receptor (PRLR) gene in Chios, White Karaman and Awassi, which are native sheep breeds in Turkey. By means of PRLR gene sequence homology between sheep and humans, two primer pairs were designed for polymerase chain reaction (PCR) amplification within intron 1 and exon 10 of the PRLR gene in sheep. A total of 160 amplicons (99 for intron 1 and 61 for exon 10) were subjected to DNA sequence analysis. For intron 1, 6 different haplotypes were determined. For exon 10, 7 different haplotypes were obtained. Some variations determined for exon 10 (g.14A>T p.Q14L; g.160G>A p.D160N; g.166G>A p.E166K; g.167A>T p.E167V; g.176A>T p.H176L; g.206G>A p.S206N; g.208G>A p.G208R) led to changes in the amino acids, but no amino acid changes were determined in g.2A>T, g.81A>G, g.138A>G, g.186C>T, g..207T>C. It was noted in particular that White Karaman and Awassi were similar to each other in both PRLR exon 10 and intron 1 haplotypes, whereas the Chios breed had a different variation.TubitakTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK); Council of Firat UniversityFirat University [1070894]The sequence experiments were carried out at the Ankara University Biotechnology Institute Genomics Unit. The Project was supported by Tubitak and Scientific Research Projects Council of Firat University (Project code Tubitak 1070894; Fubap: 1502)

Does biochemical profile of follicular fluid in women undergoing assisted reproduction vary between the right and left ovaries?

Background: Follicular fluid (FF) provides a substantial micro-milieu for the development of the oocytes. Moreover, the content of FF may also reflect the circulating biochemical environment and the changes in blood serum. Despite the known embriyological and histological similarities between right and left ovaries, differences do exist between their venous drainage, anatomical relations and cyclical physiological changes. The difference between the two ovaries in the response to controlled hyperstimulatons (COH) in IVF regimens was later confirmed by recent studies. The aim of this study was to compare the biochemical differences between the FFs yielded from the right and left ovaries in patients undergoing standart IVF treatment.Methods: A prospective observational study was conducted through the unexplained infertility patients with the age between 23-39 years, body mass index (BMI) ≤28 kg/m2, FSH ≤10 mIU/mL. FFs aspirated from the right and the left ovary of the patients were assayed for estradiol, calcium, phosphorus, uric acid, urea, total protein, AST, ALT, glucose, sodium, potassium and creatinine. A total of 10 patients undergoing IVF treatments were included.Results: The investigated parameters were not significantly different between FFs yielded from the right and left ovaries.Conclusion: According to the results of our study, biochemical profile of the aspirated FFs does not significantly differ between the right and the left ovary and consequently, either right or left ovaries provide similar micro-environments to the developing oocytes.

Real options approach for valuation of enterprise resource planning systems

Günümüzde tüm dünya genelindeki sağlık kurumları, hizmet kalitelerini iyileştirmeyi ve maliyetleri düşürmeyi amaçlayan bilişim teknolojileri yatırımlarının önemini kavramışlardır. Sağlık sektörünün sürekli yükselen standartları ve sigorta sistemlerinin artan gereksinimleri karar vericileri, hastanelerde Kurumsal Kaynak Planlama (KKP) sistemlerinin sağlık sektörünün gerek ve ihtiyaçlarına göre uyarlanmış ve geliştirilmiş hali olan Hastane Bilgi Sistemi (HBS) yatırım uygulamalarını dikkate almalarını zorunlu kılmaktadır. Bu tür yatırımların ekonomik değerlemesi karmaşık yapıları nedeniyle her zaman zor olmuştur. Bununla beraber bu yatırımların, bünyelerinde barındırdığı ve firmalara rekabet ortamında avantajlar sağlayan birçok faydası da mevcuttur. Geleneksel iskontolanmış nakit akışı yöntemleri, bu sistemlerin içinde varolan ve yatırım kararını haklı çıkartacak, yönetsel ve stratejik esnekliklerin değerini dikkate almamaktadır. Reel opsiyon yaklaşımı karar vericilere, geleneksel değerleme yöntemlerindeki bu yetersizliklerin üstesinden gelme olanağını sunmaktadır. Bu çalışmada, reel opsiyonları temel alan bir yaklaşımla, birden fazla opsiyonun birarada yer aldığı bir KKP sistemi yatırımının değerlemesine imkan tanıyan bir model önerilmektedir. Binom ağacı modelini temel alarak geliştirilen değerleme modelinin yapısı KKP yatırım fırsatının bünyesindeki birleşik opsiyonları değerlemeye imkan tanıyacak şekilde genişletilmiş ve dolayısıyla karar vericilere yeni bir katma değer sunulmuştur. Geliştirilen modelin potansiyel uygulaması gerçek bir HBS yatırımı değerlemesiyle gösterilmektedir. Yapılan uygulama geleneksel değerleme yöntemlerince kabul edilir görülmeyen yatırımların bile, bünyelerinde barındırdıkları stratejik ve yönetsel unsurlar sayesinde ve gelecekte oluşması muhtemel koşullar dahilinde kabul edilebilir bir hal alabileceğini ortaya koymaktadır. Anahtar Kelimeler: KKP yatırımlarının değerlemesi, reel opsiyonlar, birleşik opsiyonlar, opsiyon değerleme modelleri.Nowadays in order to improve the quality of care delivery and reduce costs, healthcare organizations globally recognize the importance of investing in information technologies (IT). The key drivers of healthcare sector like continuous improvement of healthcare standards and insurance systems have introduced new requirements for hospitals, which in return provided a solid ground for decision-makers to consider implementing hospital information system investment. Hospital information systems (HIS) are the customized and improved versions of enterprise resource planning (ERP) systems, designed according to the needs and the requirements of healthcare sector. It has been always difficult to value such investments in monetary terms due to their complex nature. This type of investments also has many inherent benefits that can provide significant competitive advantages. These inherent benefits can only be viable for decision support if strategic and managerial flexibilities can be utilized. However, traditional capital budgeting techniques, such as net present value (NPV), do not enable decision-makers to consider these flexibilities. Due to its idle valuation approach, NPV only considers a static scenario that ignores the possible dynamic changes in the market affecting future cash flows and interest rates. NPV's passive commitment strategy also forces the decision-makers pre-committing today to a "go" or "no-go" decision considering information only available today. Thus, it is clear that the traditional NPV methods ignore the value of managerial and strategic flexibility inherent in the investments which undermines a company's strategic opportunities. Investment science experts agreed that it is a must to integrate financial and strategic issues and study them together for making an investment decision. Recognizing the shortcomings of traditional NPV methods, real options (RO) technique has been suggested as an alternative approach. RO whose methodology is based upon financial options theory enable the decisions makers to analyze the true value of a real life investment opportunity through better modeling of the uncertainty within the investment. RO method does not only value the managerial flexibility through the investment horizon, it also supports and expands the firm's strategic frame. It gives a road map to the managers to optimize the medium and long-term strategies according to the expectations. RO can be categorized to six main managerial options according to the flexibility they have: option to defer, option to stage, option to expand/contract, option to abandon, option to switch and growth option. In a real-life investment scenario it is usually viable to have more than one real option concurrently. The first applications of real options are about mining and operating natural resource investments. Besides, the new application areas for RO are growing rapidly. Regardless of an industry, it can be used in many cases like entering a new market, deciding the initial scale of an investment, scaling up or down in the future according to the needs, managing multi-staged complex projects, and freezing up or completely ending an investment. Developing with dynamic market conditions due to the continuous improvements, IT investments are also an appropriate application area for RO. This study introduces a RO based methodology that enables decision-makers to value an ERP system investment incorporating multiple options. The option valuation model developed in this study extends the binomial lattice framework to model an ERP investment opportunity with compound options. The potential application of the proposed model is presented to evaluate an HIS implementation project of a Turkish health care organization. For this purpose, considering the ever changing dynamics of the market affecting possible cash flows, a computer-based decision support framework is developed to provide a flexible and efficient solution to decision-makers. In this manner, for simulating various possible future market conditions, different scenarios are generated via triggering conditional changes in model variables and parameters. Results are interpreted with tables and graphs for submitting a thorough road map to decision-makers that can be utilized as a managerial asset for understanding the interactions between model parameters. Further investigations have shown that even an investment that is not approved to be undertaken with traditional valuation methods, can be valuable for a firm considering the strategic and managerial flexibilities inherent in the investment, with the effect of the possible market conditions in the future. Thus, it is also emphasized that real options approach generally presents a new point of view for valuing IT investment projects. Keywords: Evaluation of ERP investments, real options, compound options, option valuation models

Microsatellite diversity of the Nordic type of goats in relation to breed conservation: how relevant is pure ancestry?

In the last decades, several endangered breeds of livestock species have been re-established effectively. However, the successful revival of the Dutch and Danish Landrace goats involved crossing with exotic breeds and the ancestry of the current populations is therefore not clear. We have generated genotypes for 27 FAO-recommended microsatellites of these landraces and three phenotypically similar Nordic-type landraces and compared these breeds with central European, Mediterranean and south-west Asian goats. We found decreasing levels of genetic diversity with increasing distance from the south-west Asian domestication site with a south-east-to-north-west cline that is clearly steeper than the Mediterranean east-to-west cline. In terms of genetic diversity, the Dutch Landrace comes next to the isolated Icelandic breed, which has an extremely low diversity. The Norwegian coastal goat and the Finnish and Icelandic landraces are clearly related. It appears that by a combination of mixed origin and a population bottleneck, the Dutch and Danish Land-races are separated from the other breeds. However, the current Dutch and Danish populations with the multicoloured and long-horned appearance effectively substitute for the original breed, illustrating that for conservation of cultural heritage, the phenotype of a breed is more relevant than pure ancestry and the genetic diversity of the original breed. More in general, we propose that for conservation, the retention of genetic diversity of an original breed and of the visual phenotype by which the breed is recognized and defined needs to be considered separately

Detection of bovine leukocyte adhesion deficiency (BLAD) in Turkish native and Holstein cattle

The purpose of this work was to study whether the bovine leukocyte adhesion deficiency ( BLAD) allele is present in native cattle breeds and the Holstein breed in Turkey. Blood samples were obtained from 120 Holstein, 20 Brown Swiss, 20 Anatolian Black, 20 Turkish Grey, 20 South Anatolian Red and 20 East Anatolian Red cattle. The isolated DNA materials were multiplied in PCR using the primer developed by Kriegesmann et al. ( 1997). In order to determine the area of mutation in PCR products, the PCR products were digested with TaqI endonuclease enzyme. The resulting fragments were analysed on 2% agarose gel for the absence of a TaqI restriction site. It was found that two of the Holstein cattle ( a bull and a cow) were heterozygote BLAD carriers. There was no homozygote BLAD animal. The BLAD allele was not found in the other breeds used in the study. The mutant BLAD allele frequency in the 120 Holstein cattle calculations was 0.0084