Aquaponics: alternative types and approaches

Whilst aquaponics may be considered in the mid-stage of development, there are a number of allied, novel methods of food production that are aligning alongside aquaponics and also which can be merged with aquaponics to deliver food efficiently and productively. These technologies include algaeponics, aeroponics, aeroaquaponics, maraponics, haloponics, biofloc technology and vertical aquaponics. Although some of these systems have undergone many years of trials and research, in most cases, much more scientific research is required to understand intrinsic processes within the systems, efficiency, design aspects, etc., apart from the capacity, capabilities and benefits of conjoining these systems with aquaponics

Oleanolic acid controls allergic and inflammatory responses in experimental allergic conjunctivitis

This is an open-access article distributed under the terms of the Creative Commons Attribution License.Pollen is the most common aeroallergen to cause seasonal conjunctivitis. The result of allergen exposure is a strong Th2-mediated response along with conjunctival mast cell degranulation and eosinophilic infiltration. Oleanolic acid (OA) is natural a triterpene that displays strong anti-inflammatory and immunomodulatory properties being an active anti-allergic molecule on hypersensitivity reaction models. However, its effect on inflammatory ocular disorders including conjunctivits, has not yet been addressed. Hence, using a Ragweed pollen (RWP)-specific allergic conjunctivitis (EAC) mouse model we study here whether OA could modify responses associated to allergic processes. We found that OA treatment restricted mast cell degranulation and infiltration of eosinophils in conjunctival tissue and decreased allergen-specific Igs levels in EAC mice. Th2-type cytokines, secreted phospholipase A2 type-IIA (sPLA2-IIA), and chemokines levels were also significantly diminished in the conjunctiva and serum of OA-treated EAC mice. Moreover, OA treatment also suppressed RWP-specific T-cell proliferation. In vitro studies, on relevant cells of the allergic process, revealed that OA reduced the proliferative and migratory response, as well as the synthesis of proinflammatory mediators on EoL-1 eosinophils and RBL-2H3 mast cells exposed to allergic and/or crucial inflammatory stimuli such as RWP, sPLA2-IIA or eotaxin. Taken together, these findings demonstrate the beneficial activity of OA in ocular allergic processes and may provide a new intervention strategy and potential therapy for allergic diseases. © 2014 Córdova et al.The study was supported by grants from the Spanish MINECO (reference SAF2009-08407). Patricia Gallego-Muñoz and Claudia Cordova were funded by the FPI Program from the Government of Castilla y León (co-funded by FSE). Rubén Martín was supported by the Sara Borrell Program from the Spanish MINECO.Peer Reviewe

New evidence on the role of remittances on healthcare expenditures by Mexican households

Remittances, Household expenditures, Healthcare, Mexico, F24, I1,

Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicing

The spliceosome is a dynamic ribonucleoprotein (RNP) machine that catalyzes the removal of introns in the two transesterification steps of eukaryotic pre-mRNA splicing. Here we used single molecule fluorescence resonance energy transfer to monitor the distance of the 5′ splice site (5′SS) and branchpoint (BP) of pre-mRNA in affinity-purified spliceosomes stalled by a mutation in the DExD/H-box helicase Prp2 immediately prior to the first splicing step. Addition of recombinant Prp2 together with NTP and protein cofactor Spp2 rearranges the spliceosome-substrate complex to reversibly explore conformations with proximal 5′SS and BP that accommodate chemistry. Addition of Cwc25 then strongly biases this equilibrium towards the proximal conformation, promoting efficient first-step splicing. The spliceosome thus functions as a biased Brownian ratchet machine where a helicase unlocks thermal fluctuations subsequently rectified by a cofactor “pawl”, a principle possibly widespread among the many helicase-driven RNPs