Post-harvesting conservation of prosopis juliflora's pod in Alagoas

This study had the aim to evaluate the influence of different dehydration and storage periods and fungi occurrence on the carob pod chemical composition. The work was conducted in the Universidade Federal de Alagoas-Brazil, during September to December, 2002. The experimental design was entirely randomized with a 3 x 3 outline. The nine treatments, resulted from combination of the factors dehydration (24 hours under shadow exposure; 12 and 24 hours under solar exposition) and storage (pods non-storage; pods storage by 30 and 60 days), were composed by 4 replications. Before analyzing process, the material was dried in regulated stove to 65°C with forced circulation, crushed and conserved in regulated freezer to -5°C. The studied variables were: dry matter (DM), crude protein (CP), ether extract (EE), crude fiber (CF) and mineral matter (MM). The results indicate that the dehydration influenced significantly (p<0.05) the MM. In relation to storage, there was significant difference (p<0.05) for all analyzed variables. It was observed significant interaction (p<0.05) for CP, CF and MM

Post-harvesting conservation of prosopis juliflora's pod in Alagoas

This study had the aim to evaluate the influence of different dehydration and storage periods and fungi occurrence on the carob pod chemical composition. The work was conducted in the Universidade Federal de Alagoas-Brazil, during September to December, 2002. The experimental design was entirely randomized with a 3 x 3 outline. The nine treatments, resulted from combination of the factors dehydration (24 hours under shadow exposure; 12 and 24 hours under solar exposition) and storage (pods non-storage; pods storage by 30 and 60 days), were composed by 4 replications. Before analyzing process, the material was dried in regulated stove to 65°C with forced circulation, crushed and conserved in regulated freezer to -5°C. The studied variables were: dry matter (DM), crude protein (CP), ether extract (EE), crude fiber (CF) and mineral matter (MM). The results indicate that the dehydration influenced significantly (p<0.05) the MM. In relation to storage, there was significant difference (p<0.05) for all analyzed variables. It was observed significant interaction (p<0.05) for CP, CF and MM

Diversidade genética de Begomovirus que infectam plantas invasoras na região nordeste Genetic diversity of Begomovirus infecting weeds in northeastern Brazil

Os Begomovirus fazem parte de uma família numerosa de fitovírus denominada Geminiviridae. Eles infectam ampla gama de hospedeiras, incluindo muitas espécies cultivadas, como tomate (Lycopersicon esculentum), feijão (Phaseolus vulgaris), pimentão (Capsicum annuum), caupi (Vigna unguiculata), mandioca (Manihot esculenta) etc., além de plantas invasoras de várias espécies. Em alguns casos, plantas invasoras podem funcionar como reservatórios desses vírus para plantas cultivadas, mediante transmissão pelo inseto-vetor. No presente trabalho, plantas invasoras com sintomas de mosaico amarelo, deformação do limbo foliar e redução do crescimento foram avaliadas no tocante à presença de Begomovirus mediante a técnica de PCR, empregando-se oligonucleotídeos universais para detecção desses vírus. Foram avaliadas 11 amostras, correspondendo a 10 espécies, coletadas em municípios dos Estados de Alagoas, Pernambuco e Bahia. Algumas, como Herissantia crispa, Waltheria indica e Triumfetta semitriloba, são relatadas pela primeira vez como espécies hospedeiras de Begomovirus. Para estimar a variabilidade genética dos Begomovirus detectados, o produto de amplificação dos diversos isolados foi clivado com as enzimas de restrição EcoRI, HinfI e TaqI. Confirmando resultados obtidos para plantas cultivadas por outros grupos de pesquisa, foram observados padrões distintos de clivagem para os isolados estudados, evidenciando a grande variabilidade genética desses vírus.<br>Genus Begomovirus belong to the family Geminiviridae. Begomovirus is associated with a wide range of hosts, including many cultivated species such as tomato (Lycopersicon esculentum), dry beans (Phaseolus vulgaris), pepper (Capsicum annuum), cowpea (Vigna unguiculata), cassava (Manihot esculenta), etc., besides many weed species. It has been demonstrated that in some cases weeds act as virus reservoirs for cultivated plants. In the present work, weed samples presenting yellow mosaic, foliar malformation and size reduction were tested by PCR for infection by Begomovirus, using specific degenerate oligonucleotides. Eleven samples corresponding to 10 plant species were collected in the countryside towns in the states of Alagoas, Pernambuco and Bahia. Some plant species such as Herissantia crispa, Waltheria indica and Triumfetta semitriloba are reported for the first time as hosts for Begomovirus. To estimate the genetic diversity of the detected Begomovirus, the amplified products of several isolates were cleaved with each three restriction enzymes, EcoRI, HinfI, and TaqI. Different patterns were observed for the studied isolates, pointing out to a great genetic diversity for these viruses