Pseudomonas aeruginosa induces p38MAP kinase-dependent IL-6 and CXCL8 release from bronchial epithelial cells via a Syk kinase pathway

Pseudomonas aeruginosa (Pa) infection is a major cause of airway inflammation in immunocompromised and cystic fibrosis (CF) patients. Mitogen-activated protein (MAP) and tyrosine kinases are integral to inflammatory responses and are therefore potential targets for novel anti-inflammatory therapies. We have determined the involvement of specific kinases in Pa-induced inflammation. The effects of kinase inhibitors against p38MAPK, MEK 1/2, JNK 1/2, Syk or c-Src, a combination of a p38MAPK with Syk inhibitor, or a novel narrow spectrum kinase inhibitor (NSKI), were evaluated against the release of the proinflammatory cytokine/chemokine, IL-6 and CXCL8 from BEAS-2B and CFBE41o- epithelial cells by Pa. Effects of a Syk inhibitor against phosphorylation of the MAPKs were also evaluated. IL-6 and CXCL8 release by Pa were significantly inhibited by p38MAPK and Syk inhibitors (p<0.05). Phosphorylation of HSP27, but not ERK or JNK, was significantly inhibited by Syk kinase inhibition. A combination of p38MAPK and Syk inhibitors showed synergy against IL-6 and CXCL8 induction and an NSKI completely inhibited IL-6 and CXCL8 at low concentrations. Pa-induced inflammation is dependent on p38MAPK primarily, and Syk partially, which is upstream of p38MAPK. The NSKI suggests that inhibiting specific combinations of kinases is a potent potential therapy for Pa-induced inflammation

Efficient routing of single photons by one atom and a microtoroidal cavity

Single photons from a coherent input are efficiently redirected to a separate output by way of a fiber-coupled microtoroidal cavity interacting with individual Cesium atoms. By operating in an overcoupled regime for the input-output to a tapered fiber, our system functions as a quantum router with high efficiency for photon sorting. Single photons are reflected and excess photons transmitted, as confirmed by observations of photon antibunching (bunching) for the reflected (transmitted) light. Our photon router is robust against large variations of atomic position and input power, with the observed photon antibunching persisting for intracavity photon number 0.03 \lesssim n \lesssim 0.7

The Origin of the Dust Arch in the Halo of NGC 4631: An Expanding Superbubble?

We study the nature and the origin of the dust arch in the halo of the edge-on galaxy NGC 4631 detected by Neininger & Dumke (1999). We present CO observations made using the new On-The-Fly mapping mode with the FCRAO 14m telescope, and find no evidence for CO emission associated with the dust arch. Our examination of previously published HI data shows that if previous assumptions about the dust temperature and gas/dust ratio are correct, then there must be molecular gas associated with the arch, below our detection threshold. If this is true, then the molecular mass associated with the dust arch is between 1.5 x 10^8 M(sun)and 9.7 x 10^8 M(sun), and likely towards the low end of the range. A consequence of this is that the maximum allowed value for the CO-to-H_2 conversion factor is 6.5 times the Galactic value, but most likely closer to the Galactic value. The kinematics of the HI apparently associated with the dust arch reveal that the gas here is not part of an expanding shell or outflow, but is instead two separate features (a tidal arm and a plume of HI sticking out into the halo) which are seen projected together and appear as a shell. Thus there is no connection between the dust "arch" and the hot X-ray emitting gas that appears to surround the galaxy Wang et al. (2001).Comment: 14 pages, including 4 figures. Accepted by A.J. for March 200

Economic Impact of Withdrawing Specific Agricultural Pesticides in the Lower Rio Grande Valley

The Air, Pesticides, and Toxics Division of the Environmental Protection Agency (EPA) has encouraged all states to develop a plan to manage the use of pesticides to prevent application that would result in unreasonable risks to human health and the environment from contamination of ground water. In February, 1988, EPA proposed a strategy where by they would regulate certain pesticides by prohibiting their use in areas vulnerable to leaching unless a state develops and implements a management plan acceptable to EPA. However, banning the use of a pesticide in a region is the worst case scenario available to the TWC for managing water quality. The Texas Water Commission (TWC) assessed the State for areas vulnerable to leaching and found the Lower Rio Grande Valley (LRGV) to be a highly vulnerable area. This study examines three pesticides (atrazine, dicrotophos, and aldicarb) currently used in the LRGV that were identified by the TWC as potential contaminants of ground water. Alternative methods of controlling pests in this region were identified, and the economic impacts of withdrawing one or all three of these pesticides from the study area were estimated. Regional impacts on gross receipts (sales), variable costs, and net returns were determined. If atrazine use were banned in the LRGV, corn and sorghum sales would decrease by approximately $1 million, variable costs to produce corn, sorghum, and sugarcane would increase by almost$2 million dollars, leaving farmers in the region with a $3 million dollar 1088 in net income per year. If dicrotophos use were prohibited in the LRGV, variable cost to produce cotton would increase by over$600,000 for the region as a whole. Banning aldicarb use in the study area would reduce citrus sales by almost $3 million, increase variable costs to produce citrus by over$200,000, and reduce farmer net income by over $3 million annually

Winter barley : a new factor in Missouri agriculture

Cover title.Includes bibliographical references

An all-year pasture system for Missouri

Caption title.Digitized 2006 AES MoU

An all-year pasture system for Missouri

Caption title.Digitized 2006 AES MoU