Купцы и дворяне Лугинины: три поколения владельцев Златоустовских заводов второй половины XVIII в.

The article presents the history of the ownership of Zlatoust mining plants by three generations of the Luginin family. It shows direct dependence of the dynamics of ownership and inheritance of plants on the vicissitudes of the family history. It is established that the founder of the Zlatoust factory farm, L. I. Luginin, relied in his business activities on the help of his son Maxim, who took over the management and control of a new part of the family business — the Ural factories in 1769. M. L. Luginin died in the middle of 1770s and L. I. Luginin was left without an assistant and a trained heir. The grandchildren of the factory owner, due to the life circumstances associated with their new noble status, character traits and age, failed to adopt the grandfather’s experience and did not become an equivalent replacement for their father. After the death of the founder, during the period of guardianship management of the factories, the middle grandson, Larion Maksimovich, showed interest in the factories, but he also died before he had been in the factories for two years. The transfer of ownership of the district to the most unworthy of the heirs, I. M. Luginin, eventually led to the exit of the estate from the possession of the family. Zlatoust factories, located far from the rest of the family’s possessions, required constant control of the owner, his participation in the management. In this case, the succession should have been manifested in the active participation of the heirs in the management of the district, personal control over the execution of orders. It is concluded that the succession of generations of the family is of great importance in the early stages of the organization of the business, when the personal control of the owner is a significant factor in the preservation of the business in the hands of the family. © 2021 Institute of History and Archeology of the Ural Branch of RAS. All rights reserved

Stability of the film flow passing round the cross-section elements in the area of high density irrigation

The paper studies the loss of stability of a liquid film using regular surface roughness. The model of the breakdown process is described and the conditions for the formation of a liquid film breakdown are determined experimentally. It was found that there are two characteristic modes, namely drop and film regimes. Using the methods of thermal anemometry, data were obtained on the distribution of the droplet sizes after a film breakdown and the dependence of the We number on the projection arrangement height was plotted. © Published under licence by IOP Publishing Ltd.This work was supported by the Russian Foundation for Basic Research, grant No. 17-38-50069

Chlamydia pan-genomic analysis reveals balance between host adaptation and selective pressure to genome reduction

Background Chlamydia are ancient intracellular pathogens with reduced, though strikingly conserved genome. Despite their parasitic lifestyle and isolated intracellular environment, these bacteria managed to avoid accumulation of deleterious mutations leading to subsequent genome degradation characteristic for many parasitic bacteria. Results We report pan-genomic analysis of sixteen species from genus Chlamydia including identification and functional annotation of orthologous genes, and characterization of gene gains, losses, and rearrangements. We demonstrate the overall genome stability of these bacteria as indicated by a large fraction of common genes with conserved genomic locations. On the other hand, extreme evolvability is confined to several paralogous gene families such as polymorphic membrane proteins and phospholipase D, and likely is caused by the pressure from the host immune system. Conclusions This combination of a large, conserved core genome and a small, evolvable periphery likely reflect the balance between the selective pressure towards genome reduction and the need to adapt to escape from the host immunity

Extraction of MO from Model Solutions for Processing Nickel-Aluminum-Cobalt Catalysts

A method of extraction extraction of molybdenum from AMC catalysts has been developed. The kinetics of the extraction process was studied. As well as the effect of soda concentration and temperature dependence on the degree of molybdenum extraction

REDD1 Protects Osteoblast Cells from Gamma Radiation-Induced Premature Senescence

Radiotherapy is commonly used for cancer treatment. However, it often results in side effects due to radiation damage in normal tissue, such as bone marrow (BM) failure. Adult hematopoietic stem and progenitor cells (HSPC) reside in BM next to the endosteal bone surface, which is lined primarily by hematopoietic niche osteoblastic cells. Osteoblasts are relatively more radiation-resistant than HSPCs, but the mechanisms are not well understood. In the present study, we demonstrated that the stress response gene REDD1 (regulated in development and DNA damage responses 1) was highly expressed in human osteoblast cell line (hFOB) cells after γ irradiation. Knockdown of REDD1 with siRNA resulted in a decrease in hFOB cell numbers, whereas transfection of PCMV6-AC-GFP-REDD1 plasmid DNA into hFOB cells inhibited mammalian target of rapamycin (mTOR) and p21 expression and protected these cells from radiation-induced premature senescence (PS). The PS in irradiated hFOB cells were characterized by significant inhibition of clonogenicity, activation of senescence biomarker SA-β-gal, and the senescence-associated cytokine secretory phenotype (SASP) after 4 or 8 Gy irradiation. Immunoprecipitation assays demonstrated that the stress response proteins p53 and nuclear factor κ B (NFkB) interacted with REDD1 in hFOB cells. Knockdown of NFkB or p53 gene dramatically suppressed REDD1 protein expression in these cells, indicating that REDD1 was regulated by both factors. Our data demonstrated that REDD1 is a protective factor in radiation-induced osteoblast cell premature senescence

p53 Amino-Terminus Region (1–125) Stabilizes and Restores Heat Denatured p53 Wild Phenotype

BACKGROUND:The intrinsically disordered N-ter domain (NTD) of p53 encompasses approximately hundred amino acids that contain a transactivation domain (1-73) and a proline-rich domain (64-92) and is responsible for transactivation function and apoptosis. It also possesses an auto-inhibitory function as its removal results in remarkable reduction in dissociation of p53 from DNA. PRINCIPAL FINDINGS/METHODOLOGY:In this report, we have discovered that p53-NTD spanning amino acid residues 1-125 (NTD125) interacted with WT p53 and stabilized its wild type conformation under physiological and elevated temperatures, both in vitro and in cellular systems. NTD125 prevented irreversible thermal aggregation of heat denatured p53, enhanced p21-5'-DBS binding and further restored DBS binding activity of heat-denatured p53, in vitro, in a dose-dependent manner. In vivo ELISA and immunoprecipitation analysis of NTD125-transfected cells revealed that NTD125 shifted equilibrium from p53 mutant to wild type under heat stress conditions. Further, NTD125 initiated nuclear translocation of cytoplasmic p53 in transcriptionally active state in order to activate p53 downstream genes such as p21, Bax, PUMA, Noxa and SUMO. CONCLUSION/SIGNIFICANCE:Here, we showed that a novel chaperone-like activity resides in p53-N-ter region. This study might have significance in understanding the role of p53-NTD in p53 stabilization, conformational activation and apoptosis under heat-stress conditions

SORPTION RECOVERY OF RHENIUM FROM SULFURIC ACID SOLUTIONS

The possibility of rhenium extraction from simulated solutions using weak base anion exchange resins was researched. It was found that rhenium is adsorbed by these resins completely. The most selective resin was chosen.Работа выполнена при финансовой поддержке Минобрнауки России, соглашение о предоставлении субсидии от 26.09.2017 г. № 14.575.21.0137 (уникальный идентификатор соглашения RFMEFI57517X0137), в рамках ФЦП “Исследования и разработки по приоритетным направлениям развития научно-технологического комплекса России на 2014–2020 годы”

Случай тяжелого трихинеллеза у девочки-подростка в Самарской области

Discribe the case of severe flow of а trichinosis in a girl 15 years old. The symptoms are an acute to x ico-allergological syndrome, generalized swelling myalgia and myasthenia with the full adynamia, infectios-toxicology kidney, an acute hypoalbuminemia with development polyserositis, adsence eosinophilia.Описан случай тяжелого течения трихинеллеза у девочки 15 лет, маркерами которого являлись: выраженный токсико-аллергический синдром, генерализованные отеки, миалгии и миастении с полной адинамией, инфекционно-токсическая почка, выраженная гипоальбуминемия с развитием полисерозита, отсутствие эозинофилии