Porphyrins profile by high performance liquid chromatography/electrospray ionization tandem mass spectrometry for the diagnosis of porphyria

Porphyrias are a group of inherited or acquired disorders of certain enzymes in the heme bio-synthetic pathway. Most porphyria symptoms are nonspecific and occur intermittently; resulting frequently in missed diagnosis since the disease itself is a rare one. The aim of the study is toestablish a new reliable and accurate laboratory method for separation, identification and quantitation of urinary porphyrins by liquid chromatography tandem mass spectrometry (LC/MS/MS) and thereby the diagnosis of different porphyria types for the first time in Egypt. Screening by plasma fluorescence and quantitative determination of urinary porphyrins by high performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC/ESI/MS/MS) of 50 clinically suspected patients revealed one case of variegate porphyria and five cases of porphyria cutanea tarda. Plasma fluorescence scanning is a simple procedure that can be used as screening test to detect porphyria patients that require quantitation of urinary porphyrins as a second step. Quantitative determination of urinary porphyrins using HPLC/ESI/MS/MS and ion mapping techniques are applicable for the differential diagnosis of porphyria types, since each type has a characteristic porphyrins excretion profile. Quantitative determination of urinary porphyrins by HPLC/ESI/MS/MS used in this study is a modification for the method Stoev et al. while ion mapping technique is a new technique invented by the research team at the Biochemical Genetics Department

Maintained partial protection against Streptococcus pneumoniae despite B‐cell depletion in mice vaccinated with a pneumococcal glycoconjugate vaccine

Objectives: Anti-CD20 monoclonal antibody therapy rapidly depletes > 95% of CD20+ B cells from the circulation. B-cell depletion is an effective treatment for autoimmune disease and B-cell malignancies but also increases the risk of respiratory tract infections. This effect on adaptive immunity could be countered by vaccination. We have used mouse models to investigate the effects of B-cell depletion on pneumococcal vaccination, including protection against infection and timing of vaccination in relation to B-cell depletion. // Methods: C57BL/6 female mice were B-cell depleted using anti-CD20 antibody and immunized with two doses of Prevnar-13 vaccine either before or after anti-CD20 treatment. B-cell repertoire and Streptococcus pneumoniae–specific IgG levels were measured using whole-cell ELISA and flow cytometry antibody-binding assay. Protection induced by vaccination was assessed by challenging the mice using a S. pneumoniae pneumonia model. // Results: Antibody responses to S. pneumoniae were largely preserved in mice B-cell depleted after vaccination resulting in full protection against pneumococcal infections. In contrast, mice vaccinated with Prevnar-13 while B cells were depleted (with > 90% reduction in B-cell numbers) had decreased circulating anti–S. pneumoniae IgG and IgM levels (measured using ELISA and flow cytometry antibody binding assays). However, some antibody responses were maintained, and, although vaccine-induced protection against S. pneumoniae infection was impaired, septicaemia was still prevented in 50% of challenged mice. // Conclusions: This study showed that although vaccine efficacy during periods of profound B-cell depletion was impaired some protective efficacy was preserved, suggesting that vaccination remains beneficial

Interleukin 6 (IL6) as a predictor outcome in patients with compensated cirrhosis and symptomatic gall stones after cholecystectomy

Compensated cirrhosis means that the liver is heavily scarred but can still perform many important functions; many peoples with compensated cirrhosis have gall bladder stones. The advantages of laparoscopic cholecystectomy (LC) for most patients have been extensively published. However its benefits and successful use in patients with cirrhosis are less documented. The study compromised 50 patients with symptomatic gallstone in compensated liver disease and undergone either open cholecystectomy (OC) or laparoscopic cholecystectomy. These patients were randomized into two groups: Group I included 24 patients who underwent OC, and group II included 26 patients who underwent LC. Patient’s age, sex, clinical presentation and Child-Turcotte-Pugh (CTP) class were documented. No patients in this study had CTP class c cirrhosis. IL-6 was measured by ELISA, postoperative pain (measured by Visual analog scale), hospital stay, blood loss, recovery time (return to work), and liver function tests were documented. IL-6 was significantly lowered at 6th hour and 12th hour post operative in LC group. Mean surgical time was significantly longer in OC than LC group, (mean ±SD, 96.6 ±32 vs 58.7 ± 23.8 min, P = 0.037). No patients in group II required any blood replacement in contrast to 9 patients (37.5%) in group I. Intraoperative bleeding remained significantly higher in group I (P = 0.043). No patients in group II had wound complications compared with 5 patients (29.14%) in group I. Group I had significantly longer hospital stay than group II, mean 9.0+ 1.3 days (median 7) vs 2.3 days + 1.9 (median 2.5); P = 0.001. Our results were demonstrated that laparoscopic cholecystectomy can be performed safely in patients with CTP class A and B cirrhosis. IL-6 was more significantly, increased post operatively in open cholecystectomy than laparoscopic one and it correlated well with intensity of operative trauma. Keywords: Interleukin 6; Liver cirrhosis; Cholecystectom

An Investigation into Mechanical Properties of Ductile Cast Iron with Different Heat Treatment Processes

The mechanical properties as well as microstructure of the ductile cast iron (DCI) are most likely affected by heat treatments. In this work, the mechanical properties of different heat treated of DCI alloy were investigated. Two heat treatment (HT) processes were conducted for DCI; austempering and quenching followed by lower tempering. The melted alloy of DCI was poured in Y-block, from which the specimens of the mechanical tests were prepared. Experimental tests were carried out to investigate the effect of these HT processes on the mechanical properties. A comparison between mechanical properties due to HT and as cast DCI are presented and discussed. The results showed that there is a difference in microstructure, homogeneity, wear rate and compression of DCI based on the conducted heat treatment

Simplicity versus complexity in modelling groundwater recharge in Chalk catchments

Models of varying complexity are available to provide estimates of recharge in headwater Chalk catchments. Some measure of how estimates vary between different models can help guide the choice of model for a particular application. This paper compares recharge estimates derived from four models employing input data at varying spatial resolutions for a Chalk headwater catchment (River Pang, UK) over a four-year period (1992-1995) that includes a range of climatic conditions. One model was validated against river flow data to provide a measure of their relative performance. Each model gave similar total recharge for the crucial winter recharge period when evaporation is low. However, the simple models produced relatively lower estimates of the summer and early autumn recharge due to the way in which processes governing recharge especially evaporation and infiltration are represented. The relative uniformity of land use, soil types and rainfall across headwater, drift-free Chalk catchments suggests that complex, distributed models offer limited benefits for recharge estimates at the catchment scale compared to simple models. Nonetheless, distributed models would be justified for studies where the pattern and amount of recharge need to be known in greater detail and to provide more reliable estimates of recharge during years with low rainfall.</p> <p style='line-height: 20px;'><b>Keywords:</b> Chalk, modelling, groundwater recharge</p

Potential Role of New Anthropometric Parameters in Childhood Obesity with or Without Metabolic Syndrome

BACKGROUND: Obese children and adolescents are more prone to have metabolic syndrome (MS).MS is a cluster of cardiovascular risk factors associated with insulin resistance. Body round index [BRI], visceral adiposity index [VAI] and a body shape index [ABSI] are among the new obesity anthropometric parameters. AIM: To evaluate the new markers for obesity in children and their possible association with other laboratory and clinical variables of MS. METHODS: Eighty nine obese children and 40 controls aged 10-18 years were recruited. Full history taking, thorough clinical examination, anthropometric and biochemical features were performed in the studied groups. Subcutaneous fat thickness (SFT) and visceral fat thickness (VFT) were estimated by ultrasonography. RESULTS: Obese children, exhibited significantly higher values in all anthropometric measurements (P &lt; 0.001). Diastolic and systolic blood pressure were significantly higher (P &lt; 0.001) in the obese group. ABSI, BRI and VAI have been found to be significantly higher in obese subjects (P &lt; 0.001), with no significant gender difference. BMI, WHtR, WC/HR, SBP, DBP, subcutaneous fat thickness and visceral fat thickness, Liver Span, ABSI, BRI, VAI and HOMA_IR were significantly higher among children with MS than those without MS. Positive significant correlations of VAI with BMI, WC/Ht, WC/Hip, SBP, DBP, SFT, VFT, Liver size and HOMA-IR (r = 0.384, 0.239, 0.268, 0.329, 0.516, 0.320, 0.254, 0.251, and 0.278 respectively) are shown. The area under the ROC curve (AUC) of BMI, VAI, ABSI, BRI for predicting MS was 0.802 (0.701-0.902), 0.737 (0.33-0.841), 0.737 (0.620-0.855), 0.816 (0.698-0.934). CONCLUSION: We suggest using the VAI and WHtR indexes, as they are better predictor of MS

Effect of different levels of NPK on wheat crop in North Delta

A balanced fertilization of NPK nutrients leads to increase soil fertility, anti soil nutrients depletion and also decrease nutrients losses to ground water and hence decrease its pollution. Therefore, a field experiment was conducted for two growing seasons on wheat in North Delta, Egypt. Different levels of nitrogen (0, 96, 192 and 288 kg N ha-1), phosphorus (0 and 53 kg P2O5 ha-1) and potassium (0 and 120 kg K2O ha-1) as well as 4 doses of N fertilizer were used. The objective of the present study was to investigate the effect of previous different levels of N, P and K fertilization and N fertilizer splitting on wheat yield and its components as well as uptake of N, P and K by wheat grain under different treatments. The results revealed that yield and its components were significantly affected by graded applied and splitting of N levels. The protein content in wheat grains was increased with increasing N levels up to 288 kg N ha-1 in presence of 53 kg P2O5 ha-1 as well as 120 kg K2O ha-1. The previous treatment recorded the highest values for protein content in wheat grains and total chlorophyll content