User's manual for the Shuttle Electric Power System analysis computer program (SEPS), volume 2 of program documentation

The Shuttle Electric Power System Analysis SEPS computer program which performs detailed load analysis including predicting energy demands and consumables requirements of the shuttle electric power system along with parameteric and special case studies on the shuttle electric power system is described. The functional flow diagram of the SEPS program is presented along with data base requirements and formats, procedure and activity definitions, and mission timeline input formats. Distribution circuit input and fixed data requirements are included. Run procedures and deck setups are described

Noise Properties of Two Mutually Coupled Spin-Transfer Nanooscillators in the Phase Locking Regime

Introduction. Today, many research endeavors are devoted to the miniaturization of microwave sources. One of the promising approaches is the use of magnetic nanostructures (spintronics elements), providing a wide range of frequency tuning and low power consumption. The main disadvantage of spintronics generators (spintransfer nanoscillators ‒ STNO) is a low output power of generated oscillations (tens of nanowatts and less). A possible solution is to sum up the power of many STNOs in a mutual synchronization mode.Aim. The investigation of noise properties of two connected STNOs with identical and non-identical parameters in a phase synchronization mode.Materials and methods. A model was developed of two STNOs interconnected by spin waves taking into account thermal noises. Spectral power densities of the amplitude and phase noise were obtained by the method of effective linearization.Results. Dependencies were obtained in a general form for attenuation coefficients of the amplitude and phase fluctuations of noise sources for each STNO. Three cases of synchronization were considered: completely identical STNOs, two identical STNOs but with different oscillation frequencies, and two non-identical STNOs, differing in an allowance of self-excitation by frequencies and amplitudes of the oscillations. It was possible to obtain a gain in the amplitude and phase noise for two identical STNOs. In this case, an increase in the allowance of self-excitation led to a decrease in the level of phase and amplitude noise.Conclusion. This analysis of the attenuation coefficients for non-identical STNOs demonstrates the possibility of improving the noise properties of each of the generators. In this case, the best noise value is obtained for an STNO with greater stability in a stand-alone mode.Introduction. Today, many research endeavors are devoted to the miniaturization of microwave sources. One of the promising approaches is the use of magnetic nanostructures (spintronics elements), providing a wide range of frequency tuning and low power consumption. The main disadvantage of spintronics generators (spintransfer nanoscillators ‒ STNO) is a low output power of generated oscillations (tens of nanowatts and less). A possible solution is to sum up the power of many STNOs in a mutual synchronization mode.Aim. The investigation of noise properties of two connected STNOs with identical and non-identical parameters in a phase synchronization mode.Materials and methods. A model was developed of two STNOs interconnected by spin waves taking into account thermal noises. Spectral power densities of the amplitude and phase noise were obtained by the method of effective linearization.Results. Dependencies were obtained in a general form for attenuation coefficients of the amplitude and phase fluctuations of noise sources for each STNO. Three cases of synchronization were considered: completely identical STNOs, two identical STNOs but with different oscillation frequencies, and two non-identical STNOs, differing in an allowance of self-excitation by frequencies and amplitudes of the oscillations. It was possible to obtain a gain in the amplitude and phase noise for two identical STNOs. In this case, an increase in the allowance of self-excitation led to a decrease in the level of phase and amplitude noise.Conclusion. This analysis of the attenuation coefficients for non-identical STNOs demonstrates the possibility of improving the noise properties of each of the generators. In this case, the best noise value is obtained for an STNO with greater stability in a stand-alone mode

Program manual for the Shuttle Electric Power System analysis computer program (SEPS), volume 1 of program documentation

The Shuttle Electric Power System (SEPS) computer program is considered in terms of the program manual, programmer guide, and program utilization. The main objective is to provide the information necessary to interpret and use the routines comprising the SEPS program. Subroutine descriptions including the name, purpose, method, variable definitions, and logic flow are presented

Detection of natural killer T cells in mice infected with Rickettsia conorii

Little information is available regarding the role of natural killer T (NKT) cells during the early stage of Rickettsia conorii infection. Herein, C3H/HeN mice were infected with the Malish 7 strain of R. conorii. Splenocytes from these mice were analysed in the early stage of the infection by flow cytometry and compared with uninfected controls. Our results showed an increase in NKT cells in infected mice. Additionally, NKT interleukin (IL)-17(+) cells increased three days after infection, together with a concurrent decrease in the relative amount of NKT interferon (IFN)-\u3b3(+) cells. We also confirmed a higher amount of NK IFN-\u3b3(+) cells in infected mice. Taken together, our data showed that NKT cells producing Il-17 increased during the early stage of rickettsial infection. These results suggest a connection between IL-17(+) NKT cells and vasculitis, which is the main clinical symptom of rickettsiosi

Acute febrile illness is associated with Rickettsia spp infection in dogs

BACKGROUND: Rickettsia conorii is transmitted by Rhipicephalus sanguineus ticks and causes Mediterranean Spotted Fever (MSF) in humans. Although dogs are considered the natural host of the vector, the clinical and epidemiological significance of R. conorii infection in dogs remains unclear. The aim of this prospective study was to investigate whether Rickettsia infection causes febrile illness in dogs living in areas endemic for human MSF. METHODS: Dogs from southern Italy with acute fever (n = 99) were compared with case–control dogs with normal body temperatures (n = 72). Serology and real-time PCR were performed for Rickettsia spp., Ehrlichia canis, Anaplasma phagocytophilum/A. platys and Leishmania infantum. Conventional PCR was performed for Babesia spp. and Hepatozoon spp. Acute and convalescent antibodies to R. conorii, E. canis and A. phagocytophilum were determined. RESULTS: The seroprevalence rates at first visit for R. conorii, E. canis, A. phagocytophilum and L. infantum were 44.8%, 48.5%, 37.8% and 17.6%, respectively. The seroconversion rates for R. conorii, E. canis and A. phagocytophilum were 20.7%, 14.3% and 8.8%, respectively. The molecular positive rates at first visit for Rickettsia spp., E. canis, A. phagocytophilum, A. platys, L. infantum, Babesia spp. and Hepatozoon spp. were 1.8%, 4.1%, 0%, 2.3%, 11.1%, 2.3% and 0.6%, respectively. Positive PCR for E. canis (7%), Rickettsia spp. (3%), Babesia spp. (4.0%) and Hepatozoon spp. (1.0%) were found only in febrile dogs. The DNA sequences obtained from Rickettsia and Babesia PCRs positive samples were 100% identical to the R. conorii and Babesia vogeli sequences in GenBank®, respectively. Febrile illness was statistically associated with acute and convalescent positive R. conorii antibodies, seroconversion to R. conorii, E. canis positive PCR, and positivity to any tick pathogen PCRs. Fourteen febrile dogs (31.8%) were diagnosed with Rickettsia spp. infection based on seroconversion and/or PCR while only six afebrile dogs (12.5%) seroconverted (P = 0.0248). The most common clinical findings of dogs with Rickettsia infection diagnosed by seroconversion and/or PCR were fever, myalgia, lameness, elevation of C-reactive protein, thrombocytopenia and hypoalbuminemia. CONCLUSIONS: This study demonstrates acute febrile illness associated with Rickettsia infection in dogs living in endemic areas of human MSF based on seroconversion alone or in combination with PCR

Gastrointestinal nematode infections in German sheep

The objective of the present study was to determine the prevalence and variation of natural gastrointestinal nematode (GIN) infections in lambs according to birth type, gender and breed based on individual faecal egg counts (FEC) from various regions in Germany. A total of 3,924 lambs (3 to 15 months old) with different genetic backgrounds (Merinoland, German Blackhead Mutton, Rhoen, Texel and Merino long-wool) were individually sampled during the grazing period between 2006 and 2008. Furthermore, pooled faecal samples from each of the farms were cultured in order to differentiate the third-stage larvae of the nematode spp. Sixty-three percent of the lambs were infected with GIN. The infections were mostly low to moderate and involved several nematode species. The Trichostrongylus spp. was the predominant species based on the percentage of larvae in faecal cultures. Only 11.4% of the lambs were free of Eimeria oocysts. Tapeworm eggs were encountered in 13.2% of all samples. The prevalence of GIN infections varied significantly (P < 0.001) among farms. A significantly higher FEC (P < 0.05) was observed in multiple-born lambs when compared with singletons. Moreover, male lambs were more susceptible to infection than females (P < 0.001). No significant differences (P > 0.05) were observed between breeds regarding FEC. Inter-individual variations were higher than inter-breed differences, which may indicate the possibility of selection within these breeds for parasites resistance as described in earlier studies

VectAbundance: a spatio-temporal database of Aedes mosquitoes observations

Modelling approaches play a crucial role in supporting local public health agencies by estimating and forecasting vector abundance and seasonality. However, the reliability of these models is contingent on the availability of standardized, high-quality data. Addressing this need, our study focuses on collecting and harmonizing egg count observations of the mosquito&nbsp;Aedes albopictus, obtained through ovitraps in monitoring and surveillance efforts across Albania, France, Italy, and Switzerland from 2010 to 2022. We processed the raw observations to obtain a continuous time series of ovitraps observations allowing for an extensive geographical and temporal coverage of Ae. albopictus population dynamics. The resulting post-processed observations are stored in the open-access database VectAbundance.This initiative addresses the critical need for accessible, high-quality data, enhancing the reliability of modelling efforts and bolstering public health preparednes

A review on the eco-epidemiology and clinical management of human granulocytic anaplasmosis and its agent in Europe

Anaplasma phagocytophilum is the agent of tick-borne fever, equine, canine and human granulocytic anaplasmosis. The common route of A. phagocytophilum transmission is through a tick bite, the main vector in Europe being Ixodes ricinus. Despite the apparently ubiquitous presence of the pathogen A. phagocytophilum in ticks and various wild and domestic animals from Europe, up to date published clinical cases of human granulocytic anaplasmosis (HGA) remain rare compared to the worldwide status. It is unclear if this reflects the epidemiological dynamics of the human infection in Europe or if the disease is underdiagnosed or underreported. Epidemiologic studies in Europe have suggested an increased occupational risk of infection for forestry workers, hunters, veterinarians, and farmers with a tick-bite history and living in endemic areas. Although the overall genetic diversity of A. phagocytophilum in Europe is higher than in the USA, the strains responsible for the human infections are related on both continents. However, the study of the genetic variability and assessment of the difference of pathogenicity and infectivity between strains to various hosts has been insufficiently explored to date. Most of the European HGA cases presented as a mild infection, common clinical signs being pyrexia, headache, myalgia and arthralgia. The diagnosis of HGA in the USA was recommended to be based on clinical signs and the patient’s history and later confirmed using specialized laboratory tests. However, in Europe since the majority of cases are presenting as mild infection, laboratory tests may be performed before the treatment in order to avoid antibiotic overuse. The drug of choice for HGA is doxycycline and because of potential for serious complication the treatment should be instituted on clinical suspicion alone