Super-resolution imaging and estimation of protein copy numbers at single synapses with DNA-PAINT

In the brain, the strength of each individual synapse is defined by the complement of proteins present or the "local proteome." Activity-dependent changes in synaptic strength are the result of changes in this local proteome and posttranslational protein modifications. Although most synaptic proteins have been identified, we still know little about protein copy numbers in individual synapses and variations between synapses. We use DNA-point accumulation for imaging in nanoscale topography as a single-molecule super-resolution imaging technique to visualize and quantify protein copy numbers in single synapses. The imaging technique provides near-molecular spatial resolution, is unaffected by photobleaching, enables imaging of large field of views, and provides quantitative molecular information. We demonstrate these benefits by accessing copy numbers of surface AMPA-type receptors at single synapses of rat hippocampal neurons along dendritic segments

Sound modes broadening for Fibonacci one dimensional quasicrystals

We investigate vibrational excitation broadening in one dimensional Fibonacci model of quasicrystals (QCs). The chain is constructed from particles with two masses following the Fibonacci inflation rule. The eigenmode spectrum depends crucially on the mass ratio. We calculate the eigenstates and eigenfunctions. All calculations performed self-consistently within the regular expansion over the three wave coupling constant. The approach can be extended to three dimensional systems. We find that in the intermediate range of mode coupling constants, three-wave broadening for the both types of systems (1D Fibonacci and 3D QCs) depends universally on frequency. Our general qualitative conclusion is that for a system with a non-simple elementary cell phonon spectrum broadening is always larger than for a system with a primitive cell (provided all other characteristics are the same).Comment: 2o pages, 15 figure

Continuum elastic sphere vibrations as a model for low-lying optical modes in icosahedral quasicrystals

The nearly dispersionless, so-called "optical" vibrational modes observed by inelastic neutron scattering from icosahedral Al-Pd-Mn and Zn-Mg-Y quasicrystals are found to correspond well to modes of a continuum elastic sphere that has the same diameter as the corresponding icosahedral basic units of the quasicrystal. When the sphere is considered as free, most of the experimentally found modes can be accounted for, in both systems. Taking into account the mechanical connection between the clusters and the remainder of the quasicrystal allows a complete assignment of all optical modes in the case of Al-Pd-Mn. This approach provides support to the relevance of clusters in the vibrational properties of quasicrystals.Comment: 9 pages without figure

Metabolic rate of nocturnal incubation in female great tits, Parus major, in relation to clutch size measured in a natural environment

To study the energetic costs of incubation in relation to clutch size, clutch sizes were manipulated and the metabolic rate of female great tits, Parus major (Linnaeus), during nocturnal incubation (MRinc) was measured using mobile oxygen analysers. Individuals were measured on consecutive nights while incubating their own or manipulated clutches. The experiment was performed under field conditions in order to place possible effects of clutch size manipulation within the context of other factors explaining variation in MRinc. Females spent more energy when incubating enlarged clutches as compared with controls (6-10% more energy for three additional eggs) but did not spend significantly less energy when incubating reduced clutches. MRinc was strongly negatively related to ambient temperature. The effect of clutch enlargement is consistent with previous studies whereas the absence of an effect of clutch reduction is not. The small effect of clutch enlargement on MRinc highlights the need for further studies to include measurements of daily energy expenditure in order to judge how important energy expenditure can be in explaining fitness consequences of incubating experimentally enlarged clutches

Electronic structure of the (111) and (-1-1-1) surfaces of cubic BN: A local-density-functional ab initio study

We present ab initio local-density-functional electronic structure calculations for the (111) and (-1-1-1) surfaces of cubic BN. The energetically stable reconstructions, namely the N adatom, N3 triangle models on the (111), the (2x1), boron and nitrogen triangle patterns on the (-1-1-1) surface are investigated. Band structure and properties of the surface states are discussed in detail.Comment: 8 pages, 12 figure

Designing Secure Service Workflows in BPEL

This paper presents an approach that we have developed to support the design of secure service based applications in BPEL. The approach is based on the use of secure service composition patterns, which are proven to preserve composition level security properties if the services that are composed according to the pattern satisfy other properties individually. The secure service composition patterns are used for two purposes: (a) to analyse whether a given workflow fragment satisfies a given security property, and (b) to generate com-positions of services that could substitute for individual services within the workflow that cause the violation of the security properties. Our approach has been implemented in a tool that is based on Eclipse BPEL Designer

Quantum Particles Constrained on Cylindrical Surfaces with Non-constant Diameter

We present a theoretical formulation of the one-electron problem constrained on the surface of a cylindrical tubule with varying diameter. Because of the cylindrical symmetry, we may reduce the problem to a one-dimensional equation for each angular momentum quantum number $m$ along the cylindrical axis. The geometrical properties of the surface determine the electronic structures through the geometry dependent term in the equation. Magnetic fields parallel to the axis can readily be incorporated. Our formulation is applied to simple examples such as the catenoid and the sinusoidal tubules. The existence of bound states as well as the band structures, which are induced geometrically, for these surfaces are shown. To show that the electronic structures can be altered significantly by applying a magnetic field, Aharonov-Bohm effects in these examples are demonstrated.Comment: 7 pages, 7 figures, submitted to J. Phys. Soc. Jp

VINYL: The VIrtual Neutron and x-raY Laboratory and its applications

Experiments conducted in large scientific research infrastructures, such as synchrotrons, free electron lasers and neutron sources become increasingly complex. Such experiments, often investigating complex physical systems, are usually performed under strict time limitations and may depend critically on experimental parameters. To prepare and analyze these complex experiments, a virtual laboratory which provides start-to-end simulation tools can help experimenters predict experimental results under real or close to real instrument conditions. As a part of the PaNOSC (Photon and Neutron Open Science Cloud) project, the VIrtual Neutron and x-raY Laboratory (VINYL) is designed to be a cloud service framework to implement start-to-end simulations for those scientific facilities. In this paper, we present an introduction of the virtual laboratory framework and discuss its applications to the design and optimization of experiment setups as well as the estimation of experimental artifacts in an X-ray experiment