The use of microarrays for the identification of the origin of genes of avian influenza viruses in wild birds

Forty-two strains of avian influenza viruses were isolated from the wild waterfowls’ feces in the city of Moscow. These viruses, as well as reference strains and some experimental reassortants, were analyzed by microarrays. The microarrays contained 176 probes to the different segments of influenza virus genome. The microarray helps to determine 1) the hemagglutinin and neuraminidase proteins subtype; 2) the primary structure of the C-terminal sequence of the viral NS1 protein, which serves as a ligand for the PDZ domain; 3) the presence of stop codons in the reading frame of PB1-F2 as well as the N66S substitution in the PB1-F2 viral protein; 4) the presence of the polybasic site for hemagglutinin cleavage. The viruses of the H3N1, H3N6, H3N8, H4N6, H1N1, H5N3, and H11N9 subtypes were identified from the group of wild birds’ isolates. All isolates contained the ESEV sequence at the C-terminus of the NS1 protein and the full-length reading frame for the PB1-F2 protein. The replacement of N66S in PB1-F2 was found in six strains. However, the presence of the ESEV sequence (ligand of PDZ domain) in the NS1 virus protein and the N66S substitution in PB1-F2 did not lead to the pathogenicity of these viruses for mice. All isolates demonstrated high yield growth in chicken embryos and were infectious and immunogenic for mice, but did not induce any clinical symptoms.Forty-two strains of avian influenza viruses were isolated from the wild waterfowls’ feces in the city of Moscow. These viruses, as well as reference strains and some experimental reassortants, were analyzed by microarrays. The microarrays contained 176 probes to the different segments of influenza virus genome. The microarray helps to determine 1) the hemagglutinin and neuraminidase proteins subtype; 2) the primary structure of the C-terminal sequence of the viral NS1 protein, which serves as a ligand for the PDZ domain; 3) the presence of stop codons in the reading frame of PB1-F2 as well as the N66S substitution in the PB1-F2 viral protein; 4) the presence of the polybasic site for hemagglutinin cleavage. The viruses of the H3N1, H3N6, H3N8, H4N6, H1N1, H5N3, and H11N9 subtypes were identified from the group of wild birds’ isolates. All isolates contained the ESEV sequence at the C-terminus of the NS1 protein and the full-length reading frame for the PB1-F2 protein. The replacement of N66S in PB1-F2 was found in six strains. However, the presence of the ESEV sequence (ligand of PDZ domain) in the NS1 virus protein and the N66S substitution in PB1-F2 did not lead to the pathogenicity of these viruses for mice. All isolates demonstrated high yield growth in chicken embryos and were infectious and immunogenic for mice, but did not induce any clinical symptoms

Thermal fluctuations and longitudinal relaxation of single-domain magnetic particles at elevated temperatures

We present numerical and analytical results for the swiching times of magnetic nanoparticles with uniaxial anisotropy at elevated temperatures, including the vicinity of T_c. The consideration is based in the Landau-Lifshitz-Bloch equation that includes the relaxation of the magnetization magnitude M. The resulting switching times are shorter than those following from the naive Landau-Lifshitz equation due to (i) additional barrier lowering because of the reduction of M at the barrier and (ii) critical divergence of the damping parameters.Comment: 4 PR pages, 1 figur

TECHNOLOGY FOR PRODUCING IRON CASTINGS WITH A HARD WEAR-RESISTANT AND IMPACT-RESISTANT VISCOUS PART OF A MELT

В работе предложена и исследована технология получения чугунных отливок с дифференцированной структурой и свойствами обеспечивающая формирование рабочего слоя изделия из твердого износостойкого белого чугуна, а монтажной части – из вязкого ударостойкого высокопрочного чугуна с шаровидным графитом. Многочисленными лабораторными исследованиями с использованием методов физического и компьютерного моделирования, а также на натурных отливках подтверждена возможность реализации предлагаемой технологии, которая предназначается для изготовления деталей, работающих в условиях ударно-абразивного износа.The work proposed and studied technology for producing iron castings with differentiated structure and properties ensuring the formation of the working layer of wear-resistant products made of hard white iron, and mounting parts – impact-resistant high-strength ductile cast iron with nodular graphite. Numerous laboratory research using the methods of physical and computer simulation as well as natural castings confirmed the feasibility of the proposed technology, which is intended for parts operating under impact-abrasive wear

DEVELOPMENT OF RECIPE AND TECHNOLOGY OF COMBINEDSEMI-FINISHED PRODUCTS

The authors set the goal - to develop a formulation of semi-finished meat and vegetable horse meat, poultry, minced eggplant. Compiled by experienced formulation of model samples of meat and vegetable raw materials. In model samples of minced meat the pH was studied, organoleptic characteristics and output were determined in the finished experimental products. As a result of experimental studies, the recipe of meat and cereal cutlets with the optimal ratio of raw materials has been established

Generalised Kawada-Satake method for Mackey functors in class field theory

We propose and study a generalised Kawada--Satake method for Mackey functors in the class field theory of positive characteristic. The root of this method is in the use of explicit pairings, such as the Artin--Schreier--Witt pairing, for groups describing abelian extensions. We separate and simplify the algebraic component of the method and discuss a relation between the existence theorem in class field theory and topological reflexivity with respect to the explicit pairing. We apply this method to derive higher local class field theory of positive characteristic, using advanced properties of topological Milnor K-groups of such fields

Control of the chirality and polarity of magnetic vortices in triangular nanodots

Magnetic vortex dynamics in lithographically prepared nanodots is currently a subject of intensive research, particularly after recent demonstration that the vortex polarity can be controlled by in-plane magnetic field. This has stimulated the proposals of non-volatile vortex magnetic random access memories. In this work, we demonstrate that triangular nanodots offer a real alternative where vortex chirality, in addition to polarity, can be controlled. In the static regime, we show that vortex chirality can be tailored by applying in-plane magnetic field, which is experimentally imaged by means of Variable-Field Magnetic Force Microscopy. In addition, the polarity can be also controlled by applying a suitable out-of-plane magnetic field component. The experiment and simulations show that to control the vortex polarity, the out-of-plane field component, in this particular case, should be higher than the in-plane nucleation field. Micromagnetic simulations in the dynamical regime show that the magnetic vortex polarity can be changed with short-duration magnetic field pulses, while longer pulses change the vortex chirality.Comment: 18 pages, 11 figure

Influence of clinical and biological variants of premature aging on cognitive functionality

To study the features of cognitive functioning of people with various clinical and biological variants of premature aging. At the same time, insufficient attention is paid to cognitive and psychological functionality, which is largely the basis for the formation of resilienc

Direct observation of transverse and vortex metastable magnetic domains in cylindrical nanowires

We present experimental evidence of transverse magnetic domains, previously observed only in nanostrips, in CoNi cylindrical nanowires with designed crystal symmetry and tailored magnetic anisotropy. The transverse domains are found together with more conventional vortex domains along the same cylindrical nanowire, denoting a bistable system with similar energies. The surface and the inner magnetization distribution in both types of domains are analyzed by photoemission electron microscopy with x-ray magnetic circular dichroism contrast, and hysteresis loop in individual nanowires are measured by magneto-optical Kerr effect. These experimental data are understood and compared with complementary micromagnetic simulations