Filamin cross-linked semiflexible networks: Fragility under strain

The semiflexible F-actin network of the cytoskeleton is cross-linked by a variety of proteins including filamin, which contain Ig-domains that unfold under applied tension. We examine a simple semiflexible network model cross-linked by such unfolding linkers that captures the main mechanical features of F-actin networks cross-linked by filamin proteins and show that under sufficiently high strain the network spontaneously self-organizes so that an appreciable fraction of the filamin cross-linkers are at the threshold of domain unfolding. We propose an explanation of this organization based on a mean-field model and suggest a qualitative experimental signature of this type of network reorganization under applied strain that may be observable in intracellular microrheology experiments of Crocker et al.Comment: 4 Pages, 3 figures, Revtex4, submitted to PR

Unfolding cross-linkers as rheology regulators in F-actin networks

We report on the nonlinear mechanical properties of a statistically homogeneous, isotropic semiflexible network cross-linked by polymers containing numerous small unfolding domains, such as the ubiquitous F-actin cross-linker Filamin. We show that the inclusion of such proteins has a dramatic effect on the large strain behavior of the network. Beyond a strain threshold, which depends on network density, the unfolding of protein domains leads to bulk shear softening. Past this critical strain, the network spontaneously organizes itself so that an appreciable fraction of the Filamin cross-linkers are at the threshold of domain unfolding. We discuss via a simple mean-field model the cause of this network organization and suggest that it may be the source of power-law relaxation observed in in vitro and in intracellular microrheology experiments. We present data which fully justifies our model for a simplified network architecture.Comment: 11 pages, 4 figures. to appear in Physical Review

Discovery of the Pre-Main Sequence Population of the Stellar Association LH 95 in the Large Magellanic Cloud with Hubble Space Telescope ACS Observations

We report the discovery of an extraordinary number of pre-main sequence (PMS) stars in the vicinity of the stellar association LH 95 in the Large Magellanic Cloud (LMC). Using the {\em Advanced Camera for Surveys} on-board the {\em Hubble} Space Telescope in wide-field mode we obtained deep high-resolution imaging of the main body of the association and of a nearby representative LMC background field. These observations allowed us to construct the color-magnitude diagram (CMD) of the association in unprecedented detail, and to decontaminate the CMD for the average LMC stellar population. The most significant result is the direct detection of a substantial population of PMS stars and their clustering properties with respect to the distribution of the higher mass members of the association. Although LH 95 represents a rather modest star forming region, our photometry, with a detection limit $V$ \lsim 28 mag, reveals in its vicinity more than 2,500 PMS stars with masses down to $\sim 0.3$ M{\solar}. Our observations offer, thus, a new perspective of a typical LMC association: The stellar content of LH 95 is found to extend from bright OB stars to faint red PMS stars, suggesting a fully populated Initial Mass Function (IMF) from the massive blue giants down to the sub-solar mass regime.Comment: Accepted for Publication in ApJ Letters - 4 Pages ApJ paper format - 3 figures in low-resolution/grayscal

Structures in surface-brightness profiles of LMC and SMC star clusters: evidence of mergers?

The LMC and SMC are rich in binary star clusters, and some mergers are expected. It is important to characterize single clusters, binary clusters and candidates to mergers. We selected a sample of star clusters in each Cloud with this aim. Surface photometry of 25 SMC and 22 LMC star clusters was carried with the ESO Danish 1.54 m telescope. 23 clusters were observed for the first time for these purposes. We fitted Elson, Fall and Freeman (1987, EFF) profiles to the data, deriving structural parameters, luminosities and masses. We also use isophotal maps to constrain candidates to cluster interactions.} {The structural parameters, luminosities and masses presented good agreement with those in the literature. Three binary clusters in the sample have a double profile. Four clusters (NGC 376, K 50, K 54 and NGC 1810) do not have companions and present as well important deviations from EFF profiles. The present sample contains blue and red Magellanic clusters. Extended EFF profiles were detected in some blue clusters. We find evidence that important deviations from the body of EFF profiles might be used as a tool to detect cluster mergers.Comment: 16 pages and 8 figures. Accepted by A&