Musicoterapia en una Unidad de Cuidados Intensivos Neonatales: experiencia benéfica para el binomio

ResumenIntroducciónDesde hace varios años se está utilizando la música como herramienta terapéutica y preventiva en la neonatología, se han documentado en un gran número de artículos de investigación, sin embargo, en México esta técnica es poco utilizada.ObjetivoMejorar las condiciones de los neonatos atendidos en una Unidad de Cuidados Intensivos (UCIN) y sus familiares, mediante el uso de musicoterapia, para minimizar el estrés y los daños colaterales.Materiales y métodosUn grupo de 2 neonatólogos, 3 residentes de pediatría, 10 enfermeras y un concertista profesional de violín, llevaron a cabo 6 sesiones musicales semanales en la Unidad de Cuidados Intensivos Neonatales Externos del Hospital Civil de Guadalajara. Durante el concierto se valoró individualmente a cada neonato, registrando los signos vitales por monitorización electrónica, además se registró el estado anímico de los familiares.ResultadosDurante las sesiones de música los neonatales no presentaron cambios significativos en sus constantes vitales, en el personal de la UCIN y en los padres de recién nacidos hospitalizados se vieron efectos benéficos, refiriendo disminución del estrés y altos índices de satisfacción.ConclusionesLa música puede tener un efecto positivo como terapia coadyuvante en neonatos de alto riesgo, principalmente en prematuros. Se recomienda difundir y aplicar esta metodología en las UCIN del país.AbstractIntroductionSince several years ago, music has been used as a therapeutic and preventive tool in neonatology. A great number of research papers have been written on the subject; however, this technique is rarely used in Mexico.ObjectiveTo improve the condition of neonates at Neonatal Intensive Care Units (NICU) and their relatives, in order to minimize any stress and collateral damage.Material and methods: A group of two neonatologists, 3 residents, 10 nurses and a professional concert violinist, conducted six weekly music sessions in the Outpatient Neonatal Intensive Care Unit (ONICU) of our institution. During the concert, we individually assessed each newborn, recording vital signs through electronic monitoring; additionally, we recorded the mood of any other family members present.ResultsDuring the music sessions, the neonatal patients showed no significant changes in vital signs; yet, ONICU staff and the parents/relatives of hospitalized newborns showed beneficial effects, reporting decreased stress and high levels of satisfaction.ConclusionsMusic can have a positive effect as adjunctive therapy in high-risk newborns, especially in premature babies. We therefore recommend applying this methodology in the ONICU

Eosinophilic peritonitis in a patient under continuous ambulatory peritoneal dialysis successfully treated with steroids

A 28 year-old woman with chronic kidney disease stage 5 and hemodialysis in 2007. She received a first kidney transplant from related live donor and suffered chronic graft rejection returning to hemodialysis in 2011. In 2015, the patient entered to continuous ambulatory peritoneal dialysis and ten days after the placement of the catheter, while she was in the training of the technique of peritoneal dialysis, she presented a murky dialysis fluid, without an abdominal pain or fever, with eosinophilia in the peritoneal fluid and peripheral blood. She received prednisone with resolution of the problem.Mujer de 28 años de edad con enfermedad renal crónica en estadio 5 y en hemodiálisis desde el 2007. Recibió un primer trasplante renal de donante vivo relacionado, del cual sufrió rechazo crónico del injerto, y retornó a hemodiálisis en el 2011. En el 2015, la paciente ingresó a diálisis peritoneal continua ambulatoria y diez días después de la colocación del catéter, mientras se encontraba en entrenamiento de la técnica de diálisis peritoneal, presentó líquido de diálisis turbio, sin dolor abdominal ni fiebre, y con eosinofilia en el líquido ascítico y en sangre periférica. Recibió prednisona y se resolvió el cuadro

In silico and In vitro analysis of MAP3773c protein from Mycobacterium avium subsp. Paratuberculosis

Paratuberculosis is a disease caused by Mycobacterium avium subsp. paratuberculosis (MAP). It is of great interest to better understand the proteins involved in the pathogenicity of this organism in order to be able to identify potential therapeutic targets and design new vaccines. The protein of interest–MAP3773c–was investigated, and molecular modeling in silico, docking, cloning, expression, purification, and partial characterization of the recombinant protein were achieved. In the in silico study, it was shown that MAP3773c of MAP has 34% sequence similarity with Mycobacterium tuberculosis (MTB) FurB, which is a zinc uptake regulator (Zur) protein. The docking data showed that MAP3773c exhibits two metal-binding sites. The presence of structural Zn2+ in the purified protein was confirmed by SDS-PAGE PAR staining. The purification showed one band that corresponded to a monomer, which was confirmed by liquid chromatography–mass spectrometry (LC-MS). The presence of a monomer was verified by analyzing the native protein structure through BN-SDS-PAGE (Native Blue (BN) Two-Dimensional Electrophoresis) and BN–Western blotting. The MAP3773c protein contains structural zinc. In conclusion, our results show that MAP3773c displays the features of a Fur-type protein with two metal-binding sites, one of them coordinating structural Zn2+

The Ferric uptake regulator (Fur) and iron availability control the production and maturation of the antibacterial peptide microcin E492

© 2018 Marcoleta et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Microcin E492 is a pore-forming bacteriocin with toxic activity against Enterobacteriaceae, which undergoes amyloid aggregation as a mechanism to regulate its toxicity. To be active, it requires the posttranslational attachment to the C-terminus of a glycosylated enterochelin derivative (salmochelin), a process carried out by the proteins MceC, MceI and MceJ encoded in the MccE492 gene cluster. Both microcin E492 and salmochelin have a proposed role in the virulence of the bacterial pathogen Klebsiella pneumoniae. Besides, enterochelin is produced as a response to low iron availability and its synthesis is controlled by the global iron regulator Fur. Since the production of active microcin E492 depends on enterochelin biosynthesi

Optimization of castor seed oil extraction process using response surface methodology

This work focuses on the study of the oil extraction yield from castor seed using three different seed conditions: whole, minced and bare endosperm. Taguchi design was used to determine the contribution of the following parameters: seed condition, seed load in the extractor, temperature, and pressure. It was proved that it is necessary to introduce the whole seed and that the presence of the pericarp increases the extraction yield. The contribution of the control factors has an extraction yield limit. After determining which factors contributed to the process, these were left at their optimum levels aiming to reduce the control factors to only two. The complete analysis was done using a surface response methodology giving the best parameter for temperature and pressure that allows a better yielding mechanical extraction. The oil extraction yield can be kept up to 35% of the seed

MceX overexpression reduces the antibacterial activity of <i>E</i>. <i>coli</i> cells carrying the gene cluster for microcin E492 production.

<p><i>E</i>. <i>coli</i> cells carrying pMccE492 (wt, in yellow) or pJAM434 (poor producing, in green) were transformed with pT5-<i>mceX</i> (driving overexpression of MceX) or pUC57 (control). Antibacterial activity was measured as a function of the growth inhibition halo’s area over a sensitive strain layer, in M9 medium supplemented with IPTG, and was normalized to the value obtained in the control condition for each case. Error bars indicate standard deviation from 40 measurements performed for each condition. **<i>P</i><0.01, ns: not significant.</p

List and sequence of primers used in this work.

<p>List and sequence of primers used in this work.</p