1,108 research outputs found
Wormholes, Gamma Ray Bursts and the Amount of Negative Mass in the Universe
In this essay, we assume that negative mass objects can exist in the
extragalactic space and analyze the consequences of their microlensing on light
from distant Active Galactic Nuclei. We find that such events have very similar
features to some observed Gamma Ray Bursts and use recent satellite data to set
an upper bound to the amount of negative mass in the universe.Comment: Essay awarded ``Honorable Mention'' in the Gravity Foundation
Research Awards, 199
Robustness and modularity properties of a non-covalent DNA catalytic reaction
The biophysics of nucleic acid hybridization and strand displacement have been used for the rational design of a number of nanoscale structures and functions. Recently, molecular amplification methods have been developed in the form of non-covalent DNA catalytic reactions, in which single-stranded DNA (ssDNA) molecules catalyze the release of ssDNA product molecules from multi-stranded complexes. Here, we characterize the robustness and specificity of one such strand displacement-based catalytic reaction. We show that the designed reaction is simultaneously sensitive to sequence mutations in the catalyst and robust to a variety of impurities and molecular noise. These properties facilitate the incorporation of strand displacement-based DNA components in synthetic chemical and biological reaction networks
Global embedding of the Kerr black hole event horizon into hyperbolic 3-space
An explicit global and unique isometric embedding into hyperbolic 3-space,
H^3, of an axi-symmetric 2-surface with Gaussian curvature bounded below is
given. In particular, this allows the embedding into H^3 of surfaces of
revolution having negative, but finite, Gaussian curvature at smooth fixed
points of the U(1) isometry. As an example, we exhibit the global embedding of
the Kerr-Newman event horizon into H^3, for arbitrary values of the angular
momentum. For this example, considering a quotient of H^3 by the Picard group,
we show that the hyperbolic embedding fits in a fundamental domain of the group
up to a slightly larger value of the angular momentum than the limit for which
a global embedding into Euclidean 3-space is possible. An embedding of the
double-Kerr event horizon is also presented, as an example of an embedding
which cannot be made global.Comment: 16 pages, 13 figure
Telavancin activity when tested by a revised susceptibility testing method against uncommonly isolated Gram-positive pathogens responsible for documented infections in hospitals worldwide (2011–2013)
AbstractThe broth microdilution method for telavancin susceptibility testing was revised and now utilises DMSO as solvent for stock solution preparation and diluent for stock solution dilution, following CLSI guidelines for water-insoluble agents. The revised method also incorporates polysorbate 80 in the test medium to mitigate drug binding to plastics. This revised methodology provides more accurate and reproducible MIC determinations, which results in values lower than the previously established method. This study was conducted to re-establish telavancin potencies and susceptibility profiles (using updated interpretive criteria) against a collection of uncommon clinical pathogens (3821 isolates). Telavancin showed MIC50 values of 0.06mg/L against tested staphylococcal species (MIC50/90, 0.03/0.06mg/L; 98.1–100.0% susceptible), with lower results for Staphylococcus hominis (MIC50, ≤0.015mg/L), Staphylococcus lugdunensis (MIC50, ≤0.015mg/L) and Staphylococcus simulans (MIC50, 0.03mg/L). Vancomycin (MIC50, 1mg/L), daptomycin (MIC50, 0.12–1mg/L) and linezolid (MIC50, 0.25–1mg/L) had MIC50 results at least four-fold higher than telavancin against CoNS. Streptococci (99.2–100.0% susceptible) displayed telavancin MIC50 values of ≤0.015–0.03mg/L. Vancomycin (MIC50, 0.25–0.5mg/L) and linezolid (MIC50, 0.5–1mg/L) had higher MIC50 results against streptococci, whilst daptomycin MIC50 values varied from ≤0.06mg/L to 0.5mg/L. Micrococcus, Listeria and Corynebacterium spp. were inhibited by telavancin at ≤0.015, ≤0.03 and ≤0.06mg/L, respectively. Telavancin exhibited potent in vitro activity against this collection, greater than comparators (daptomycin, linezolid, vancomycin). This study provides new baseline MIC results for telavancin and confirms the spectrum and potency of telavancin against less commonly encountered Gram-positive species
Meson Mass Splittings in the Nonrelativistic Model
Mass splittings between isodoublet meson pairs and between and
mesons of the same valence quark content are computed in a detailed
nonrelativistic model. The field theoretic expressions for such splittings are
shown to reduce to kinematic and Breit-Fermi terms in the nonrelativistic
limit. Algebraic results thus obtained are applied to the specific case of the
linear-plus-Coulomb potential, with resultant numbers compared to experiment.Comment: 29 pages with 2 tables and 4 figures, LBL-32872 and UCB-PTH-92/3
DNA hybridization catalysts and catalyst circuits
Practically all of life's molecular processes, from chemical synthesis to replication, involve enzymes that carry out their functions through the catalysis of metastable fuels into waste products. Catalytic control of reaction rates will prove to be as useful and ubiquitous in
DNA nanotechnology as it is in biology. Here we present experimental results on the control of the decay rates of a metastable DNA "fuel". We show that the fuel complex can be induced to decay with a rate about 1600 times faster than it would decay spontaneously. The original DNA hybridization catalyst [15] achieved a maximal speed-up of roughly 30. The fuel complex discussed here can therefore serve as the basic ingredient for an improved DNA hybridization catalyst. As an example application for DNA hybridization catalysts, we propose a method for implementing arbitrary digital logic circuits
A stitch in time: Efficient computation of genomic DNA melting bubbles
Background: It is of biological interest to make genome-wide predictions of
the locations of DNA melting bubbles using statistical mechanics models.
Computationally, this poses the challenge that a generic search through all
combinations of bubble starts and ends is quadratic.
Results: An efficient algorithm is described, which shows that the time
complexity of the task is O(NlogN) rather than quadratic. The algorithm
exploits that bubble lengths may be limited, but without a prior assumption of
a maximal bubble length. No approximations, such as windowing, have been
introduced to reduce the time complexity. More than just finding the bubbles,
the algorithm produces a stitch profile, which is a probabilistic graphical
model of bubbles and helical regions. The algorithm applies a probability peak
finding method based on a hierarchical analysis of the energy barriers in the
Poland-Scheraga model.
Conclusions: Exact and fast computation of genomic stitch profiles is thus
feasible. Sequences of several megabases have been computed, only limited by
computer memory. Possible applications are the genome-wide comparisons of
bubbles with promotors, TSS, viral integration sites, and other melting-related
regions.Comment: 16 pages, 10 figure
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