BLENDS OF POLY-3-HYDROXYBUTYRATE AND POLY-ε-CAPROLACTONE:MORPHOLOGY, PROPERTIES AND BIOCOMPATIBILITY

The use of biocompatible and biodegradable polymers is essential for modern biomedicine. One of the approaches to improving the mechanical properties of pure polymers is to obtain mixtures based on them.This work was carried within the state assignment of the Ministry of Science and Higher Education of the Russian Federation, project No. FEFE-2020-0015

Combination of CAKUT-syndrome and axial skeletal abnormalities

Combined anomalies of the urinary system and axial skeleton, including an L-shaped kidney, obstruction of the ureteropelvic junction (CAKUT syndrome, congenital anomalies of the kidney and urinary tract syndrome) and spinal anomalies, are extremely rare. The clinical case gives a description of a similar combined developmental anomaly

Robust Target Gene Discovery through Transcriptome Perturbations and Genome-Wide Enhancer Predictions in Drosophila Uncovers a Regulatory Basis for Sensory Specification

CisTarget X is a novel computational method that accurately predicts Atonal governed regulatory networks in the retina of the fruit fly

A comparison of the radiosensitisation ability of 22 different element metal oxide nanoparticles using clinical megavoltage X-rays

Background: A wide range of nanoparticles (NPs), composed of different elements and their compounds, are being developed by several groups as possible radiosensitisers, with some already in clinical trials. However, no systematic experimental survey of the clinical X-ray radiosensitising potential of different element nanoparticles has been made. Here, we directly compare the irradiation-induced (10 Gy of 6-MV X-ray photon) production of hydroxyl radicals, superoxide anion radicals and singlet oxygen in aqueous solutions of the following metal oxide nanoparticles: Al2O3, SiO2, Sc2O3, TiO2, V2O5, Cr2O3, MnO2, Fe3O4, CoO, NiO, CuO, ZnO, ZrO2, MoO3, Nd2O3, Sm2O3, Eu2O3, Gd2O3, Tb4O7, Dy2O3, Er2O3 and HfO2. We also examine DNA damage due to these NPs in unirradiated and irradiated conditions. Results: Without any X-rays, several NPs produced more radicals than water alone. Thus, V2O5 NPs produced around 5-times more hydroxyl radicals and superoxide radicals. MnO2 NPs produced around 10-times more superoxide anions and Tb4O7 produced around 3-times more singlet oxygen. Lanthanides produce fewer hydroxyl radicals than water. Following irradiation, V2O5 NPs produced nearly 10-times more hydroxyl radicals than water. Changes in radical concentrations were determined by subtracting unirradiated values from irradiated values. These were then compared with irradiation-induced changes in water only. Irradiation-specific increases in hydroxyl radical were seen with most NPs, but these were only significantly above the values of water for V2O5, while the Lanthanides showed irradiation-specific decreases in hydroxyl radical, compared to water. Only TiO2 showed a trend of irradiation-specific increase in superoxides, while V2O5, MnO2, CoO, CuO, MoO3 and Tb4O7 all demonstrated significant irradiation-specific decreases in superoxide, compared to water. No irradiation-specific increases in singlet oxygen were seen, but V2O5, NiO, CuO, MoO3 and the lanthanides demonstrated irradiation-specific decreases in singlet oxygen, compared to water. MoO3 and CuO produced DNA damage in the absence of radiation, while the highest irradiation-specific DNA damage was observed with CuO. In contrast, MnO2, Fe3O4 and CoO were slightly protective against irradiation-induced DNA damage. Conclusions: Beyond identifying promising metal oxide NP radiosensitisers and radioprotectors, our broad comparisons reveal unexpected differences that suggest the surface chemistry of NP radiosensitisers is an important criterion for their success

Разработка и изучение отраслевого стандартного образца активности вакцины против краснухи

Presents information on 1st series of branch standard sample to characterization the quality of the candidate of branch standard sample tested for performance evaluation: specific activity of authenticity, a description of sterility, presence of mycoplasma, loss on drying, pH, filling accuracy, the residual oxygen content in the vials. Attested feature of the new series of the standard sample is a specific activity, because the purpose of the of branch reference standard activity live Rubella vaccine is the suitability of the results of determining the specific activity of the Rubella virus in vaccines. Indicator specific activity of the candidate in the of branch reference standard was assessed by interlaboratory studies compared to current 1st standard sample of the enterprise and 1st International Reference Reagent For Rubella (Live) NIBSC-91/688. Certified value of the index is set «Specific activity of branch reference standard activity for rubella (4,63±0,50) lgCCE50/0,5 ml. Shelf-life stated by using test accelerated the degradation is not less than at minus 20°C.Представлены материалы по аттестации первой серии отраслевого стандартного образца (ОСО) активности вакцины против краснухи. Для характеристики качества кандидата в ОСО проведены испытания по оценке показателей: специфическая активность, подлинность, описание, стерильность, присутствие микоплазм, потеря в массе при высушивании, рН, точность наполнения, остаточное содержание кислорода в ампулах. Аттестуемой характеристикой стандартного образца является специфическая активность. Назначение ОСО активности вакцины против краснухи - это оценка приемлемости результатов определения активности вируса краснухи в вакцинах. Показатель «Специфическая активность» кандидата в ОСО оценивали по результатам межлабораторных исследований в сравнении со стандартным образцом предприятия и международным стандартом: 1st International Reference Reagent For Rubella (Live) NIBSC-91/688. Установлено аттестованное значение показателя «Специфическая активность» ОСО активности вакцины против краснухи: (4,63±0,50)lg ТЦД50/0,5 мл. Срок годности, установленный с помощью теста ускоренного старения, при условии хранения при минус 20°С, - не менее 5 лет. Результаты мониторинга стабильности ОСО подтверждают его стабильность за период наблюдения

Phytoplankton responses to marine climate change – an introduction

Phytoplankton are one of the key players in the ocean and contribute approximately 50% to global primary production. They serve as the basis for marine food webs, drive chemical composition of the global atmosphere and thereby climate. Seasonal environmental changes and nutrient availability naturally influence phytoplankton species composition. Since the industrial era, anthropogenic climatic influences have increased noticeably – also within the ocean. Our changing climate, however, affects the composition of phytoplankton species composition on a long-term basis and requires the organisms to adapt to this changing environment, influencing micronutrient bioavailability and other biogeochemical parameters. At the same time, phytoplankton themselves can influence the climate with their responses to environmental changes. Due to its key role, phytoplankton has been of interest in marine sciences for quite some time and there are several methodical approaches implemented in oceanographic sciences. There are ongoing attempts to improve predictions and to close gaps in the understanding of this sensitive ecological system and its responses

Alprazolam therapy effects on quality of life and vegetative dysfunction syndrome in patients with idiopathic mitral valve prolapse

Aim. To assess quality of life (QoL) and severity of vegetative dysfunction syndrome (VDS) in patients with idiopathic mitral valve prolapse (MVP); to evaluate the effects of alprazolam therapy on QoL and VDS. Material and methods. This single-blind, placebo-controlled study included 60 patients with idiopathic MVP (33,3% men, 66,7% women). All patients were randomised into two groups: the main group (MG), receiving alprazolam, and the control group (CG), receiving placebo. Both groups were comparable by age (mean age 30,8±0,4 and 31,1±0,2 years, respectively) and gender structure. In all patients with idiopathic MVP, organic internal pathology was ruled out. Both groups underwent complex examination at baseline and 10 weeks after the therapy started. Results. Clinically significant effectiveness of the treatment (VDS severity reduction (in points) by at least 50%, comparing to the baseline level) was observed among 80,0% of the patients receiving alprazolam (71,4% men, 82,6% women) and only in 33,3% (58,3% men, 16,7% women) of the controls. Alprazolam was clinically effective by the “DISS-work” scale in 30% (28,6% men, 30,4% women), by the “social life” scale – in 43,3% (57,1% men, 39,1% women), and by the “personal relationships” scale – in 56,7% (42,8% men, 60,9% women). In the placebo group, the respective percentages were 43,3% (50% men, 38,9% women), 30,0% (41,7% men, 22,2% women), and 46,7% (58,3% men, 38,9% women). Conclusion. Alprazolam therapy demonstrated significant QoL improvement and VDS severity reduction in patients with MVP