62 research outputs found
Comparison of titanium mesh implants with PLA-hydroxyapatite coatings for maxillofacial cancer reconstruction
Since 2013 physics of TPU and oncologists from the TCRI with participation of the βConMetβ company (Moscow) and the βSintelβ company (Tomsk Special Economic Zone resident) have been working on the theme entitled βDevelopment of the composite implants for reconstructive surgery of a craniofacial areas of the traumatological and oncological patientsβ supported with the Federal Program βR&D, part 1.3β. The goal was to develop the maxillo-facial implants on the basis of the transformable titanium mesh with PLA & hydroxyapatite coating. According to the Contract No. 14.578.21.0031, the team of developers had to start supplying these advanced implants to the industrial partners up to 2017. This research was supported with the preliminary market researches by the ISPMS SB RAS and the TP βMFβ. The stages of preliminary market researches were: 1) research of the Worldwide CMF market; 2) forecasting the BRIC CMF market up to 2020; 3) the total Russian market (epidemiology) estimation as a sum of official calculations and statistics; 4) looking for the best foreign analogue prices, comparing their and our implant properties; 5) search for the best Russian analogues; 6) the investigation of the world patent databaseEspacenet for the last years, and finding the owners and applicants of patents of CMF osteosynthesis plates on the basis of titanium coated with PLA & hydroxyapatite; 7) comparison of the domestic implants, and making conclusions. Several variants of the meshes have got the equal quality with the best foreign and Russian implants. The closest analogues were titanium, polyethylene, PEEK composite meshes suited to the patient shape by the Synthes company in 2014, and the only hybrid titanium "Grey" implant with layers of gelatin, dextran, collagen, HAP & BMP-2 was found. This implant was produced by Russian institution, and it was mentioned in the report on clinical trials by L.A. Pavlova et al., 2014 [1]. There are no manufacturers of the coated implants in Russia. The average price of the similar foreign implants varies from 12 up to 4
Reconstructive surgery for oral cavity cancer
Treatment of patients with advanced oral cavity cancer remains challenging
ΠΡΠΎΠ³Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΎΡΠ΄Π°Π»Π΅Π½Π½ΡΡ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΎΠ² ΠΏΡΠΈ ΡΠ°ΠΊΠ΅ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ
Background. Distant organ tumor dissemination is a major cause of breast cancer-related deaths. Breast cancer can metastasize to several organs, and the most frequent metastatic sites include the bones, lungs and liver. There is a question what factors can influence the direction of spread of tumor cells to a particular organ.Material and Methods. We summarized the data available in the world literature on methods for prediction of the localization of distant metastases in breast cancer patients.Results. We divided the factors associated with the localization of distant metastases into two main groups: clinicopathological parameters of breast cancer patients and molecular features of tumor microenvironment and tumor cells (primary tumor and circulating tumor cells) or its derivates β exosomes. From our point of view, the most powerful clinicopathological factor predicting the distant metastasis site is a molecular subtype of primary tumor. We can conclude that luminal (HR+/HER2-) tumors are often characterized by single metastases and bones are the most common metastatic site, while TNBC and HER2-enriched tumors often metastasize to multiple sites, most commonly brain and liver. However, several authors did not reveal these associations in their studies. It likely indicates the existence of other factors that significantly affect the organotropism of metastasis. Numerous studies demonstrate the association of different molecules expressed on tumor cells with organotropic metastasis. However, these data are very fragmentary and rather contradictory.Conclusion. The found associations are common to all participants of metastatic cascade, but remains unclear which factors are essential and crucial in determining the direction of metastasis.ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅. ΠΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π² ΠΎΡΠ΄Π°Π»Π΅Π½Π½ΡΠ΅ ΠΎΡΠ³Π°Π½Ρ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΉ ΠΏΡΠΈΡΠΈΠ½ΠΎΠΉ ΡΠΌΠ΅ΡΡΠΈ ΠΏΡΠΈ ΡΠ°ΠΊΠ΅ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ. ΠΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²Π΅Π½Π½Π°Ρ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ ΠΎΡΠ΄Π°Π»Π΅Π½Π½ΡΡ
ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΎΠ² ΠΏΡΠΈ ΡΠ°ΠΊΠ΅ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ β ΠΊΠΎΡΡΠΈ, Π»Π΅Π³ΠΊΠΈΠ΅ ΠΈ ΠΏΠ΅ΡΠ΅Π½Ρ. ΠΠΎΠ·Π½ΠΈΠΊΠ°Π΅Ρ Π²ΠΎΠΏΡΠΎΡ, ΠΊΠ°ΠΊΠΈΠ΅ ΡΠ°ΠΊΡΠΎΡΡ ΠΌΠΎΠ³ΡΡ Π²Π»ΠΈΡΡΡ Π½Π° Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΠ΅ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½ΠΈΡ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ Π² ΡΠΎΡ ΠΈΠ»ΠΈ ΠΈΠ½ΠΎΠΉ ΠΎΡΠ³Π°Π½.ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡ ΠΎΠ±ΠΎΠ±ΡΠΈΠ»ΠΈ ΠΈΠΌΠ΅ΡΡΠΈΠ΅ΡΡ Π² ΠΌΠΈΡΠΎΠ²ΠΎΠΉ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΠΎ ΠΌΠ΅ΡΠΎΠ΄Π°Ρ
ΠΏΡΠΎΠ³Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΎΡΠ΄Π°Π»Π΅Π½Π½ΡΡ
ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΎΠ² Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
ΡΠ°ΠΊΠΎΠΌ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π€Π°ΠΊΡΠΎΡΡ, ΡΠ²ΡΠ·Π°Π½Π½ΡΠ΅ Ρ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠ΅ΠΉ ΠΎΡΠ΄Π°Π»Π΅Π½Π½ΡΡ
ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΎΠ², ΠΌΡ ΡΠ°Π·Π΄Π΅Π»ΠΈΠ»ΠΈ Π½Π° Π΄Π²Π΅ ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ Π³ΡΡΠΏΠΏΡ: ΠΊΠ»ΠΈΠ½ΠΈΠΊΠΎ-ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ Π±ΠΎΠ»ΡΠ½ΡΡ
ΡΠ°ΠΊΠΎΠΌ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΠΈ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠ΅ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΠΌΠΈΠΊΡΠΎΠΎΠΊΡΡΠΆΠ΅Π½ΠΈΡ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
Β ΠΊΠ»Π΅ΡΠΎΠΊ (ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΡΡ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΈ ΡΠΈΡΠΊΡΠ»ΠΈΡΡΡΡΠΈΡ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ) ΠΈΠ»ΠΈ ΠΈΡ
ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ
β ΡΠΊΠ·ΠΎΡΠΎΠΌ. ΠΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅ΡΡΡ, ΡΡΠΎ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠΉ ΠΏΠΎΠ΄ΡΠΈΠΏ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠΉ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌ ΠΏΡΠ΅Π΄ΠΈΠΊΡΠΎΡΠΎΠΌ, ΠΏΡΠ΅Π΄ΡΠΊΠ°Π·ΡΠ²Π°ΡΡΠΈΠΌ ΠΌΠ΅ΡΡΠΎ ΠΎΡΠ΄Π°Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ. ΠΡΠΌΠΈΠ½Π°Π»ΡΠ½ΡΠ΅ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ (HR+/ HER2-) ΡΠ°ΡΠ΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΡΡΡΡ Π΅Π΄ΠΈΠ½ΠΈΡΠ½ΡΠΌΠΈ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·Π°ΠΌΠΈ, Ρ ΠΏΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΠ΅ΠΉ Π² ΠΊΠΎΡΡΡΡ
, Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ ΡΡΠΈΠΆΠ΄Ρ Π½Π΅Π³Π°ΡΠΈΠ²Π½ΡΠ΅ ΠΈ HER2-ΠΎΠ±ΠΎΠ³Π°ΡΠ΅Π½Π½ΡΠ΅ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΡΠ°ΡΠ΅ ΠΎΠ±ΡΠ°Π·ΡΡΡ ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²Π΅Π½Π½ΡΠ΅ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·Ρ, ΠΊΠΎΡΠΎΡΡΠ΅ ΡΠ°ΡΠ΅ Π»ΠΎΠΊΠ°Π»ΠΈΠ·ΡΡΡΡΡ Π² Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠΌ ΠΌΠΎΠ·Π³Π΅ ΠΈ ΠΏΠ΅ΡΠ΅Π½ΠΈ. ΠΠ΄Π½Π°ΠΊΠΎ Π½Π΅ΠΊΠΎΡΠΎΡΡΠ΅ Π°Π²ΡΠΎΡΡ Π½Π΅ ΠΎΠ±Π½Π°ΡΡΠΆΠΈΠ²Π°ΡΡ Π² ΡΠ²ΠΎΠΈΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡΡ
ΡΠ°ΠΊΠΈΡ
Π·Π°ΠΊΠΎΠ½ΠΎΠΌΠ΅ΡΠ½ΠΎΡΡΠ΅ΠΉ, ΡΡΠΎ, Π²Π΅ΡΠΎΡΡΠ½ΠΎ, ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΠ΅Ρ ΠΎ Π½Π°Π»ΠΈΡΠΈΠΈ Π΄ΡΡΠ³ΠΈΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ², ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ Π²Π»ΠΈΡΡΡΠΈΡ
Π½Π° ΠΎΡΠ³Π°Π½ΠΎΡΡΠΎΠΏΠ½ΠΎΡΡΡ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ. ΠΠ½ΠΎΠ³ΠΎΡΠΈΡΠ»Π΅Π½Π½ΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΡΡΡ Π°ΡΡΠΎΡΠΈΠ°ΡΠΈΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΌΠΎΠ»Π΅ΠΊΡΠ», ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΡΠ΅ΠΌΡΡ
Π½Π° ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΊΠ°Ρ
, Ρ ΠΎΡΠ³Π°Π½ΠΎΡΡΠΎΠΏΠ½ΡΠΌ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ. ΠΠ΄Π½Π°ΠΊΠΎ ΡΡΠΈ Π΄Π°Π½Π½ΡΠ΅ Π²Π΅ΡΡΠΌΠ° ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠ°ΡΠ½Ρ ΠΈ ΠΈΠ½ΠΎΠ³Π΄Π° Π²Π΅ΡΡΠΌΠ° ΠΏΡΠΎΡΠΈΠ²ΠΎΡΠ΅ΡΠΈΠ²Ρ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠ±Π½Π°ΡΡΠΆΠ΅Π½Π½ΡΠ΅ Π°ΡΡΠΎΡΠΈΠ°ΡΠΈΠΈ ΠΎΡΠ½ΠΎΡΡΡΡΡ ΠΊΠΎ Π²ΡΠ΅ΠΌ ΡΡΠ°ΡΡΠ½ΠΈΠΊΠ°ΠΌ ΠΌΠ΅ΡΠ°ΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΠ°ΡΠΊΠ°Π΄Π°, Π½ΠΎ ΠΎΡΡΠ°Π΅ΡΡΡ Π½Π΅ΡΡΠ½ΡΠΌ, ΠΊΠ°ΠΊΠΈΠ΅ ΡΠ°ΠΊΡΠΎΡΡ ΡΠ²Π»ΡΡΡΡΡ ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΌΠΈ ΠΈ ΡΠ΅ΡΠ°ΡΡΠΈΠΌΠΈ Π² ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠΈ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ
ΠΠΠ ΠΠ‘ ΠΠΠΠΠΠΠΠΠΠ§ ΠΠ«Π Π―ΠΠΠ (1940β2002) β ΠΠΠΠΠ«Π Π ΠΠ‘Π‘ΠΠΠ‘ΠΠΠ ΠΠΠΠΠΠΠ
The article presents a biographical sketch of B.N. Zyryanov, a prominent Russian oncologist, Director of Tomsk Cancer Research Institute in the period from 1987 to 2002, Academician of the Russian Academy of Medical Sciences, Professor.ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ Π±ΠΈΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΎΡΠ΅ΡΠΊ, ΠΏΠΎΡΠ²ΡΡΠ΅Π½Π½ΡΠΉ Π²ΠΈΠ΄Π½ΠΎΠΌΡ ΡΠΎΡΡΠΈΠΉΡΠΊΠΎΠΌΡ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³Ρ, Π΄ΠΈΡΠ΅ΠΊΡΠΎΡΡ Π’ΠΎΠΌΡΠΊΠΎΠ³ΠΎ ΠΠΠ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΠΈ Π² ΠΏΠ΅ΡΠΈΠΎΠ΄ Ρ 1987 ΠΏΠΎ 2002 Π³., Π°ΠΊΠ°Π΄Π΅ΠΌΠΈΠΊΡ Π ΠΠΠ, ΠΏΡΠΎΡΠ΅ΡΡΠΎΡΡ Π.Π. ΠΡΡΡΠ½ΠΎΠ²Ρ
Calcium phosphate coatings produced by radiofrequency magnetron sputtering method
Calcium phosphate coatings on titanium implants surface, produced by radio frequency (RF) magnetron sputtering method with hydroxyapatite solid target were investigated. It was found that produced coatings are calcium deficient compared to stoichiometric hydroxyapatite. The surface of the coatings is highly rough at the nanoscale and highly elastic. In vivo experiments on rats revealed that titanium implants with the calcium phosphate coatings do not cause negative tissue reaction after 6 months incubation period
Concurrent thermochemoradiotherapy for brain high-grade glioma
Despite the achievements in the current strategies for treatment, the prognosis in malignant glioma patients remains unsatisfactory. Hyperthermia is currently considered to be the most effective and universal modifier of radiotherapy and chemotherapy. Preliminary treatment outcomes for 28 patients with newly diagnosed (23) and recurrent (5) high-grade gliomas were presented. All the patients received multimodality treatment including surgery, thermoche-moradiotherapy followed by 4 cycles of adjuvant chemotherapy. All the patients endured thermochemoradiotherapy well. A complication, limited skin burn (II stage), was diagnosed in two cases and treated conservatively without treatment interruption. A month after thermochemoradiotherapy the results were as follows: complete regression was achieved in 4 cases, partial regression in 4 cases, stable disease in 14 cases and disease progression in 6 cases (one of them is pseudo-progression). After completing the adjuvant chemotherapy 2 more patients demonstrated complete response and 1 patient had disease progression. Introduction of local hyperthermia in multimodal therapy of malignant glioma does not impair the combined modality treatment tolerability of patients with malignant gliomas. A small number of studied patients and short follow-up time do not allow making reliable conclusions about the impact of local hyperthermia on the treatment outcomes; however, there is a tendency towards the increase in disease-free survival in the patients with newly diagnosed malignant gliomas
Physical therapy methods in the treatment and rehabilitation of cancer patients
The results of the effective use of magnetic laser therapy in the treatment and rehabilitation of cancer patients were presented. The effect of magnetic-laser therapy in the treatment of radiation-induced reactions in the patients with head and neck cancer and in the patients with breast cancer was analyzed. High efficiency of lymphedema and lymphorrhea treatment in the postoperative period in the patients with breast cancer was proved. The results of rehabilitation of the patients with gastric cancer after surgical treatment were presented. These data indicate a high effectiveness of different physical methods of treatment and rehabilitation of cancer patients
Numerical Simulation of the Medical Linear Accelerator Electron Beams Absorption by ABS-Plastic doped with Metal
In this paper the numerical simulation results of the dose spatial distribution of the medical electron beams in ABS-plastic doped with different concentrations of lead and zinc are shown. The dependences of the test material density on the lead and zinc mass concentrations are illustrated. The depth dose distributions of the medical electron beams in the modified ABS-plastic for three energies 6 MeV, 12 MeV and 20 MeV are tested. The electron beam shapes in the transverse plane in ABS-plastic doped with different concentrations of lead and zinc are presented
Development of personalized approach to the reconstruction of bone tissue defects using porous ceramic osteoimplants
A fundamentally new complex technique for preoperative planning and creation of individual implants from ceramics for the reconstruction of the maxillofacial region is clinically applicable and in demand in modern trends of reconstructive surgery were developed and approved. The developed method implies using of alumina based ceramic material, which meets the requirements for medical materials used in reconstructive surgery. It was shown that by varying the initial composition of the ceramic powder material, it is possible to achieve structural and mechanical affinity with the surrounding bone tissue. Building a 3D model of an osteoimplant on the basis of a computed tomography of the patient avoids the need for additional resection of the bone tissue and ensures complete geometric conformity of the osteoimplant to the prosthetic area. The method was approved by the reconstruction of the patient's skull-orbital complex using a three-dimensional personalized ceramic implant. The complete absence of the inflammatory reaction after surgery was observed
Dynamics of LINE-1 retrotransposon methylation levels in circulating DNA from lung cancer patients undergoing antitumor therapy
Malignant cell transformation is accompanied with abnormal DNA methylation, such as the hypermethylation of certain gene promoters and hypomethylation of retrotransposons. In particular, the hypomethylation of the human-specific family of LINE-1 retrotransposons was observed in lung cancer tissues. It is also known that the circulating DNA (cirDNA) of blood plasma and cell-surface-bound circulating DNA (csb-cirDNA) of cancer patients accumulate tumor-specific aberrantly methylated DNA fragments, which are currently considered to be valuable cancer markers. This work compares LINE-1 retrotransposon methylation patterns in cirDNA of 16 lung cancer patients before and after treatment. CirDNA was isolated from blood plasma, and csb-cirDNA fractions were obtained by successive elution with EDTA-containing phosphate buffered saline and trypsin. Concentrations of methylated LINE-1 region 1 copies (LINE-1-met) were assayed by real-time methylation-specific PCR. LINE-1 methylation levels were normalized to the concentration of LINE-1 region 2, which was independent of the methylation status (LINE-1-Ind). The concentrations of LINE-1-met and LINE-1-Ind in csb-cirDNA of lung cancer patients exhibited correlations before treatment (r = 0.54), after chemotherapy (r = 0.72), and after surgery (r = 0.83) (P 0.05, respectively). These results suggest a need for the further investigation of dynamic changes in levels of LINE-1 methylation depending on the antitumor therapy
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