26 research outputs found
Carcass Compound Materials Base on Fluoropolymer for Tissue Engineering in Orthopedics
In this work new type of composite materials for application as coatings for intramedullary implants in the field of orthopaedics and traumatology is offered. Method is based on ability of fluoropolymers to act as biologically inert binding agent and ability of fine-dyspersated hydroxyapatite powders to act as biologically active filling agent providing osteoinduction and osteoconduction processes. Results of investigations of adhesion, elastic and morphometric characteristics of offered composite were presented; chemical composition was determined. Estimation of toxicological properties, locally irritant action and hemolytic activity of offered composites was done according to GOST R ISO 10993. In vivo tests were carried out; it was shown that offered composites didnβt cause any negative tissue reactions and stimulated osteogenesis processes in ectopic bone formation test. Key words: Carcass Compound Materials; fluoropolymers; orthopaedi
Modification of the zirconia ceramics by different calcium phosphate coatings:comparative study
The aim of this study was to characterize different calcium phosphate
coatings and evaluate in vitro cell response of these materials to ceramics
implants. The physical and chemical properties of calcium phosphate coatings
formed by RF-magnetron sputtering of calcium phosphate tribasic,
hydroxyapatite, calcium phosphate monobasic, calcium phosphate dibasic
dihydrate and calcium pyrophosphate powders were characterized. Cell adhesion
and cell viability were examined on calcium phosphate coatings using
mesenchymal stem cells. The results of cytotoxicity measurements of the calcium
phosphate coatings revealed that only the coating obtained by RF-magnetron
sputtering of the calcium phosphate dibasic dihydrate and calcium phosphate
tribasic powders possessed lower cell viability than the zirconia substrate.
The coating formed by sputtering of the calcium phosphate tribasic powder
demonstrated more cells adhered onto its surface compared with other calcium
phosphate coatings
Fabrication and properties of L-arginine-doped PCL electrospun composite scaffolds
The article describes fabrication and properties of composite fibrous
scaffolds obtained by electrospinning of the solution of
poly({\epsilon}-caprolactone) and arginine in common solvent. The influence of
arginine content on structure, mechanical, surface and biological properties of
the scaffolds was investigated. It was found that with an increase of arginine
concentration diameter of the scaffold fibers was reduced, which was
accompanied by an increase of scaffold strength and Young modulus. It was
demonstrated that porosity and water contact angle of the scaffold are
independent from arginine content. The best cell adhesion and viability was
shown on scaffolds with arginine concentration from 0.5 to 1 % wt
Calcium phosphate coatings produced by radiofrequency magnetron sputtering method
Calcium phosphate coatings on titanium implants surface, produced by radio frequency (RF) magnetron sputtering method with hydroxyapatite solid target were investigated. It was found that produced coatings are calcium deficient compared to stoichiometric hydroxyapatite. The surface of the coatings is highly rough at the nanoscale and highly elastic. In vivo experiments on rats revealed that titanium implants with the calcium phosphate coatings do not cause negative tissue reaction after 6 months incubation period
The Fabrication of Bioresorbable Implants for Bone Defects Replacement Using Computer Tomogram and 3D Printing
The present work demonstrates the possibility of production of personalized implants from bioresorbable polymers designed for replacement of bone defects. The stages of creating a personalized implant are described, which include the obtaining of 3D model from a computer tomogram, development of the model with respect to shape of bone fitment bore using Autodesk Meshmixer software, and 3D printing process from bioresorbable polymers. The results of bioresorbable polymer scaffolds implantation in pre-clinical tests on laboratory animals are shown. The biological properties of new bioresorbable polymers based on poly(lactic acid) were studied during their subcutaneous, intramuscular, bone and intraosseous implantation in laboratory animals. In all cases, there was a lack of a fibrous capsule formation around the bioresorbable polymer over time. Also, during the performed study, conclusions were made on osteogenesis intensity depending on the initial state of bone tissue
Modification of the zirconia ceramics by different calcium phosphate coatings:comparative study
The aim of this study was to characterize different calcium phosphate
coatings and evaluate in vitro cell response of these materials to ceramics
implants. The physical and chemical properties of calcium phosphate coatings
formed by RF-magnetron sputtering of calcium phosphate tribasic,
hydroxyapatite, calcium phosphate monobasic, calcium phosphate dibasic
dihydrate and calcium pyrophosphate powders were characterized. Cell adhesion
and cell viability were examined on calcium phosphate coatings using
mesenchymal stem cells. The results of cytotoxicity measurements of the calcium
phosphate coatings revealed that only the coating obtained by RF-magnetron
sputtering of the calcium phosphate dibasic dihydrate and calcium phosphate
tribasic powders possessed lower cell viability than the zirconia substrate.
The coating formed by sputtering of the calcium phosphate tribasic powder
demonstrated more cells adhered onto its surface compared with other calcium
phosphate coatings
Composite implants coated with biodegradable polymers prevent stimulating tumor progression
In this experiment we studied oncologic safety of model implants created using the solution blow spinning method with the use of the PURASORB PL-38 polylactic acid polymer and organic mineral filler which was obtained via laser ablation of a solid target made of dibasic calcium phosphate dihydrate. For this purpose the implant was introduced into the area of Wistar ratsβ iliums, and on day 17 after the surgery the Walker sarcoma was transplanted into the area of the implant. We evaluated the implantβs influence on the primary tumor growth, hematogenous and lymphogenous metastasis of the Walker sarcoma. In comparison with sham operated animals the implant group demonstrated significant inhibition of hematogenous metastasis on day 34 after the surgery. The metastasis inhibition index (MII) equaled 94% and the metastases growth inhibition index (MGII) equaled 83%. The metastasis frequency of the Walker sarcoma in para aortic lymph nodes in the implant group was not statistically different from the control frequency; there was also no influence of the implant on the primary tumor growth noted. In case of the Walker sarcoma transplantation into the calf and the palmar pad of the ipsilateral limb to the one with the implant in the ilium, we could not note any attraction of tumor cells to the implant area, i.e. stimulation of the Walker sarcoma relapse by the implant. Thus, the research concluded that the studied implant meets the requirements of oncologic safety