Pressure-control purge panel for automatic butt welding

Modification of a purge panel for use in an automatic butt weld reduces the drop in pressure between the regulators and the weld head and tube purge fitting. The invention affects air regulators for plants, regulating circuits for pneumatic valves, and automatic welding machines

The Impact of Demographic Change on Intergenerational Transfers via Bequests

Transfers in the form of bequests have important implications for the intergenerational transmission of inequality. Demographic change has relevant consequences for the timing and size of bequests. For example, longer life implies that people receive bequests when they are older. Conversely, increasing generational length reduces the average age at which people are given bequests. We analyze the consequences of demographic change in the United States for the timing over the life course when individuals receive an inheritance and for the size of bequests. We evaluate trends in life expectancy at the mean age at childbearing as a proxy for timing at receipt of bequests. We complement formal demographic analysis with empirical estimates from the Panel Study of Income Dynamics (PSID) inheritance data for 1987-2010. We find that the long-term trend of increasing age at receipt of bequests and of increasing size of per-capita bequests received might have stalled, mainly because of changes in the timing of fertility. In the long term, the upward trend in age at which people receive bequests may resume as the expected linear gains in life expectancy would more than counteract recent increases in the mean age at childbearing. We showed that demographic change affects the size of bequests and the timing over the life course at which people receive them. As the need for economic resources varies over the life cycle, changes in the timing at receipt of bequests may have a differential impact on wealth inequality and affect patterns of multigenerational transfers of resources

Exact results for Casimir interactions between dielectric bodies: The weak-coupling or van der Waals Limit

In earlier papers we have applied multiple scattering techniques to calculate Casimir forces due to scalar fields between different bodies described by delta function potentials. When the coupling to the potentials became weak, closed-form results were obtained. We simplify this weak-coupling technique and apply it to the case of tenuous dielectric bodies, in which case the method involves the summation of van der Waals (Casimir-Polder) interactions. Once again exact results for finite bodies can be obtained. We present closed formulas describing the interaction between spheres and between cylinders, and between an infinite plate and a retangular slab of finite size. For such a slab, we consider the torque acting on it, and find non-trivial equilibrium points can occur.Comment: 4 pages, 3 figure

Gene transfer into hepatocytes using asialoglycoprotein receptor mediated endocytosis of DNA complexed with an artificial tetra-antennary galactose ligand

We have constructed an artificial ligand for the hepatocyte-specific asialoglycoprotein receptor for the purpose of generating a synthetic delivery system for DNA. This ligand has a tetra-antennary structure, containing four terminal galactose residues on a branched carrier peptide. The carbohydrate residues of this glycopeptide were introduced by reductive coupling of lactose to the alpha- and epsilon-amino groups of the two N-terminal lysines on the carrier peptide. The C-terminus of the peptide, containing a cysteine separated from the branched N-terminus by a 10 amino acid spacer sequence, was used for conjugation to 3-(2-pyridyldithio)propionate-modified polylysine via disulfide bond formation. Complexes containing plasmid DNA bound to these galactose-polylysine conjugates have been used for asialoglycoprotein receptor-mediated transfer of a luciferase gene into human (HepG2) and murine (BNL CL.2) hepatocyte cell lines. Gene transfer was strongly promoted when amphipathic peptides with pH-controlled membrane-disruption activity, derived from the N-terminal sequence of influenza virus hemagglutinin HA-2, were also present in these DNA complexes. Thus, we have essentially borrowed the small functional domains of two large proteins, asialoglycoprotein and hemagglutinin, and assembled them into a supramolecular complex to generate an efficient gene-transfer system

About the determination of critical exponents related to possible phase transitions in nuclear fragmentation

We introduce a method based on the finite size scaling assumption which allows to determine numerically the critical point and critical exponents related to observables in an infinite system starting from the knowledge of the observables in finite systems. We apply the method to bond percolation in 2 dimensions and compare the results obtained when the bond probability p or the fragment multiplicity m are chosen as the relevant parameter.Comment: 12 pages, TeX, 4 figure

Defect free global minima in Thomson's problem of charges on a sphere

Given $N$ unit points charges on the surface of a unit conducting sphere, what configuration of charges minimizes the Coulombic energy $\sum_{i>j=1}^N 1/r_{ij}$? Due to an exponential rise in good local minima, finding global minima for this problem, or even approaches to do so has proven extremely difficult. For \hbox{$N = 10(h^2+hk+k^2)+ 2$} recent theoretical work based on elasticity theory, and subsequent numerical work has shown, that for $N \sim >500$--1000 adding dislocation defects to a symmetric icosadeltahedral lattice lowers the energy. Here we show that in fact this approach holds for all $N$, and we give a complete or near complete catalogue of defect free global minima.Comment: Revisions in Tables and Reference

Acid-Labile Traceless Click Linker for Protein Transduction

Intracellular delivery of active proteins presents an interesting approach in research and therapy. We created a protein transduction shuttle based on a new traceless click linker that combines the advantages of click reactions with implementation of reversible pH-sensitive bonds. The azidomethyl-methylmaleic anhydride (AzMMMan) linker was found compatible with different click chemistries, demonstrated in bioreversible protein modification with dyes, polyethylene glycol, or a transduction carrier. Linkages were stable at physiological pH but reversible at the mild acidic pH of endosomes or lysosomes. We show that pH-reversible attachment of a defined endosome-destabilizing three-arm oligo(ethane amino)amide carrier generates an effective shuttle for protein delivery. The cargo protein nlsEGFP, when coupled via the traceless AzMMMan linker, experiences efficient cellular uptake and endosomal escape into the cytosol, followed by import into the nucleus. In contrast, irreversible linkage to the same shuttle hampers nuclear delivery of nlsEGFP which after uptake remains trapped in the cytosol. Successful intracellular delivery of bioactive ß-galactosidase as a model enzyme was also demonstrated using the pH-controlled shuttle system

Implications of sterile neutrinos for medium/long-baseline neutrino experiments and the determination of θ13\theta_{13}

We revisit some of the recent neutrino observations and anomalies in the context of sterile neutrinos. Based on a general parametrization motivated in the presence of sterile neutrinos, the consistency of the MINOS disappearance data with additional sterile neutrinos is discussed. We also explore the implications of sterile neutrinos for the measurement of $|U_{\mu3}|$ in this case. Regarding the determination of $|U_{e3}|$, we observe that the existence of sterile neutrinos may induce a significant modification of the $\theta_{13}$ angle in neutrino appearance experiments like T2K and MINOS, over and above the ambiguities and degeneracies that are already present in 3-neutrino parameter extractions. The modification is less significant in reactor neutrino experiments like Double-CHOOZ, Daya Bay and RENO and therefore the extracted $|U_{e3}|$ value when sterile neutrinos are present is close to the one that would be obtained in the 3-neutrino case. We also conclude that the results from T2K imply a 90% C.L. lower-bound on $|U_{e3}|$, in the "$\,3+2$" neutrino case, which is still within the sensitivity of future reactor neutrino experiments like Daya Bay, and consistent with the one-$\sigma$ range of $\sin^22\theta_{13}$ recently reported by the Double-CHOOZ experiment. Finally, we argue that for the recently determined best-fit parameters, the results in the "$\,3+1$" scenario would be very close to the medium/long baseline results obtained in the "$\,3+2$" case analyzed in this work.Comment: 25 pages, 9 figures, revtex4-1. Typos corrected, published versio

Delay of Disorder by Diluted Polymers

We study the effect of diluted flexible polymers on a disordered capillary wave state. The waves are generated at an interface of a dyed water sugar solution and a low viscous silicon oil. This allows for a quantitative measurement of the spatio-temporal Fourier spectrum. The primary pattern after the first bifurcation from the flat interface are squares. With increasing driving strength we observe a melting of the square pattern. It is replaced by a weak turbulent cascade. The addition of a small amount of polymers to the water layer does not affect the critical acceleration but shifts the disorder transition to higher driving strenghs and the short wave length - high frequency fluctuations are suppressed