Small regulatory RNAs in vascular remodeling and atherosclerosis

Cardiovascular disease (CVD) is a major cause of death worldwide. The underlying cause of most CVD is atherosclerosis. Atherosclerosis is characterized by progressive plaque build-up in the arterial wall.Noncoding RNAs (ncRNAs) are RNAs that are not translated into protein. This thesis focuses on two types: microRNAs and small nucleolar RNAs (snoRNAs). MicroRNAs inhibit the production of proteins and act on multiple proteins simultaneously. In CVD, many different proteins are involved. Changing expression of one microRNA can therefore have a major impact.Numerous snoRNAs have been associated with diseases, including CVD. The function of half of the human C/D box snoRNAs, however, is unknown.The first aim of this thesis is to investigate inhibition of microRNA-494-3p in advanced atherosclerosis. The second aim is to elucidate the function of SNORD113-6, a snoRNA that is involved in CVD.The thesis shows that inhibition of microRNA-494-3p halts plaque progression and increases stability of advanced plaques. This reduces the risk of e.g. a myocardial infarction.Furthermore, SNORD113-6 influences the function of fibroblasts, scar cells, and thus plays a role in maintaining function of our blood vessels.These insights may open up new therapeutic possibilities in future treatment of CVD.The research described in this thesis was supported by a grant from the Rembrandt Institute of Cardiovascular Science (2016).LUMC / Geneeskund

Neighborhood stigma and the sporting lives of young people in public housing

This paper spotlights the sporting lives of young people who live in ‘Redcrest’, a public housing community in the Niagara region of Canada. We report on data culled from neighborhoodcentric documents (municipal data, planning council reports, media coverage) and ethnographic fieldwork (interviews, community mapping, go-alongs) collected over eight months with 14 young people. This paper also offers a critique of Robert Sampson’s work on neighborhood effects and draws on the theoretical insights and urban scholarship of Henri Lefebvre, Loic Wacquant, and the work of postcolonial scholar Frantz Fanon, who further understandings of racism as a spatial relation. At the center of this research are narratives that highlight that public housing projects, negative stigma notwithstanding, can be good places to live. The results highlight the various contradictions and tensions experienced by young people living in Redcrest, specifically their experiences with neighborhood stigma, racism and Islamophobia, and how this impacts their sporting lives

The Health-e-Waterways Project: Data Integration for Smarter Collaborative Whole-of-Water Cycle Management

The Health-e-Waterways Project is a collaboration between the University of Queensland, Microsoft Research and the South East Queensland Healthy Waterways Partnership (SEQ-HWP) (a consortium of over 60 local government, state agency, universities, community and environmental organizations). The aim of the project is to develop a highly innovative framework and set of services to enable streamlined access to a collection of real-time, near-real-time and static datasets acquired through ecosystem health monitoring programs (EHMP) in South East Queensland. This paper describes the underlying water information management system and Web Portal that we are developing to enable the sharing and integration of the high quality data and models for SEQ water resource managers. In addition we will describe the interactive and dynamic ecosystem reporting services that we have developed and the WaterWiki that is being established to enable knowledge exchange between the online community of Queensland’s water stakeholders

A Good Place for What? Placing ‘Value’ in Youth Centres

In this paper, we investigate the discursive context of community-based youth centres to critically interrogate ideas and practices concerning leisure, youth, and youth centres. Using publicly available documents and data collected with youth at two community-based youth centres, we ask, what is the “good”’ that they do for young people, and how do young people negotiate these discourses? We argue that the youth centres operate in a discursive tension, constructed as a place to change by the (organizational) bodies that established them, and a place to chill by the (youth) bodies that used them. We trace how these discourses entered into the everyday lived contexts of youth centres including their program logics, measures of success, and constructions of youthful subjectivities. We close with a discussion of the implications of the research in terms of how youth and recreation practitioners might use youth centres to support young people’s leisure

The orbit space of groupoids whose C∗C^*-algebras are GCR

Let $G$ be second countable locally compact Hausdorff groupoid with a continuous Haar system. We remove the assumption of amenability in a theorem by Clark about GCR groupoid $C^*$-algebras. We show that if the groupoid $C^*$-algebra of $G$ is GCR then the orbits of $G$ are locally closed.Comment: 1

No agreement of mixed venous and central venous saturation in sepsis, independent of sepsis origin

Introduction: Controversy remains regarding the relationship between central venous saturation (ScvO(2)) and mixed venous saturation (SvO(2)) and their use and interchangeability in patients with sepsis or septic shock. We tested the hypothesis that ScvO(2) does not reliably predict SvO(2) in sepsis. Additionally we looked at the influence of the source (splanchnic or non-splanchnic) of sepsis on this relationship. Methods: In this prospective observational two-center study we concurrently determined ScvO(2) and SvO(2) in a group of 53 patients with severe sepsis during the first 24 hours after admission to the intensive care units in 2 Dutch hospitals. We assessed correlation and agreement of ScvO(2) and SvO(2), including the difference, i.e. the gradient, between ScvO(2) and SvO(2) (ScvO(2) -SvO(2)). Additionally, we compared the mean differences between ScvO(2) and SvO(2) of both splanchnic and non-splanchnic group. Results: A total of 265 paired blood samples were obtained. ScvO(2) overestimated SvO(2) by less than 5% with wide limits of agreement. For changes in ScvO(2) and SvO(2) results were similar. The distribution of the (ScvO(2) - SvO(2)) (< 0 or >= 0) was similar in survivors and nonsurvivors. The mean (ScvO(2) - SvO(2)) in the splanchnic group was similar to the mean (ScvO(2) - SvO(2)) in the non-splanchnic group (0.8 +/- 3.9% vs. 2.5 +/- 6.2%; P = 0.30). O2ER (P = 0.23) and its predictive value for outcome (P = 0.20) were similar in both groups. Conclusions: ScvO(2) does not reliably predict SvO(2) in patients with severe sepsis. The trend of ScvO(2) is not superior to the absolute value in this context. A positive difference (ScvO(2) -SvO(2)) is not associated with improved outcome