4,292 research outputs found
Recommended from our members
The leber congenital amaurosis protein AIPL1 and EB proteins co-localize at the photoreceptor cilium
Purpose: The aim of this study was to investigate the interaction and co-localization of novel interacting proteins with the Leber congenital amaurosis (LCA) associated protein aryl hydrocarbon receptor interacting protein-like 1 (AIPL1).
Methods: The CytoTrapXR yeast two-hybrid system was used to screen a bovine retinal cDNA library. A novel interaction between AIPL1 and members of the family of EB proteins was confirmed by directed yeast two-hybrid analysis and co-immunoprecipitation assays. The localization of AIPL1 and the EB proteins in cultured cells and in retinal cryosections was examined by immunofluorescence microscopy and cryo-immunogold electron microscopy.
Results: Yeast two-hybrid (Y2H) analysis identified the interaction between AIPL1 and the EB proteins, EB1 and EB3. EB1 and EB3 were specifically co-immunoprecipitated with AIPL1 from SK-N-SH neuroblastoma cells. In directed 1:1 Y2H analysis, the interaction of EB1 with AIPL1 harbouring the LCA-causing mutations A197P, C239R and W278X was severely compromised. Immunofluorescent confocal microscopy revealed that AIPL1 did not co-localize with endogenous EB1 at the tips of microtubules, endogenous EB1 at the microtu-bule organising centre following disruption of the microtubule network, or with endogenous β-tubulin. Moreover, AIPL1 did not localize to primary cilia in ARPE-19 cells, whereas EB1 co-localized with the centrosomal marker pericentrin at the base of primary cilia. However, both AIPL1 and the EB proteins, EB1 and EB3, co-localized with centrin-3 in the connecting cilium of photoreceptor cells. Cryo-immunogold electron microscopy confirmed the co-localization of AIPL1 and EB1 in the connecting cilia in human retinal photoreceptors.
Conclusions: AIPL1 and the EB proteins, EB1 and EB3, localize at the connecting cilia of retinal photore-ceptor cells, but do not co-localize in the cellular microtubule network or in primary cilia in non-retinal cells. These findings suggest that AIPL1 function in these cells is not related to the role of EB proteins in microtubule dynamics or primary ciliogenesis, but that their association may be related to a specific role in the specialized cilia apparatus of retinal photoreceptors
Quantum dot dephasing by edge states
We calculate the dephasing rate of an electron state in a pinched quantum
dot, due to Coulomb interactions between the electron in the dot and electrons
in a nearby voltage biased ballistic nanostructure. The dephasing is caused by
nonequilibrium time fluctuations of the electron density in the nanostructure,
which create random electric fields in the dot. As a result, the electron level
in the dot fluctuates in time, and the coherent part of the resonant
transmission through the dot is suppressed
Thomas-Fermi-Dirac-von Weizsacker hydrodynamics in laterally modulated electronic systems
We have studied the collective plasma excitations of a two-dimensional
electron gas with an arbitrary lateral charge-density modulation. The dynamics
is formulated using a previously developed hydrodynamic theory based on the
Thomas-Fermi-Dirac-von Weizsacker approximation. In this approach, both the
equilibrium and dynamical properties of the periodically modulated electron gas
are treated in a consistent fashion. We pay particular attention to the
evolution of the collective excitations as the system undergoes the transition
from the ideal two-dimensional limit to the highly-localized one-dimensional
limit. We also calculate the power absorption in the long-wavelength limit to
illustrate the effect of the modulation on the modes probed by far-infrared
(FIR) transmission spectroscopy.Comment: 27 page Revtex file, 15 Postscript figure
Kohn Anomalies in Superconductors
I present the detailed behavior of phonon dispersion curves near momenta
which span the electronic Fermi sea in a superconductor. I demonstrate that an
anomaly, similar to the metallic Kohn anomaly, exists in a superconductor's
dispersion curves when the frequency of the phonon spanning the Fermi sea
exceeds twice the superconducting energy gap. This anomaly occurs at
approximately the same momentum but is {\it stronger} than the normal-state
Kohn anomaly. It also survives at finite temperature, unlike the metallic
anomaly. Determination of Fermi surface diameters from the location of these
anomalies, therefore, may be more successful in the superconducting phase than
in the normal state. However, the superconductor's anomaly fades rapidly with
increased phonon frequency and becomes unobservable when the phonon frequency
greatly exceeds the gap. This constraint makes these anomalies useful only in
high-temperature superconductors such as .Comment: 18 pages (revtex) + 11 figures (upon request), NSF-ITP-93-7
Spin orbit effects in a GaAs quantum dot in a parallel magnetic field
We analyze the effects of spin-orbit coupling on fluctuations of the
conductance of a quantum dot fabricated in a GaAs heterostructure. We argue
that spin-orbit effects may become important in the presence of a large
parallel magnetic field B_{||}, even if they are negligble for B_{||}=0. This
should be manifest in the level repulsion of a closed dot, and in reduced
conductance fluctuations in dots with a small number of open channels in each
lead, for large B_{||}. Our picture is consistent with the experimental
observations of Folk et al.Comment: 5 page
DiVinE-CUDA - A Tool for GPU Accelerated LTL Model Checking
In this paper we present a tool that performs CUDA accelerated LTL Model
Checking. The tool exploits parallel algorithm MAP adjusted to the NVIDIA CUDA
architecture in order to efficiently detect the presence of accepting cycles in
a directed graph. Accepting cycle detection is the core algorithmic procedure
in automata-based LTL Model Checking. We demonstrate that the tool outperforms
non-accelerated version of the algorithm and we discuss where the limits of the
tool are and what we intend to do in the future to avoid them
Dephasing of Electrons by Two-Level Defects in Quantum Dots
The electron dephasing time in a diffusive quantum dot is
calculated by considering the interaction between the electron and dynamical
defects, modelled as two-level system. Using the standard tunneling model of
glasses, we obtain a linear temperature dependence of ,
consistent with the experimental observation. However, we find that, in order
to obtain dephasing times on the order of nanoseconds, the number of two-level
defects needs to be substantially larger than the typical concentration in
glasses. We also find a finite system-size dependence of , which
can be used to probe the effectiveness of surface-aggregated defects.Comment: two-column 9 page
Dynamic exchange-correlation potentials for the electron gas in dimensionality D=3 and D=2
Recent progress in the formulation of a fully dynamical local approximation
to time-dependent Density Functional Theory appeals to the longitudinal and
transverse components of the exchange and correlation kernel in the linear
current-density response of the homogeneous fluid at long wavelength. Both
components are evaluated for the electron gas in dimensionality D=3 and D=2 by
an approximate decoupling in the equation of motion for the current density,
which accounts for processes of excitation of two electron-hole pairs. Each
pair is treated in the random phase approximation, but the role of exchange and
correlation is also examined; in addition, final-state exchange processes are
included phenomenologically so as to satisfy the exactly known high-frequency
behaviours of the kernel. The transverse and longitudinal spectra involve the
same decay channels and are similar in shape. A two-plasmon threshold in the
spectrum for two-pair excitations in D=3 leads to a sharp minimum in the real
part of the exchange and correlation kernel at twice the plasma frequency. In
D=2 the same mechanism leads to a broad spectral peak and to a broad minimum in
the real part of the kernel, as a consequence of the dispersion law of the
plasmon vanishing at long wavelength. The numerical results have been fitted to
simple analytic functions.Comment: 13 pages, 11 figures included. Accepted for publication in Phys. Rev.
Coulomb drag in compressible quantum Hall states
We consider the Coulomb drag between two layers of two-dimensional electronic
gases subject to a strong magnetic field. We first focus on the case in which
the electronic density is such that the Landau level filling fraction in
each layer is at, or close to, . Discussing the coupling between the
layers in purely electronic terms, we show that the unique dependence of the
longitudinal conductivity on wave-vector, observed in surface acoustic waves
experiments, leads to a very slow decay of density fluctuations. Consequently,
it has a crucial effect on the Coulomb drag, as manifested in the
transresistivity . We find that the transresistivity is very large
compared to its typical values at zero magnetic field, and that its temperature
dependence is unique -- . For filling factors at or
close to and the transresistivity has the same -dependence, and
is larger than at . We calculate for the case and
propose that it might shed light on the spin polarization of electrons at
. We compare our results to recent calculations of at
where a composite fermion approach was used and a
-dependence was obtained. We conclude that what appears in the
composite fermion language to be drag induced by Chern-Simons interaction is,
physically, electronic Coulomb drag.Comment: 11 pages, REVTeX with two Postscript figure
- …