23 research outputs found
Adaptação metodológica de citogenética pra minhocas.
O objetivo do presente trabalho foi testar diferentes tĂ©cnicas citogenĂ©ticas em minhocas da espĂ©cie Eisenia andrei BouchĂ©, no intuito de estabelecer uma diretriz para novos estudos que proporcionem a obtenção de material com maior nĂșmero de metĂĄfases com menos custo e tempo de desenvolvimento
Citogenética da espécie exótica de minhoca Eisenia andrei Bouché (oligochaeta, lumbricidae).
Study of four Neotropical species of tree crickets Oecanthus Serville, 1831 (Orthoptera, Gryllidae) using cytogenetic and molecular markers
Karyotypes in the worldwide subfamily Oecanthinae show variations in diploid number, chromosome morphology, and sex-chromosome system. This study described the chromosome set and phylogenetic relationships of four Neotropical species, Oecanthus lineolatus, O. valensis, O. pallidus, and O. pictus. We used classical cytogenetics and Bayesian Inference for phylogenetic reconstruction, using the mitochondrial genes COI, 12S rRNA, and 16S rRNA; and analyzed the phylogenetic patterns of changes in chromosome numbers, using ChromEvol. We observed differences in chromosome number among species and two different sex-chromosome systems. Oecanthus pictus showed 2n = 21, X0â/22, XXâ; O. lineolatus, 2n = 20, XYâ/XXâ; and O. valensis and O. pallidus, 2n = 18, XYâ/XXâ. The karyotype of Oecanthus was asymmetric, one group with large chromosomes and variation in heterochromatin distribution, and another with small acrocentric chromosomes. The phylogenetic tree recovered two main groups: one with the Palearctic species and another with species from different bioregions, but with low posterior probability. The Neotropical species grouped separately, O. valensis and O. pictus with Nearctic and Ethiopian species, and O. pallidus and O. lineolatus in another, well-supported clade. Together, the phylogenic and chromosome data suggest descending dysploidy events during the evolution of the group
Understanding the chromosomal evolution in cuckoos (Aves, Cuculiformes): a journey through unusual rearrangements
The Cuculiformes are a family of over 150 species that live in a range of habitats, such as forests, savannas, and deserts. Here, bacterial artificial chromosome (BAC) probes (75 from chicken and 14 from zebra finch macrochromosomes 1â10 +ZW and for microchromosomes 11â28 (except 16)) were used to investigate chromosome homologies between chicken and the squirrel cuckoo ( Piaya cayana). In addition, repetitive DNA probes were applied to characterize the chromosome organization and to explore the role of these sequences in the karyotype evolution of P. cayana. We also applied BAC probes for chicken chromosome 17 and Z to the guira cuckoo ( Guira guira) to test whether this species has an unusual Robertsonian translocation between a microchromosome and the Z chromosome, recently described in the smooth-billed ani ( Crotophaga ani). Our results revealed extensive chromosome reorganization with inter- and intrachromosomal rearrangements in P. cayana, including a conspicuous chromosome size and heterochromatin polymorphism on chromosome pair 20. Furthermore, we confirmed that the Z-autosome Robertsonian translocation found in C. ani is also found in G. guira, not P. cayana. These findings suggest that this translocation occurred prior to the divergence between C. ani and G. guira, but after the divergence with P. cayana
Stridulatory file and calling song of two populations of the tropical bush cricket Eneoptera surinamensis (Orthoptera, Gryllidae, Eneopterinae)
Autosomal rearrangement in Gryllus assimilis Fabricius, 1775 (Orthoptera, Gryllidae)
Gryllus assimilis L. has a karyotype of 2n = 29 (X0, male) and 30 (XX, female). The above karyotype was encountered along with another in which 2n = 28 (X0, male) and 2n = 29 (XX, female) in a population from the outskirts of Rio Claro city (SĂŁo Paulo State, Brazil). Of eight specimens studied, five had the heterozygous karyotype involving a translocation and three had the basic karyotype. There were no individuals homozygous for the rearrangement. The heterozygous karyotype was the result of a chromosomal rearrangement between chromosome pairs 6 and 10, both of which were initially submetacentric. The members of the sixth pair normally have two constrictions in the small arm, with a satellite at the chromosome tip. The chromosome of the tenth pair involved in the translocation was generally submetacentric and probably underwent a pericentric inversion which transported the centromere to a subterminal position before being translocated. In this case, the long arm of the inverted chromosome of the tenth pair was translocated with the satellite of a member of the sixth pair.<br>A espĂ©cie Gryllus assimilis L. apresenta 2n = 29, X0 (macho) e 2n = 30, XX (fĂȘmea), porĂ©m em alguns indivĂduos coletados na cidade de Rio Claro, SĂŁo Paulo, Brasil, foram encontrados dois cariĂłtipos distintos: o cariĂłtipo bĂĄsico e outro rearranjado, com 2n = 28, X0 e 2n = 29, XX. O rearranjo foi interpretado como sendo autossĂŽmico e heterozigoto, caracterizado pela translocação envolvendo dois pares de homĂłlogos submetacĂȘntricos: o par 10, que possivelmente tem um dos elementos com uma inversĂŁo pericĂȘntrica, e o par 6, que possui em seu braço curto duas constriçÔes secundĂĄrias, diferenciando satĂ©lites em suas extremidades