Gauge dependence of calculations in relativistic Coulomb excitation

Before a quantum-mechanical calculation involving electromagnetic interactions is performed, a choice must be made of the gauge to be used in expressing the potentials. If the calculation is done exactly, the observable results it predicts will be independent of the choice of gauge. However, in most practical calculations approximations are made, which can destroy the gauge invariance of the predictions. We compare here the results of coupled-channel time-dependent relativistic Coulomb excitation calculations, as performed in either Lorentz or Coulomb gauges. We find significant differences when the bombarding energy per nucleon is $\geq$ 2 GeV, which indicates that the common practice of relying completely on the Lorentz gauge can be dangerous.Comment: 23 pages, 3 figure

Coulomb Excitation of Multi-Phonon Levels of the Giant Dipole Resonance

A closed expression is obtained for the cross-section for Coulomb excitation of levels of the giant dipole resonance of given angular momentum and phonon number. Applications are made to the Goldhaber-Teller and Steinwedel-Jensen descriptions of the resonance, at non-relativistic and relativistic bombarding energies.Comment: 16 pages, 5 figure

A dataset of tracer concentrations and meteorological observations from the Bolzano Tracer EXperiment (BTEX) to characterize pollutant dispersion processes in an Alpine valley

Abstract. The paper describes the dataset of concentrations and related meteorological measurements collected during the field campaign of the Bolzano Tracer Experiment (BTEX). The experiment was performed to characterize the dispersion of pollutants emitted from a waste incinerator in the basin of the city of Bolzano, in the Italian Alps. As part of the experiment, two controlled releases of a passive gas tracer (sulfur hexafluoride, SF6) were performed through the stack of the incinerator on 14 February 2017 for two different time lags, starting, respectively, at 07:00 and 12:45 LST. Samples of ambient air were collected at target sites with vacuum-filled glass bottles and polyvinyl fluoride bags, and they were later analyzed by means of a mass spectrometer (detectability limit 30 pptv). Meteorological conditions were monitored by a network of 15 surface weather stations, 1 microwave temperature profiler, 1 sodar and 1 Doppler wind lidar. The dataset represents one of the few examples available in the literature concerning dispersion processes in a typical mountain valley environment, and it provides a useful benchmark for testing atmospheric dispersion models in complex terrain. The dataset described in this paper is available at https://doi.org/10.1594/PANGAEA.898761 (Falocchi et al., 2019)

A mechanistic investigation of the ruthenium porphyrin catalysed aziridination of olefins by aryl azides

A combination of DFT and kinetic studies suggests a mechanism for olefin aziridination by organic azides catalysed by ruthenium porphyrins

Hologene 5: A Phase II/III Clinical Trial of Combined Cell and Gene Therapy of Junctional Epidermolysis Bullosa

Epidermolysis bullosa (EB) is a group of devastating genetic diseases characterized by skin and mucosal fragility and formation of blisters, which develop either spontaneously or in response to minor mechanical trauma. There is no definitive therapy for any form of EB. Intermediate junctional EB (JEB) caused by mutations in the gene LAMB3 has been the first genetic skin disease successfully tackled by ex vivo gene therapy. Here, we present a multicenter, open-label, uncontrolled phase II/III study that aims at confirming the efficacy of Hologene 5, a graft consisting of cultured transgenic keratinocytes and epidermal stem cells and meant to combine cell and gene therapy for the treatment of LAMB3-related JEB. Autologous clonogenic keratinocytes will be isolated from patients’ skin biopsies, genetically corrected with a gamma-retroviral vector (γRV) carrying the full-length human LAMB3 cDNA and plated onto a fibrin support (144cm2). The transgenic epidermis will be transplanted onto surgically prepared selected skin areas of at least six JEB patients (four pediatric and two adults). Evaluation of clinical efficacy will include, as primary endpoint, a combination of clinical parameters, such as percentage of re-epithelialization, cellular, molecular, and functional parameters, mechanical stress tests, and patient-reported outcome (PRO), up to 12months after transplantation. Safety and further efficacy endpoints will also be assessed during the clinical trial and for additional 15years in an interventional non-pharmacological follow-up study. If successful, this clinical trial would provide a therapeutic option for skin lesions of JEB patients with LAMB3 mutations and pave the way to a combined cell and gene therapy platform tackling other forms of EB and different genodermatoses. Clinical Trial Registration: EudraCT Number: 2018-000261-36

Organic azides : energetic reagents for the intermolecular amination of C-H bonds

This feature article provides an overview of the application of organic azides for the intermolecular amination of sp(3) and sp(2) C-H bonds. The catalytic activity of several metal complexes was reviewed underlining both synthetic and mechanistic aspects of the C-H amination. The majority of the aminated compounds reported in literature have been collected in this paper to provide a compendium of published procedures. In addition, the discussion of involved mechanisms has been included to assist the reader to envisage the future potential of organic azides in the synthesis of aza-derivatives

Superior mesenteric artery syndrome diagnosed with linear endoscopic ultrasound

n/

Synthesis and catalytic activity of μ-​oxo ruthenium(IV) porphyrin species to promote amination reactions

This work describes the synthesis of ruthenium(IV) mu-oxo porphyrin complexes of general formula [Ru-IV(TPP)(X)](2)O which have been applied as catalysts in nitrene transfer reactions using aryl azides (ArN3) as nitrene sources. Collected data indicated that the catalytic efficiency of [Ru-IV(TPP)(OCH3)](2)O was comparable to that of Ru-II(TPP)CO because of their analogous reactivity towards aryl azides to give the same catalytically active bis-imido species Ru-VI(TPP)(ArN)(2). The reaction of [RuIV(TPP)(OCH3)](2)O with Ph3CN3 or (CH3)(3)SiN3 afforded [RuIV(TPP)(N-3)](2)O which was fully characterised, its molecular structure was also determined by single crystal X-ray analysis

Effort angina in a patient with advanced coronary artery disease. Role played by coronary angiography, Ivus and cardiac CT: case report

Coronary angiography is considered to be the gold standard technique for assessing the severity of obstructive luminal narrowing; however, in a few circumstances it may be misleading. In these cases, cardiac computed tomography (CT) and intravascular ultrasound (IVUS) may help to give a correct interpretation

Characterization of ten highly polymorphic microsatellite loci for the intertidal mussel Perna perna, and cross species amplification within the genus

The brown mussel Perna perna (Linnaeus, 1758) is a dominant constituent of intertidal communities and a strong invader with multiple non-native populations distributed around the world. In a previous study, two polymorphic microsatellite loci were developed and used to determine population-level genetic diversity in invasive and native P. perna populations. However, higher number of microsatellite markers are required for reliable population genetic studies. In this context, in order to understand P. perna origins and history of invasion and to compare population genetic structure in native versus invaded areas, we developed 10 polymorphic microsatellite markers. Findings Described microsatellite markers were developed from an enriched genomic library. Analyses and characterization of loci using 20 individuals from a population in Western Sahara revealed on average 11 alleles per locus (range: 5–27) and mean gene diversity of 0.75 (range: 0.31 - 0.95). One primer pair revealed possible linkage disequilibrium while heterozygote deficiency was significant at four loci. Six of these markers cross-amplified in P. canaliculus (origin: New Zealand). Conclusions Developed markers will be useful in addressing a variety of questions concerning P. perna, including dispersal scales, genetic variation and population structure, in both native and invaded areas.Peer Reviewe