Sunyaev-Zel'dovich clusters reconstruction in multiband bolometer camera surveys

We present a new method for the reconstruction of Sunyaev-Zel'dovich (SZ) galaxy clusters in future SZ-survey experiments using multiband bolometer cameras such as Olimpo, APEX, or Planck. Our goal is to optimise SZ-Cluster extraction from our observed noisy maps. We wish to emphasize that none of the algorithms used in the detection chain is tuned on prior knowledge on the SZ -Cluster signal, or other astrophysical sources (Optical Spectrum, Noise Covariance Matrix, or covariance of SZ Cluster wavelet coefficients). First, a blind separation of the different astrophysical components which contribute to the observations is conducted using an Independent Component Analysis (ICA) method. Then, a recent non linear filtering technique in the wavelet domain, based on multiscale entropy and the False Discovery Rate (FDR) method, is used to detect and reconstruct the galaxy clusters. Finally, we use the Source Extractor software to identify the detected clusters. The proposed method was applied on realistic simulations of observations. As for global detection efficiency, this new method is impressive as it provides comparable results to Pierpaoli et al. method being however a blind algorithm. Preprint with full resolution figures is available at the URL: w10-dapnia.saclay.cea.fr/Phocea/Vie_des_labos/Ast/ast_visu.php?id_ast=728Comment: Submitted to A&A. 32 Pages, text onl

Propagation of Casuarina equisetifolia through axillary buds of immature female inflorescences cultured in vitro

The study of the actinorhizal symbiosis in #Casuarina equisetifolia$ requires an homogenous plant material. Consequently, we devised a method of micropropagation based on the use of immature female inflorescences (IFI) as explants. IFI excised from an adult tree formed multiple buds after 4-week incubation on Murashige and Skoog medium with 0.05 micromol 1-1 NAA and 11.1 micromol 1-1 BAP. The axillary buds evolved into 5-6 cm long shoots 5 weeks after the transfer of IFI on a similar medium except for the addition of activated charcoal. Rooting of the shoots was obtained on a third medium, without BAP or charcoal, but with 1 micromol 1-1 NAA. The plantlets were transferred into soil. Their growth was satisfactory and no plagiotropic tendency was observed. (Résumé d'auteur

lHuman cytotoxic T lymphocytes with reduced sensitivity to Fas-induced apoptosis

Effector-memory T cells expressing Fas (Apo-1/CD95) are switched to an apoptotic program by cross-linking with Fas-ligand (FasL). Consequently, tumors that express FasL can induce apoptosis of infiltrating Fas-positive T lymphocytes and subdue any antitumor host immune response. Since Epstein-Barr virus (EBV)-associated tumors such as Hodgkin lymphoma (HL) and nasopharyngeal carcinoma (NPC) express FasL, we determined whether EBV-specific cytotoxic T lymphocytes (EBV-CTLs) could be modified to resist this evasion strategy. We show that long-term down-modulation of Fas can be achieved in EBV-CTLs by transduction with small interfering RNA (siRNA) encoded in a retrovirus. Modified T cells resisted Fas/FasL-mediated apoptosis compared with control cells and showed minimal cleavage of the caspase3 substrate poly(ADP-ribose) polymerase (PARP) protein after Fas engagement. Prolonged Fas stimulation selected a uniformly Fas(low) and FasL resistant population. Removal of responsiveness to this single death signal had no other discernible effects on EBV-CTLs. In particular, it did not lead to their autonomous growth since the modified EBV-CTLs remained polyclonal, and their survival and proliferation retained dependence on antigen-specific stimulation and on the presence of other physiologic growth signals. EBV-CTLs with knocked down Fas should have a selective functional and survival advantage over unmodified EBV-CTLs in the presence of tumors expressing FasL and may be of value for adoptive cellular therapy. (c) 2005 by The American Society of Hematology

ASB2 is an Elongin BC-interacting protein that can assemble with Cullin 5 and Rbx1 to reconstitute an E3 ubiquitin ligase complex.

International audience; The ankyrin repeat-containing protein with a suppressor of cytokine signaling box-2 (ASB2) gene was identified as a retinoic acid-response gene and a target of the promyelocytic leukemia-retinoic acid receptor-alpha oncogenic protein characteristic of acute promyelocytic leukemia. Expression of ASB2 in myeloid leukemia cells inhibits growth and promotes commitment, recapitulating an early step known to be critical for differentiation. Here we show that ASB2, by interacting with the Elongin BC complex, can assemble with Cullin5.Rbx1 to form an E3 ubiquitin ligase complex that stimulates polyubiquitination by the E2 ubiquitin-conjugating enzyme Ubc5. This is a first indication that a member of the ASB protein family, ASB2, is a subunit of an ECS (Elongin C-Cullin-SOCS box)-type E3 ubiquitin ligase complex. Altogether, our results strongly suggest that ASB2 targets specific proteins to destruction by the proteasome in leukemia cells that have been induced to differentiate

H-theorem for classical matter around a black hole

We propose a classical solution for the kinetic description of matter falling into a black hole, which permits to evaluate both the kinetic entropy and the entropy production rate of classical infalling matter at the event horizon. The formulation is based on a relativistic kinetic description for classical particles in the presence of an event horizon. An H-theorem is established which holds for arbitrary models of black holes and is valid also in the presence of contracting event horizons

Integrated Planning of a Solar/Storage Collective

French regulation allows consumers in low-voltage networks to form collectives to produce, share, and consume local energy under the collective self-consumption framework. A natural consequence of collectively-owned generation projects is the need to allocate production among consumers. In long-term plans, production allocation determines each of the consumers' benefits of joining the collective. In the short-term, energy should be dynamically allocated to reflect operation. This paper presents a framework that integrates long and short-term planning of a collective that shares a solar plus energy storage system. In the long-term planning stage, we maximize the collective's welfare and equitably allocate expected energy to each consumer. For operation, we propose a model predictive control algorithm that minimizes short-term costs and allocates energy to each consumer on a 30-minute basis (as required by French regulation). We adjust the energy allotment ex-post operation to reflect the materialization of uncertainty. We present a case study where we showcase the framework for a 15 consumer collective.Comment: Final draft accepted for publication in IEEE Transactions on Smart Gri

Environmental fluctuations accelerate molecular evolution of thermal tolerance in a marine diatom

This is the final version of the article. Available from Springer Nature via the DOI in this recordThe publisher correction to this article is in ORE at: http://hdl.handle.net/10871/34487Diatoms contribute roughly 20% of global primary production, but the factors determining their ability to adapt to global warming are unknown. Here we quantify the capacity for adaptation to warming in the marine diatom Thalassiosira pseudonana. We find that evolutionary rescue under severe (32 °C) warming is slow, but adaptation to more realistic scenarios where temperature increases are moderate (26 °C) or fluctuate between benign and severe conditions is rapid and linked to phenotypic changes in metabolic traits and elemental composition. Whole-genome re-sequencing identifies genetic divergence among populations selected in the different warming regimes and between the evolved and ancestral lineages. Consistent with the phenotypic changes, the most rapidly evolving genes are associated with transcriptional regulation, cellular responses to oxidative stress and redox homeostasis. These results demonstrate that the evolution of thermal tolerance in marine diatoms can be rapid, particularly in fluctuating environments, and is underpinned by major genomic and phenotypic change.This study was funded by a Leverhulme Trust research grant (RPG-2013-335). Whole genome re-sequencing was carried out at Exeter Sequencing Service and Computational core facilities at the University of Exeter, where Dr. Karen Moore, Dr. Audrey Farbos, Paul O’Neill, and Dr. Konrad Paszkiewicz lead the handling of the samples. Exeter Squencing Services are supported by Medical Research Council Clinical Infrastructure award (MR/M008924/1), Wellcome Trust Institutional Strategic Support Fund (WT097835MF), Wellcome Trust Multi User Equipment Award (WT101650MA), and BBSRC LOLA award (BB/K003240/1)