123 research outputs found

    Experimental Measurement of Dolphin Thrust Generated during a Tail Stand Using DPIV

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    Estimation of force generated by dolphins has long been debated. The problem was that indirect estimates of force production for dolphins resulted in low values that could not be validated. Bubble digital particle image velocimetry (DPIV) measured hydrodynamic force production for swimming dolphins and demonstrated high force production. To validate the bubble DPIV and reconcile force production measurements, two bottlenose dolphins (Tursiops truncatus) performing tail stands were measured with bubble DPIV. Microbubbles were generated from a finely porous hose and compressed air source. Displacement of the bubbles by the propulsive motions of the dolphin was tracked with a high-speed video camera. Oscillations of the dolphin flukes generated strong vortices and a downward directed jet flow into the wake. Application of the Kutta–Joukowski theorem measuring vortex circulations yielded forces up to 997.3 N. Another video camera recorded body height above the water surface to determine the mass-force of the dolphin above the water surface. For the dolphin to hold its position above the water surface, the mass-force approximately balanced the vertical hydrodynamic force from the flukes. The results demonstrated the fluke motions generate high sustained forces roughly equal to the dolphin’s weight out of the water. Bubble DPIV validated high forces measured previously for thrust generated in swimming by animals and demonstrated a more accurate technique compared to standard aerodynamic analysis

    Experimental Measurement of Dolphin Thrust Generated during a Tail Stand Using DPIV

    Get PDF
    : Estimation of force generated by dolphins has long been debated. The problem was that indirect estimates of force production for dolphins resulted in low values that could not be validated. Bubble digital particle image velocimetry (DPIV) measured hydrodynamic force production for swimming dolphins and demonstrated high force production. To validate the bubble DPIV and reconcile force production measurements, two bottlenose dolphins (Tursiops truncatus) performing tail stands were measured with bubble DPIV. Microbubbles were generated from a finely porous hose and compressed air source. Displacement of the bubbles by the propulsive motions of the dolphin was tracked with a high-speed video camera. Oscillations of the dolphin flukes generated strong vortices and a downward directed jet flow into the wake. Application of the Kutta–Joukowski theorem measuring vortex circulations yielded forces up to 997.3 N. Another video camera recorded body height above the water surface to determine the mass-force of the dolphin above the water surface. For the dolphin to hold its position above the water surface, the mass-force approximately balanced the vertical hydrodynamic force from the flukes. The results demonstrated the fluke motions generate high sustained forces roughly equal to the dolphin’s weight out of the water. Bubble DPIV validated high forces measured previously for thrust generated in swimming by animals and demonstrated a more accurate technique compared to standard aerodynamic analysis

    Identification of Tp0751 (Pallilysin) as a Treponema pallidum Vascular Adhesin by Heterologous Expression in the Lyme disease Spirochete

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    Treponema pallidum subsp. pallidum, the causative agent of syphilis, is a highly invasive spirochete pathogen that uses the vasculature to disseminate throughout the body. Identification of bacterial factors promoting dissemination is crucial for syphilis vaccine development. An important step in dissemination is bacterial adhesion to blood vessel surfaces, a process mediated by bacterial proteins that can withstand forces imposed on adhesive bonds by blood flow (vascular adhesins). The study of T. pallidum vascular adhesins is hindered by the uncultivable nature of this pathogen. We overcame these limitations by expressing T. pallidum adhesin Tp0751 (pallilysin) in an adhesion-attenuated strain of the cultivable spirochete Borrelia burgdorferi. Under fluid shear stress representative of conditions in postcapillary venules, Tp0751 restored bacterial-vascular interactions to levels similar to those observed for infectious B. burgdorferi and a gain-of-function strain expressing B. burgdorferi vascular adhesin BBK32. The strength and stability of Tp0751- and BBK32-dependent endothelial interactions under physiological shear stress were similar, although the mechanisms stabilizing these interactions were distinct. Tp0751 expression also permitted bacteria to interact with postcapillary venules in live mice as effectively as BBK32-expressing strains. These results demonstrate that Tp0751 can function as a vascular adhesin

    Therapeutic Effects of Autologous Tumor-Derived Nanovesicles on Melanoma Growth and Metastasis

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    Cancer vaccines with optimal tumor-associated antigens show promise for anti-tumor immunotherapy. Recently, nano-sized vesicles, such as exosomes derived from tumors, were suggested as potential antigen candidates, although the total yield of exosomes is not sufficient for clinical applications. In the present study, we developed a new vaccine strategy based on nano-sized vesicles derived from primary autologous tumors. Through homogenization and sonication of tumor tissues, we achieved high yields of vesicle-bound antigens. These nanovesicles were enriched with antigenic membrane targets but lacked nuclear autoantigens. Furthermore, these nanovesicles together with adjuvant activated dendritic cells in vitro, and induced effective anti-tumor immune responses in both primary and metastatic melanoma mouse models. Therefore, autologous tumor-derived nanovesicles may represent a novel source of antigens with high-level immunogenicity for use in acellular vaccines without compromising safety. Our strategy is cost-effective and can be applied to patient-specific cancer therapeutic vaccination
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