Three-dimensional pore-scale modelling of dentinal infiltration

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Demineralized dentin 3 D porosity and pore size distribution using mercury porosimetry

ObjectivesThe objectives of this study were to assess demineralized dentin porosity and quantify the different porous features distribution within the material using mercury intrusion porosimetry (MIP) technique. We compared hexamethyldisilazane (HMDS) drying and lyophilization (LYO) (freeze-drying) in sample preparation.MethodsFifty-six dentin discs were assigned into three groups. The control (CTR) group discs were superficially acid-etched (15 s 37% H3PO4) to remove the smear layer and then freeze-dried whereas LYO and HMDS groups samples were first totally demineralized using EDTA 0.5 M and then freeze-dried and HMDS-dried respectively. MIP was used to determine open porosity and pore size distribution of each pair of samples. Field emission scanning electron microscopy (FESEM) was used to illustrate the results.ResultsThe results showed two types of pores corresponding either to tubules and micro-branches or to inter-fibrillar spaces created by demineralization. Global porosity varied from 59% (HMDS-dried samples) to 70% (freeze-dried samples). Lyophilization drying technique seems to lead to less shrinkage than HMDS drying. FESEM revealed that collagen fibers of demineralized lyophilized samples are less melted together than in the HMDS-dried samples.SignificanceDemineralized dentin porosity is a key parameter in dentin bonding that will influence the hybrid layer quality. Its characterization could be helpful to improve the monomers infiltration

Microfluidic model of porous media wetting ; application to a collagen network

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Characterization of deproteinized dentin for its use in bone tissue engineering

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Three Dimensional Characterization of the Dentin Porous Network Using Confocal Laser Scanning Microscopy (CLSM)

International audienceIn this paper, the 3D-morphology of the porous structure in dentin is investigated by confocal laser scanning microscopy (CLSM). The porous microstructure near the dentino-enamel junction (DEJ), which consists of tubules partly connected by lateral branches, was found to exhibit a complex geometry. We revisit and challenge previous 2D studies by focusing on the 3D morphology (tubule and branching geometry) and quantification of porosity. Our work provides fundamental insight into the microstructure of dentin which could be used to study the effect of age and pathologies. Furthermore, such information is essential for the design of biomaterials used in dentistry, e.g., to ensure more efficient bonding to dentin. Finally, our analysis of the tubular network structure provides valuable data that could be used directly as inputs in a numerical model

Using X-ray computed tomography for quantification of cell proliferation within a perfusion bioreactor

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Morphology and mechanics of dentin using FIB-SEM

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