Chemokine (C-C motif) ligand 2 mediates direct and indirect fibrotic responses in human and murine cultured fibrocytes

<p>Abstract</p> <p>Background</p> <p>Fibrocytes are a population of circulating bone-marrow-derived cells that express surface markers for leukocytes and mesenchymal cells, and are capable of differentiating into myofibroblasts. They have been observed at sites of active fibrosis and increased circulating numbers correlate with mortality in idiopathic pulmonary fibrosis (IPF). Inhibition of chemokine (C-C motif) receptor 2 (CCR2) during experimental models of lung fibrosis reduces lung collagen deposition, as well as reducing lung fibrocyte accumulation. The aim of the present study was to determine whether human and mouse fibrocytes express functional CCR2.</p> <p>Results</p> <p>Following optimized and identical human and murine fibrocyte isolation, both cell sources were shown to be positive for CCR2 by flow cytometry and this expression colocalized with collagen I and CD45. Human blood fibrocytes stimulated with the CCR2 ligand chemokine (C-C motif) ligand 2 (CCL2), demonstrated increased proliferation (<it>P </it>< 0.005) and differentiation into myofibroblasts (<it>P </it>< 0.001), as well as a chemotactic response (<it>P </it>< 0.05). Murine fibrocytes also responded to CCR2 stimulation, with CCL12 being more potent than CCL2.</p> <p>Conclusions</p> <p>This study directly compares the functional responses of human and murine fibrocytes to CCR2 ligands, and following comparable isolation techniques. We have shown comparable biological effects, strengthening the translatability of the murine models to human disease with respect to targeting the CCR2 axis to ameliorate disease in IPF patients.</p

Early evolution of the LIM homeobox gene family

Background: LIM homeobox (Lhx) transcription factors are unique to the animal lineage and have patterning roles during embryonic development in flies, nematodes and vertebrates, with a conserved role in specifying neuronal identity. Though genes of this family have been reported in a sponge and a cnidarian, the expression patterns and functions of the Lhx family during development in non-bilaterian phyla are not known

Assessing parallel gene histories in viral genomes

Background: The increasing abundance of sequence data has exacerbated a long known problem: gene trees and species trees for the same terminal taxa are often incongruent. Indeed, genes within a genome have not all followed the same evolutionary path due to events such as incomplete lineage sorting, horizontal gene transfer, gene duplication and deletion, or recombination. Considering conflicts between gene trees as an obstacle, numerous methods have been developed to deal with these incongruences and to reconstruct consensus evolutionary histories of species despite the heterogeneity in the history of their genes. However, inconsistencies can also be seen as a source of information about the specific evolutionary processes that have shaped genomes. Results: The goal of the approach here proposed is to exploit this conflicting information: we have compiled eleven variables describing phylogenetic relationships and evolutionary pressures and submitted them to dimensionality reduction techniques to identify genes with similar evolutionary histories. To illustrate the applicability of the method, we have chosen two viral datasets, namely papillomaviruses and Turnip mosaic virus (TuMV) isolates, largely dissimilar in genome, evolutionary distance and biology. Our method pinpoints viral genes with common evolutionary patterns. In the case of papillomaviruses, gene clusters match well our knowledge on viral biology and life cycle, illustrating the potential of our approach. For the less known TuMV, our results trigger new hypotheses about viral evolution and gene interaction. 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A 16 GBPS, Full-Duplex Transceiver over Lossy On-Chip Interconnects in 28 NM CMOS Technology

A high-speed full-duplex transceiver (FDT) over lossy on-chip interconnects is presented. The FDT employs a hybrid circuit to separate the inbound and outbound signals from each other and also performs echo-cancellation with the help of the main and the auxiliary drivers. A hybrid MOS device is utilized for impedance matching and conversion of the received voltage signal into a current signal for amplification. Moreover, a compensation capacitance ( Cc ) is used at the output of the main driver to minimize the residual echo signal and achieve a higher data rate. The entire FDT architecture has been designed in TSMC 28 nm CMOS standard process with 0.9 V supply voltage. The performance results validate a 16 Gbps FD operation with a root-mean-square (RMS) jitter of 16.4 ps, and a power efficiency of 0.16 pJ/b/mm over a 5 mm on-chip interconnect without significant effect due to process-voltage-temperature (PVT) variations. To the best knowledge of the authors, this work shows the highest achievable full-duplex data rate, among the solutions reported in the literature to date, yet with low complexity, low layout area of 1581 \u3bcm2 and competitive power efficiency

Design and implementation a microcontroller based high power ultrasonic dispersion system with self frequency adjusting property

In This paper a novel ultrasonic dispersion system for the cleaning application or dispersing of particles which are mixed in liquid, has been proposed. The frequency band of designed system is 28 kHz so that the frequency of ultrasonic wave sweeps from 20 kHz to 30 kHz with 100 Hz steps. Transferring a maximum and optimum energy of ultrasonic wave to the liquid conveyor with high efficiency during total usage time is remarkable superiority of designed and manufactured system compares with other similar available systems in markets. High power ultrasonic transducers with the nominal maximum power 110 watt are applied as the ultrasonic wave generator. To increase the efficiency of system and transmitting the maximum energy to the liquid tank, the frequency of system should set on the resonance frequency of transducer. Control section is implemented in system for realtime monitoring and adjusting the frequency of system. Hall Effect current sensor is used as the current sampling component and controlling program is implemented on AVR microcontrollers. The manufactured ultrasonic dispersion system is consisted of 4 high power ultrasonic transducer result in 450 watt ultrasonic energy, effectively