Hydrolysis of organic phosphorus in soil water suspensions after addition of phosphatase enzymes

Additions of enzymes involved in organic phosphorus (P) hydrolysis can be used to characterize the hydrolyzability of molybdate-unreactive P (MUP) in soil water extracts. Our aim was to test the feasibility of enzyme additions to soil water suspensions with respect to (1) suitable enzyme preparations and (2) recovery of molybdate-reactive P (MRP). To this end, we compared the substrate specificity of seven commercially available enzyme preparations (acid and alkaline phosphomonoesterase, phytase, and nuclease preparations) and optimized the assay conditions in microplates. We then measured MRP release after the addition of the enzymes to soil water suspensions and filtrates of two Swiss grassland soils (midland and alpine). In some cases, commercial preparations of the same enzyme differed in their specificity, presumably due to contamination with other enzymes, and also in their efficiency in soil suspensions. Addition of EDTA to the buffer was required to decrease sorption of released P in soil suspensions. Enzymatic release of P was consistently equal or higher in soil suspensions than in soil filtrates. However, also more dissolved MUP was present in soil suspensions than in filtrates, since the buffer interacted with the solid phase. Of the total dissolved MUP in soil suspensions, 94 and 61% were hydrolyzable in midland and alpine soil, respectively. More specifically, 60 and 17% of MUP were in nucleic acids, 6 and 39% in simple monoesters, and 28 and 5% in inositol hexakisphosphate in midland and alpine soil, respectively. Thus, we show that the characterization of hydrolyzable organic P in soil suspensions with hydrolytic enzyme preparations may be useful to better understand the availability of soil organic P to enzymatic hydrolysis, but that it requires soil-specific adaptation for optimum P recover

Monocyte biology conserved across species: Functional insights from cattle.

Similar to human monocytes, bovine monocytes can be split into CD14highCD16- classical, CD14highCD16high intermediate and CD14-/dimCD16high nonclassical monocytes (cM, intM, and ncM, respectively). Here, we present an in-depth analysis of their steady-state bulk- and single-cell transcriptomes, highlighting both pronounced functional specializations and transcriptomic relatedness. Bulk gene transcription indicates pro-inflammatory and antibacterial roles of cM, while ncM and intM appear to be specialized in regulatory/anti-inflammatory functions and tissue repair, as well as antiviral responses and T-cell immunomodulation. Notably, intM stood out by high expression of several genes associated with antigen presentation. Anti-inflammatory and antiviral functions of ncM are further supported by dominant oxidative phosphorylation and selective strong responses to TLR7/8 ligands, respectively. Moreover, single-cell RNA-seq revealed previously unappreciated heterogeneity within cM and proposes intM as a transient differentiation intermediate between cM and ncM

« Le problème, c’est qu’elle est mal baisée »: la lutte contre le sexisme en milieu médical menée par les étudiant·e·s en médecine [« Her problem is she hasn't been laid »: medical students fighting against sexist behaviours during training and clinical practice]

In 2018, a survey conducted by and among medical students in Lausanne revealed that they were witnesses and victims of numerous sexist behaviours during their training and clinical practice. The collected testimonies included accounts of sexual assault, sexual advances and microaggressions that have personal and professional impact on the victims. The CLASH was created with the aim of eradicating these incidents and changing the medical culture through the creation of an awareness-raising campaign, the implementation of a support hotline for victims and the introduction of a practical course during the medical curriculum. Further action at institutional and political level as well as large-scale studies are needed to ensure non-discriminatory training for all in the future

Photospheric and chromospheric activity in four young solar-type stars

We present a photometric and spectroscopic study of four G-K dwarfs, namely HD 166, epsilon Eri, chi1 Ori and kappa1 Cet. In three cases, we find a clear spatial association between photospheric and chromospheric active regions. For chi1 Ori we do not find appreciable variations of photospheric temperature, and chromospheric Halpha emission. We applied a spot/plage model to the observed rotational modulation of temperature and flux to derive spot/plage parameters and to reconstruct a rough three-dimensional map of the outer atmosphere of kappa1 Cet, HD 166 and epsilon Eri.Comment: 12 pages, 3 tables, 9 figures. Submitted to Ap

Improved Color-Temperature Relations and Bolometric Corrections for Cool Stars

We present new grids of colors and bolometric corrections for F-K stars having 4000 K < Teff < 6500 K, 0.0 < log g < 4.5 and -3.0 < [Fe/H] < 0.0. A companion paper extends these calculations into the M giant regime. Colors are tabulated for Johnson U-V and B-V; Cousins V-R and V-I; Johnson-Glass V-K, J-K and H-K; and CIT/CTIO V-K, J-K, H-K and CO. We have developed these color-temperature (CT) relations by convolving synthetic spectra with photometric filter-transmission-profiles. The synthetic spectra have been computed with the SSG spectral synthesis code using MARCS stellar atmosphere models as input. Both of these codes have been improved substantially, especially at low temperatures, through the incorporation of new opacity data. The resulting synthetic colors have been put onto the observational systems by applying color calibrations derived from models and photometry of field stars which have Teffs determined by the infrared-flux method. The color calibrations have zero points and slopes which change most of the original synthetic colors by less than 0.02 mag and 5%, respectively. The adopted Teff scale (Bell & Gustafsson 1989) is confirmed by the extraordinary agreement between the predicted and observed angular diameters of the field stars. We have also derived empirical CT relations from the field-star photometry. Except for the coolest dwarfs (Teff < 5000 K), our calibrated, solar-metallicity model colors are found to match these and other empirical relations quite well. Our calibrated, 4 Gyr, solar-metallicity isochrone also provides a good match to color-magnitude diagrams of M67. We regard this as evidence that our calibrated colors can be applied to many astrophysical problems, including modelling the integrated light of galaxies. (abridged)Comment: To appear in the March 2000 issue of the Astronomical Journal. 72 pages including 16 embedded postscript figures (one page each) and 6 embedded postscript tables (18 pages total

Coupling commercial fisheries and survey data: a practical solution to boost the amount of information in data-poor context

Quantitative fish stock assessment methods have become increasingly complex. However, the quality of available data may still restrict their applicability, being a particular concern in data-poor situations and where management decisions rely on either commercial fisheries or scientific survey data. In this study we address this issue by proposing a flexible statistical tool that can compare and integrate both datasets simultaneously, and hence boost the amount of information. Because of different sampling designs and procedures, distinct levels of biases arise between datatypes (e.g., different spatio-temporal coverages and size spectra of fish), which are accounted for in our model framework. The model is developed in Template Model Builder, alternatively applied to (i) commercial data, (ii) survey data and (iii) commercial coupled to survey data, and tested on cod, plaice and sprat stocks in the western Baltic Sea (2005-2016). We found that each data type supplied different, yet complementary, information on the species spatio-temporal dynamics. Though the overall spatial pattern in both datatypes showed similar trends, the variability was clearly higher when evaluating the datasets separately, while the coupled dataset was the most informative one. This confirms that the predictive modelling was greatly improved by joining the datasets and will likely enhance future stock evaluation and management advice in both data-poor and data-rich contexts. Moreover, our benchmark tool represents a valuable solution for supporting a robust bio-economic management of fisheries, and enhances the picture we have in data-poor context with spatial and temporal scales that really matters to fisheries policy makers

Structure of the outer layers of cool standard stars

Context: Among late-type red giants, an interesting change occurs in the structure of the outer atmospheric layers as one moves to later spectral types in the Hertzsprung-Russell diagram: a chromosphere is always present, but the coronal emission diminishes and a cool massive wind steps in. Aims: Where most studies have focussed on short-wavelength observations, this article explores the influence of the chromosphere and the wind on long-wavelength photometric measurements. Methods: The observational spectral energy distributions are compared with the theoretical predictions of the MARCS atmosphere models for a sample of 9 K- and M-giants. The discrepancies found are explained using basic models for flux emission originating from a chromosphere or an ionized wind. Results: For 7 out of 9 sample stars, a clear flux excess is detected at (sub)millimeter and/or centimeter wavelengths. The precise start of the excess depends upon the star under consideration. The flux at wavelengths shorter than about 1 mm is most likely dominated by an optically thick chromosphere, where an optically thick ionized wind is the main flux contributor at longer wavelengths. Conclusions: Although the optical to mid-infrared spectrum of the studied K- and M-giants is well represented by a radiative equilibrium atmospheric model, the presence of a chromosphere and/or ionized stellar wind at higher altitudes dominates the spectrum in the (sub)millimeter and centimeter wavelength ranges. The presence of a flux excess also has implications on the role of these stars as fiducial spectrophotometric calibrators in the (sub)millimeter and centimeter wavelength range.Comment: 13 pages, 6 figures, 7 pages of online material, submitted to A&

An XMM-Newton view of the young open cluster NGC 6231 -- II. The OB star population

In this second paper, we pursue the analysis of the 180 ks XMM-Newton campaign towards the young open cluster NGC 6231 and we focus on its rich OB star population. We present a literature-based census of the OB stars in the field of view with more than one hundred objects, among which 30% can be associated with an X-ray source. All the O-type stars are detected in the X-ray domain as soft and reasonably strong emitters. In the 0.5-10.0 keV band, their X-ray luminosities scale with their bolometric luminosities as $\log L_\mathrm{X} - \log L_\mathrm{bol}=-6.912\pm0.153$. Such a scaling law holds in the soft (0.5-1.0 keV) and intermediate (1.0-2.5 keV) bands but breaks down in the hard band. While the two colliding wind binaries in our sample clearly deviate from this scheme, the remaining O-type objects show a very limited dispersion (40% or 20% according to whether `cool' dwarfs are included or not), much smaller than that obtained from previous studies. At our detection threshold and within our sample, the sole identified mechanism that produces significant modulations in the O star X-ray emission is related to wind interaction. The intrinsic X-ray emission of non-peculiar O-type stars seems thus constant for a given star and the level of its X-ray emission is accurately related to the its luminosity or, equivalently, to its wind properties. Among B-type stars, the detection rate is only about 25% in the sub-type range B0-B4 and remains mostly uniform throughout the different sub-populations while it drops significantly at later sub-types. The associated X-ray spectra are harder than those of O-type stars. Our analysis points towards the detected emission being associated with a physical PMS companion >... [see paper for the complete abstract]Comment: 21 pages, 14 figures, Table 2 and Figs 2 to 5 will be available through the CDS only, accepted for publication by MNRAS, Fig 1 not included in the present preprint because of size limitation