26 research outputs found
Analyse des relations entre Dysaphis plantaginea Pass. (insecte homoptère) et sa plante hôte Malus x domestica Borkh.: étude de la résistance du cultivar Florina
*INRA, centre d'Angers Diffusion du document : INRA, centre d'Angers Diplôme : Dr. d'Universit
Hérédité de la résistance du cv. Florina au puceron cendré du pommier Dysaphis plantaginea Pass.
National audienc
Résistance du cultivar "Florina-Quérina" au puceron cendré du pommier Dysaphis plantaginea (pass.)
National audienc
Lutte biologique contre la teigne des crucifères P. xylostella L. sur choux porte-graine
Vu les difficultés de la lutte chimique contre la teigne des crucifères Plutella xylostella sur chou, particuluèrement en cultures porte-graine (exigences autour de la pollinisation, cahier des charges des semences pour l' agriculture biologique AB), des essais de production biologique sont menés. Des souches de trois espèces de trichogrammes ont été testées en serre de producteur en AB en Pays-de-la-Loire, grâce à la technique des "choux élevage" (explicitée dans l' article). Trichogramma evanescens et T. ostriniae semblent plus intéressants que T. semblidis. Par ailleurs, la densité de végétation semble jouer. des études complémentaires sont nécessaires et envisagées
Fine mapping of the rosy apple aphid resistance locus Dp-fl on linkage group 8 of the apple cultivar ‘Florina’
Rosy apple aphid (Dysaphis plantaginea), is one of the major insect pests of apple, causing serious physical and economic damage to fruit production. A dominant resistance gene Dp-fl was previously mapped at the bottom of linkage group LG8 from the cultivar ‘Florina’, linked to the SSR CH01h10. The development of additional genetic markers mapping closer to Dp-fl was needed to position the gene accurately and to improve the effectiveness of marker-assisted breeding (MAB). The aims of this study were to identify single nucleotide polymorphisms (SNPs) in the region of Dp-fl and to position these SNPs relative to Dp-fl. To generate a fine map of the Dp-fl interval, a total of 191 plants segregating for resistance and derived from four different populations were tested with temperature-switch PCR (TSP) markers developed for SNPs located in the region of CH01h10. All the plants were phenotypically evaluated for aphid resistance and those data compared with the genetic data. These efforts resulted in positioning the Dp-fl resistance locus in a genetic interval corresponding to a physical distance of about 330 kb on the ‘Golden Delicious’ genome. The new markers were tested on several apple founder cultivars in order to test the specificity of the SNPs and, thus, the best markers for the MAB were identified. Finally, the 330-kb interval was analyzed for the identification of coding sequences and putative candidate genes for D. plantaginea resistance were identified