Equine or porcine synovial fluid as a novel ex vivo model for the study of bacterial free-floating biofilms that form in human joint infections

Bacterial invasion of synovial joints, as in infectious or septic arthritis, can be difficult to treat in both veterinary and human clinical practice. Biofilms, in the form of free-floating clumps or aggregates, are involved with the pathogenesis of infectious arthritis and periprosthetic joint infection (PJI). Infection of a joint containing an orthopedic implant can additionally complicate these infections due to the presence of adherent biofilms. Because of these biofilm phenotypes, bacteria within these infected joints show increased antimicrobial tolerance even at high antibiotic concentrations. To date, animal models of PJI or infectious arthritis have been limited to small animals such as rodents or rabbits. Small animal models, however, yield limited quantities of synovial fluid making them impractical for in vitro research. Herein, we describe the use of ex vivo equine and porcine models for the study of synovial fluid induced biofilm aggregate formation and antimicrobial tolerance. We observed Staphylococcus aureus and other bacterial pathogens adapt the same biofilm aggregate phenotype with significant antimicrobial tolerance in both equine and porcine synovial fluid, analogous to human synovial fluid. We also demonstrate that enzymatic dispersal of synovial fluid aggregates restores the activity of antimicrobials. Future studies investigating the interaction of bacterial cell surface proteins with host synovial fluid proteins can be readily carried out in equine or porcine ex vivo models to identify novel drug targets for treatment of prevention of these difficult to treat infectious diseases

Kepler Eclipsing Binary Stars. V. Identification of 31 Eclipsing Binaries in the K2 Engineering Data-set

Over 2500 eclipsing binaries were identified and characterized from the ultra-precise photometric data provided by the Kepler space telescope. Kepler is now beginning its second mission, K2, which is proving to again provide ultra-precise photometry for a large sample of eclipsing binary stars. In the 1951 light curves covering 12 days in the K2 engineering data-set, we have identified and determined the ephemerides for 31 eclipsing binaries that demonstrate the capabilities for eclipsing binary science in the upcoming campaigns in K2. Of those, 20 are new discoveries. We describe both manual and automated approaches to harvesting the complete set of eclipsing binaries in the K2 data, provide identifications and details for the full set of eclipsing binaries present in the engineering data-set, and discuss the prospects for application of eclipsing binary searches in the K2 mission.Comment: 12 pages, 2 figures, submitted to PAS

An evaluation of the effectiveness of a reproductive health education program for nonmedical students in Iran: A quasi-experimental pre-test, post-test research

Background: Young people age 10 - 25 are an important population to evaluate the female youth educational program essential to the prevention of issues related to reproductive health. Objectives: This study evaluated the effectiveness of the education program for improving university student�s reproductive health. Patients and Methods: This quantitative study was conducted in Iran from July 2014 to March 2015. The questionnaire addressed socio-demographic features, knowledge of reproductive problems, and attitudes. Program effectiveness was examined using a quasi-experimental research design with pre-intervention and post-intervention. Data from questionnaires was collected preintervention and post-intervention from 150 female students. Results: In this study, results showed that an intervention and education program had a significant effect on several issues related to reproductive health. Most participants stressed the need to provide reproductive health services for young girls. Conclusions: The results of this study suggest that the reproductive health education program improved the students� knowledge and attitudes about sexuality and decision-making after the program and that these educational programs are important for youths. © 2016, Shiraz University of Medical Sciences

New Attractors, Entropy Function and Black Hole Partition Function

By making use of the entropy function formalism we study the generalized attractor equations in the four dimensional N=2 supergravity in presence of higher order corrections. This result might be used to understand a possible ensemble one could associate to an extremal black hole.Comment: 20 pages, latex file, V2: minor corrections, typos corrected, Refs added, V3: the version appeared in JHE

Microstructure and corrosion of AA2024

AA2024-Tx is one of the most common high-strength aluminium alloys used in the aerospace industry. This article reviews current understanding of the microstructure of sheet AA2024-T3 and chronicles the emergence of new compositions for constituent particles as well as reviews older literature to understand the source of the original compositions. The review goes on to summarise older and more recent studies on corrosion of AA2024-T3, drawing attention to areas of corrosion initiation and propagation. It pays particular attention to modern approaches to corrosion characterisation as obtained through microelectrochemical techniques and physicochemical characterisation, which provide statistical assessment of factors that contribute to corrosion of AA2024. These approaches are also relevant to other alloys

Genome-wide analysis reveals distinct global populations of pink bollworm (Pectinophora gossypiella)

The pink bollworm (Pectinophora gossypiella) is one of the world's most destructive pests of cotton. This invasive lepidopteran occurs in nearly all cotton-growing countries. Its presence in the Ord Valley of North West Australia poses a potential threat to the expanding cotton industry there. To assess this threat and better understand population structure of pink bollworm, we analysed genomic data from individuals collected in the field from North West Australia, India, and Pakistan, as well as from four laboratory colonies that originated in the United States. We identified single nucleotide polymorphisms (SNPs) using a reduced-representation, genotyping-by-sequencing technique (DArTseq). The final filtered dataset included 6355 SNPs and 88 individual genomes that clustered into five groups: Australia, India-Pakistan, and three groups from the United States. We also analysed sequences from Genbank for mitochondrial DNA (mtDNA) locus cytochrome c oxidase I (COI) for pink bollworm from six countries. We found low genetic diversity within populations and high differentiation between populations from different continents. The high genetic differentiation between Australia and the other populations and colonies sampled in this study reduces concerns about gene flow to North West Australia, particularly from populations in India and Pakistan that have evolved resistance to transgenic insecticidal cotton. We attribute the observed population structure to pink bollworm's narrow host plant range and limited dispersal between continents

N=1/2 Super Yang-Mills Theory on Euclidean AdS2xS2

We study D-branes in the background of Euclidean AdS2xS2 with a graviphoton field turned on. As the background is not Ricci flat, the graviphoton field must have both self-dual and antiself-dual parts. This, in general, will break all the supersymmetries on the brane. However, we show that there exists a limit for which one can restore half of the supersymmetries. Further, we show that in this limit, the N=1/2 SYM Lagrangian on flat space can be lifted on to the Euclidean AdS2xS2 preserving the same amount of supersymmetries as in the flat case. We observe that without the C-dependent terms present in the action this lift is not possible.Comment: 12 pages, latex file; v2: minor corrections, references adde

Impregnation of bone chips with antibiotics and storage of antibiotics at different temperatures: an in vitro study

<p>Abstract</p> <p>Background</p> <p>Allograft bone used in joint replacement surgery can additionally serve as a carrier for antibiotics and serve as a prophylaxis against infections. However, <it>in vitro </it>dose-response curves for bone chips impregnated with different kinds of antibiotics are not available. In addition, while it would be desirable to add the antibiotics to allograft bone chips before these are stored in a bone bank, the effects of different storage temperatures on antibiotics are unknown.</p> <p>Methods</p> <p>Five different antibiotics (cefazolin, clindamycin, linezolid, oxacillin, vancomycin) were stored, both as pills and as solutions, at -80°C, -20°C, 4°C, 20°C and 37°C; in addition, bone chips impregnated with cefazolin and vancomycin were stored at -80°C and -20°C. After 1 month, 6 months and 1 year, the activity of the antibiotics against <it>Staphylococcus epidermidis </it>was measured using an inoculated agar. The diameter of the <it>S. epidermidis</it>-free zone was taken as a measure of antibiotic activity.</p> <p>In a separate experiment, <it>in vitro </it>dose-response curves were established for bone chips impregnated with cefazolin and vancomycin solutions at five different concentrations.</p> <p>Finally, the maximum absorbed amounts of cefazolin and vancomycin were established by impregnating 1 g of bone chips with 5 ml of antibiotic solution.</p> <p>Results</p> <p>A decrease of the <it>S. epidermidis</it>-free zone was seen with oxacillin and cefazolin solutions stored at 37°C for 1 month, with vancomycin stored at 37°C for 6 months and with cefazolin and oxacillin solutions stored at 20°C for 6 months. The activity of the other antibiotic solutions, pills and impregnated bone chips was not affected by storage. The <it>in vitro </it>dose-response curves show that the free-zone diameter increases logarithmically with antibiotic concentration. The absorbed antibiotic amount of one gram bone chips was determined.</p> <p>Conclusions</p> <p>Storage of antibiotics in frozen form or storage of antibiotic pills at temperatures up to 37°C for 12 months does not affect their activity. However, storage of antibiotic solutions at temperatures above 20°C does affect the activity of some of the antibiotics investigated. The <it>in vitro </it>dose-response curve can be used to determine the optimal concentration(s) for local application. It provides the opportunity to determine the antibiotic content of bone chips, and thus the amount of antibiotics available locally after application.</p