Synthetic Medium for Growing Single Cells of Tobacco

1. Studies were done to find out a synthetic defined medium for growing single cells of tobacco. Two kinds of media were tried for these purposes. With several changes in components, the revised medium of LINSMAIER & SKOOG (6) seemed to be good lot suspension and plating cultures of tobacco cells. The medium used by ERARLE & TORREY (3) for cells of Convolvulus also gave the desired results (table 1, plate 3).2. Growth of both callus and single cell cultures occurred on comparable kinetin concentrations.3. These studies (about the relationship between IAA and kinetin) gave no evidence of differentiation of the cell colonies within seven weeks after transferring the cells into the medium (plate 5).

Induction of Somatic Embryos in Cultured Leaf Explants of Coffea Arabica

Sari. Tulisan ini menguraikan induksi embrio somatik dan pertumbuhan planlet yang dihasilkan dari kultur jaringan daun Coffea arabica. Untuk pembentukan kalus, potongan jaringan daun ditanam pada medium Linsmaier dan Skoog dengan 3% sukrosa ditambah berbagai konsentrasi 2,4-asam diklorofenoksi asetat atau asam naftalen asetat dan kinetin. Delapan belas minggu setelah penanaman, kalus akan dibentuk pada medium Linsmaier dan Skoog dengan 2 pM 2,4-asam diklorofenoksi asetat dan 5-7 pM kinetin dan juga pada medium dengan 0,05 pM asam naftalen asetat dan 6-8 pM kinetin. Pembentukan embrio somatic diperoleh dengan pemotongan kalus pada medium padat Linsmaier dan Skoog dengan 3 pM sampai 35 pM kinetin dan 0,05 pM asam indole butirat dan juga dalam medium cair Gamborg, Miller, dan Ojima yang diberi 0,05 pM sampai 2,5 pM 2,4-asam diklorofenoksi asetat. Abstract. Somatic embryo induction and subsequent plantlets development in culture of Coffea arabica leaf tissue explants was described. For callus formation leaf segments were grown on medium Linsmaier and Skoog with 3% sucrose and varying concentrations of 2,4-Dichlorophenoxyaceticacid or Naphthalene Acetic Acid and Kinetin. Eighteen weeks after inoculation, callus will be formed on Linsmaier and Skoog's medium with 2 μM 2,4-Dichlorophenoxyacetic acid and 5-7 μM Kinetin and also on medium with 0.05 μM Naphtalene Acetic Acid and 6-8 μM Kinetin. Somatic embryos are then formed by inoculation of calli segments on medium Linsmaier & Skoog with 3 μM to 35 μM Kinetin and 0.05 μM Indole Butyric Acid. Somatic embryos are also formed in liquid medium of Gamborg, Miller and Ojima supplemented with 0.05 μM to 2.5 μM 2,4-Dichlorophenoxyacetic acid

Relationships between anopheline mosquitoes and topography in West Timor and Java, Indonesia

<p>Abstract</p> <p>Background</p> <p>Malaria is a serious health issue in Indonesia. Mosquito control is one aspect of an integrated malaria management programme. To focus resources on priority areas, information is needed about the vectors and their habitats. This research aimed to identify the relationship between anopheline mosquitoes and topography in West Timor and Java.</p> <p>Methods</p> <p>Study areas were selected in three topographic types in West Timor and Java. These were: coastal plain, hilly (rice field) and highland. Adult mosquitoes were captured landing on humans identified to species level and counted.</p> <p>Results</p> <p>Eleven species were recorded, four of which were significant for malaria transmission: <it>Anopheles aconitus, Anopheles barbirostris, Anopheles subpictus </it>and <it>Anopheles sundaicus</it>. Each species occupied different topographies, but only five were significantly associated: <it>Anopheles annularis, Anopheles vagus </it>and <it>Anopheles subpictus </it>(Java only) with hilly rice fields; <it>Anopheles barbirostris, Anopheles maculatus </it>and <it>Anopheles subpictus </it>(West Timor only) with coastal areas.</p> <p>Conclusion</p> <p>Information on significant malaria vectors associated with specific topography is useful for planning the mosquito control aspect of malaria management.</p

Induction of Somatic Embryos in Cultured Leaf Explants of Coffea Arabica

Sari. Tulisan ini menguraikan induksi embrio somatik dan pertumbuhan planlet yang dihasilkan dari kultur jaringan daun Coffea arabica. Untuk pembentukan kalus, potongan jaringan daun ditanam pada medium Linsmaier dan Skoog dengan 3% sukrosa ditambah berbagai konsentrasi 2,4-asam diklorofenoksi asetat atau asam naftalen asetat dan kinetin. Delapan belas minggu setelah penanaman, kalus akan dibentuk pada medium Linsmaier dan Skoog dengan 2 pM 2,4-asam diklorofenoksi asetat dan 5-7 pM kinetin dan juga pada medium dengan 0,05 pM asam naftalen asetat dan 6-8 pM kinetin. Pembentukan embrio somatic diperoleh dengan pemotongan kalus pada medium padat Linsmaier dan Skoog dengan 3 pM sampai 35 pM kinetin dan 0,05 pM asam indole butirat dan juga dalam medium cair Gamborg, Miller, dan Ojima yang diberi 0,05 pM sampai 2,5 pM 2,4-asam diklorofenoksi asetat. Abstract. Somatic embryo induction and subsequent plantlets development in culture of Coffea arabica leaf tissue explants was described. For callus formation leaf segments were grown on medium Linsmaier and Skoog with 3% sucrose and varying concentrations of 2,4-Dichlorophenoxyaceticacid or Naphthalene Acetic Acid and Kinetin. Eighteen weeks after inoculation, callus will be formed on Linsmaier and Skoog's medium with 2 μM 2,4-Dichlorophenoxyacetic acid and 5-7 μM Kinetin and also on medium with 0.05 μM Naphtalene Acetic Acid and 6-8 μM Kinetin. Somatic embryos are then formed by inoculation of calli segments on medium Linsmaier &amp; Skoog with 3 μM to 35 μM Kinetin and 0.05 μM Indole Butyric Acid. Somatic embryos are also formed in liquid medium of Gamborg, Miller and Ojima supplemented with 0.05 μM to 2.5 μM 2,4-Dichlorophenoxyacetic acid