THE CONTRIBUTION OF SHORT-TERM MEMORY, WORKING MEMORY AND DEMOGRAPHIC CHARACTERISTICS TO THE LINGUISTIC ACHIEVEMENT OF GREEK MIDDLE SCHOOL-AGE CHILDREN: IMPLICATIONS FOR NATIVE LANGUAGE DEVELOPMENT

The present study delved into the involvement of Greek-speaking middle primary school children’s mnemonic recourses and demographic features in their performance in language tests. One hundred and seventy-six students aged 8.50-12.05 (mean age = 10.11 years) participated. The native language of the sample differed from English, in which most investigations have been conducted. Children’s verbal short-term memory (STM) and working memory (WM) capacity were assessed with a simple verbal retention measure (straight digit recall) and a task requiring parallel storage and processing of verbal information (backward digit recall), respectively. Pupils’ vocabulary knowledge was estimated through an assignment of expressive vocabulary. Children were also administered a reading fluency and a reading comprehension task. Pearson’s r and Spearman’s rho correlation coefficients showed that students’ verbal STM and WM significantly correlated with their reading fluency and text comprehension, accordingly. In addition, by employing simple linear regression analysis, increase in participants’ age was found to predict their extended reading fluency, while one-way Analysis of Variance (ANOVA) of independent samples revealed that vocabulary knowledge varied, being subject to pupils’ school grade. Lastly, independent samples t-test demonstrated no substantial difference in reading fluency between the two genders of children. Current findings are discussed in relation to participants’ age growth, their cumulating linguistic experience and the historical properties of the Greek language, indicating the need for further research that takes into account the unique and multifaceted nature of readers’ profile and outlining a few connotations for literacy instruction.  Article visualizations

A Human Recombinant Autoantibody-Based Immunotoxin Specific for the Fetal Acetylcholine Receptor Inhibits Rhabdomyosarcoma Growth In Vitro and in a Murine Transplantation Model

Rhabdomyosarcoma (RMS) is the most common malignant soft tissue tumor in children and is highly resistant to all forms of treatment currently available once metastasis or relapse has commenced. As it has recently been determined that the acetylcholine receptor (AChR) γ-subunit, which defines the fetal AChR (fAChR) isoform, is almost exclusively expressed in RMS post partum, we recombinantly fused a single chain variable fragment (scFv) derived from a fully human anti-fAChR Fab-fragment to Pseudomonas exotoxin A to generate an anti-fAChR immunotoxin (scFv35-ETA). While scFv35-ETA had no damaging effect on fAChR-negative control cell lines, it killed human embryonic and alveolar RMS cell lines in vitro and delayed RMS development in a murine transplantation model. These results indicate that scFv35-ETA may be a valuable new therapeutic tool as well as a relevant step towards the development of a fully human immunotoxin directed against RMS. Moreover, as approximately 20% of metastatic malignant melanomas (MMs) display rhabdoid features including the expression of fAChR, the immunotoxin we developed may also prove to be of significant use in the treatment of these more common and most often fatal neoplasms

A Novel Immunological Assay for Hepcidin Quantification in Human Serum

Contains fulltext : 81054.pdf (publisher's version ) (Open Access)BACKGROUND: Hepcidin is a 25-aminoacid cysteine-rich iron regulating peptide. Increased hepcidin concentrations lead to iron sequestration in macrophages, contributing to the pathogenesis of anaemia of chronic disease whereas decreased hepcidin is observed in iron deficiency and primary iron overload diseases such as hereditary hemochromatosis. Hepcidin quantification in human blood or urine may provide further insights for the pathogenesis of disorders of iron homeostasis and might prove a valuable tool for clinicians for the differential diagnosis of anaemia. This study describes a specific and non-operator demanding immunoassay for hepcidin quantification in human sera. METHODS AND FINDINGS: An ELISA assay was developed for measuring hepcidin serum concentration using a recombinant hepcidin25-His peptide and a polyclonal antibody against this peptide, which was able to identify native hepcidin. The ELISA assay had a detection range of 10-1500 microg/L and a detection limit of 5.4 microg/L. The intra- and interassay coefficients of variance ranged from 8-15% and 5-16%, respectively. Mean linearity and recovery were 101% and 107%, respectively. Mean hepcidin levels were significantly lower in 7 patients with juvenile hemochromatosis (12.8 microg/L) and 10 patients with iron deficiency anemia (15.7 microg/L) and higher in 7 patients with Hodgkin lymphoma (116.7 microg/L) compared to 32 age-matched healthy controls (42.7 microg/L). CONCLUSIONS: We describe a new simple ELISA assay for measuring hepcidin in human serum with sufficient accuracy and reproducibility

The 4C5 Cell-Impermeable Anti-HSP90 Antibody with Anti-Cancer Activity, Is Composed of a Single Light Chain Dimer

MAb 4C5 is a cell impermeable, anti-HSP90 murine monoclonal antibody, originally produced using hybridoma technology. We have previously shown that mAb 4C5 specifically recognizes both the α- and to a lesser extent the β-isoform of HSP90. Additionally, in vitro and in vivo studies revealed that by selectively inhibiting the function of cell-surface HSP90, mAb 4C5 significantly impairs cancer cell invasion and metastasis. Here we describe the reconstitution of mAb 4C5 into a mouse-human chimera. More importantly we report that mAb 4C5 and consequently its chimeric counterpart are completely devoid of heavy chain and consist only of a functional kappa light chain dimer. The chimeric antibody is shown to retain the original antibody's specificity and functional properties. Thus it is capable of inhibiting the function of surface HSP90, leading to reduced cancer cell invasion in vitro. Finally, we present in vivo evidence showing that the chimeric 4C5 significantly inhibits the metastatic deposit formation of MDA-MB-453 cells into the lungs of SCID mice. These data suggest that a chimeric kappa light chain antibody could be potentially used as an anti-cancer agent, thereby introducing a novel type of antibody fragment, with reduced possible adverse immunogenic effects, into cancer therapeutics

Type 2 Diabetes and Mediterranean Diet in Older Adults: a Brief Review of the Evidence

Purpose of Review: Mediterranean diet is a traditional, plant-based dietary pattern extensively studied for its favorable health effects. In relation to type 2 diabetes (T2D), higher adherence to the Mediterranean dietary pattern is associated with a lower risk for incidence of diabetes, better glycemic control, and improvements in cardiovascular risk factors in diabetic patients. Recent Findings: Interestingly, most of the studies on the relationship between T2D and adherence to the Mediterranean diet have focused on younger people, whereas the available evidence in older adults (≥ 60 years old) is limited. In this age group, the quality of the diet may be affected by factors that are associated with aging, including cognitive decline, whereas T2D has been associated with an elevated risk of cognitive deficits. More studies are needed in this age group in order to further investigate the potentially crucial role of T2D and glycemic control on cognition and their relationship with Mediterranean diet and to define the underlying mechanisms. Summary: In any case, healthcare professionals should educate older adults in adopting the basic principles of the Mediterranean diet or of other plant-based dietary patterns, as a diet promoting cognitive and cardiovascular health. © 2020, Springer Science+Business Media, LLC, part of Springer Nature

Isolation of potent human Fab fragments against a novel highly immunogenic region on human muscle acetylcholine receptor which protect the receptor from myasthenic autoantibodies.

In the autoimmune disease myasthenia gravis (MG), antibodies against the muscle nicotinic acetylcholine receptor (AChR) cause loss of functional AChR in the neuromuscular junction. To isolate AChR-specific human antibody fragments (Fab), a phage-display library was constructed from an MG patient's thymic B lymphocytes. The first Fab isolated had a low affinity for human AChR, but two sequential antibody chain shufflings using the MG donor heavy and light chain gene repertoires resulted in isolating two new Fab with an approximately 30-fold higher binding ability. The selected Fab contained extensively mutated heavy and light chains and probably represent intraclonal variants of a common progenitor having diverged in vivo by somatic hypermutation. Interestingly, the isolated Fab bound to an extracellular highly immunogenic region located either on an alpha-subunit site affected by the gamma/epsilon-subunits or on the interface between alpha- and gamma/epsilon-subunits. This region is not the previously described "main immunogenic region" (MIR), although it seems to be close to it, as one improved Fab and an anti-MIR mAb competed for AChR binding with distinctly different subpopulations of MG sera. Furthermore, this Fab protected surface AChR in cell cultures against MG autoantibody-induced antigenic modulation, suggesting a potential therapeutic use in MG, especially in combination with a human anti-MIR Fab

Eating habits and behaviors of older people: Where are we now and where should we go?

Nutrition is a modifiable factor affecting the quality of life and independence of older people. The physiological, psychological and social changes during aging affect their dietary choices. Many older adults have inadequate energy and protein intake. Carbohydrate intake and intake of total lipids, in terms of contribution to total energy intake, generally are within the recommended levels, but a decline in overall energy intake as well as the limited variety of micronutrient-dense foods that older people tend to consume result in an inadequate intake of several micronutrients. Adherence to healthy dietary patterns has been described as only moderate among older adults. Health-care practitioners should educate older people and promote healthy diets, in particular adequate energy and protein intake. © 2018 Elsevier B.V

Social life characteristics in relation to adherence to the Mediterranean diet in older adults: Findings from the Hellenic Longitudinal Investigation of Aging and Diet (HELIAD) study

Objective: The present study aimed to explore the associations between social life and adherence to a healthy dietary pattern, the Mediterranean diet (MD), in a population-representative cohort of older people.Design: Cross-sectional study. Adherence to the MD was evaluated by an a priori score; tertiles of the score, indicating low, medium and high adherence, were used in the analyses. Social life was assessed by a questionnaire evaluating participation in leisure-time activities and the number of social contacts; primary occupation was also recorded and job characteristics were further explored.Setting: Community-dwelling older adults.Participants: Adults from the Hellenic Longitudinal Investigation of Aging and Diet (HELIAD) study (n 1933; age range 65-99 years).Results: Each unit increase in the number of social contacts/month and in the frequency score of intellectual, social and physical activities was associated with a 1·6, 6·8, 4·8 and 13·7 % increase in the likelihood of a participant being in the high MD adherence group, respectively. The analysis by age group revealed that younger elderly participants had a 1·4, 8·4 and 11·3 % higher likelihood to be in the high adherence group for each unit increase in the number of social contacts/month and in the frequency score of engagement in intellectual and physical activities, respectively. Similar associations were found for older elderly participants with high compared with low MD adherence, except for the intellectual activities.Conclusions: The present results suggest that high MD adherence is associated with good social life, suggesting a clustering of health-promoting lifestyle factors in older adults. © 2019 The Authors