Equilibrium binding energies from fluctuation theorems and force spectroscopy simulations

Brownian dynamics simulations are used to study the detachment of a particle from a substrate. Although the model is simple and generic, we attempt to map its energy, length and time scales onto a specific experimental system, namely a bead that is weakly bound to a cell and then removed by an optical tweezer. The external driving force arises from the combined optical tweezer and substrate potentials, and thermal fluctuations are taken into account by a Brownian force. The Jarzynski equality and Crooks' fluctuation theorem are applied to obtain the equilibrium free energy difference between the final and initial states. To this end, we sample non--equilibrium work trajectories for various tweezer pulling rates. We argue that this methodology should also be feasible experimentally for the envisioned system. Furthermore, we outline how the measurement of a whole free energy profile would allow the experimentalist to retrieve the unknown substrate potential by means of a suitable deconvolution. The influence of the pulling rate on the accuracy of the results is investigated, and umbrella sampling is used to obtain the equilibrium probability of particle escape for a variety of trap potentials.Comment: 21 pages, 11 figures, To appear in Soft Matte

Polar orbit electrostatic charging of objects in shuttle wake

A survey of DMSP data has uncovered several cases where precipitating auroral electron fluxes are both sufficiently intense and energetic to charge spacecraft materials such as teflon to very large potentials in the absence of ambient ion currents. Analytical bounds are provided which show that these measured environments can cause surface potentials in excess of several hundred volts to develop on objects in the orbiter wake for particular vehicle orientations

Obtaining Stiffness Exponents from Bond-diluted Lattice Spin Glasses

Recently, a method has been proposed to obtain accurate predictions for low-temperature properties of lattice spin glasses that is practical even above the upper critical dimension, $d_c=6$. This method is based on the observation that bond-dilution enables the numerical treatment of larger lattices, and that the subsequent combination of such data at various bond densities into a finite-size scaling Ansatz produces more robust scaling behavior. In the present study we test the potential of such a procedure, in particular, to obtain the stiffness exponent for the hierarchical Migdal-Kadanoff lattice. Critical exponents for this model are known with great accuracy and any simulations can be executed to very large lattice sizes at almost any bond density, effecting a insightful comparison that highlights the advantages -- as well as the weaknesses -- of this method. These insights are applied to the Edwards-Anderson model in $d=3$ with Gaussian bonds.Comment: corrected version, 10 pages, RevTex4, 12 ps-figures included; related papers available a http://www.physics.emory.edu/faculty/boettcher

Detecting z > 2 Type IIn Supernovae

Type IIn supernovae (SNe IIn) dominate the brightest supernova events in observed FUV flux (~1200-2000A). We show that multi-band, multi-epoch optical surveys complete to m_r = 27 can detect the FUV emission of ~25 z > 2 SNe IIn deg^-2 yr^-1 rest-frame (~10 SNe IIn deg^-2 yr^-1 observed-frame) to 4 sigma using a technique monitoring color-selected galaxies. Moreover, the strength and evolution of the bright emission lines observed in low redshift SNe IIn imply that the Ly-a emission features in ~70% of z > 2 SNe IIn are above 8m-class telescope spectroscopic thresholds for ~2 yr rest-frame. As a result, existing facilities have the capability to both photometrically detect and spectroscopically confirm z > 2 SNe IIn and pave the way for efficient searches by future 8m-class survey and 30m-class telescopes. The method presented here uses the sensitivities and wide-field capabilities of current optical instruments and exploits (1) the efficiency of z > 2 galaxy color-selection techniques, (2) the intrinsic brightness distribution ( = -19.0 +/-0.9) and blue profile of SNe IIn continua, (3) the presence of extremely bright, long-lived emission features, and (4) the potential to detect blueshifted SNe Ly-a emission shortward of host galaxy Ly-a features.Comment: 26 pages (pre-print), 6 figures, accepted Ap

Recommendations for HER2 testing in the UK

Determining the HER2 status of breast carcinomas is a prerequisite for the use of the monoclonal antibody trastuzumab (Herceptin(R)), which has recently been licensed for the treatment of metastatic disease. This necessitates a test based on archival material. The preferred analyses are immunohistochemistry with fluorescent in situ hybridisation (FISH) as a follow up test for ambiguous results. Guidelines have been developed for standardised, well controlled procedures for the provision of reliable results. A group of three reference laboratories has been established to provide advice, quality assurance, and materials, where needed

Distance Oracles for Time-Dependent Networks

We present the first approximate distance oracle for sparse directed networks with time-dependent arc-travel-times determined by continuous, piecewise linear, positive functions possessing the FIFO property. Our approach precomputes $(1+\epsilon)-$approximate distance summaries from selected landmark vertices to all other vertices in the network. Our oracle uses subquadratic space and time preprocessing, and provides two sublinear-time query algorithms that deliver constant and $(1+\sigma)-$approximate shortest-travel-times, respectively, for arbitrary origin-destination pairs in the network, for any constant $\sigma > \epsilon$. Our oracle is based only on the sparsity of the network, along with two quite natural assumptions about travel-time functions which allow the smooth transition towards asymmetric and time-dependent distance metrics.Comment: A preliminary version appeared as Technical Report ECOMPASS-TR-025 of EU funded research project eCOMPASS (http://www.ecompass-project.eu/). An extended abstract also appeared in the 41st International Colloquium on Automata, Languages, and Programming (ICALP 2014, track-A

Interplay between attenuation- and virulence-factors of Babesia Bovis and their contribution to the establishment of persistent infections in cattle

Bovine babesiosis is an acute and persistent tick-borne global disease caused mainly by the intraerythrocytic apicomplexan parasites Babesia bovis and B. bigemina. B. bovis infected erythrocytes sequester in blood capillaries of the host (cytoadhesion), causing malaria-like neurological signs. Cytoadhesion and antigenic variation in B. bovis are linked to the expression of members of the Variant Erythrocyte Surface Antigen (VESA) gene family. Animals that survive acute B. bovis infection and those vaccinated with attenuated strains remain persistently infected, suggesting that B. bovis parasites use immune escape mechanisms. However, attenuated B. bovis parasites do not cause neurological signs in vaccinated animals, indicating that virulence or attenuation factors play roles in modulating parasite virulence phenotypes. Artificial overexpression of the SBP2t11 protein, a defined attenuation factor, was associated with reduced cytoadhesion, suggesting a role for this protein as a key modulator of virulence in the parasite. Hereby, we propose a model that might be functional in the modulation of B. bovis virulence and persistence that relies on the interplay among SBP2t, VESA proteins, cytoadhesion, and the immune responses of the host. Elucidation of mechanisms used by the parasite to establish persistent infection will likely contribute to the design of new methods for the control of bovine babesiosis