The inner radio jet region and the complex environment of SS433

We present multi-frequency VLBA+VLA observations of SS433 at 1.6, 5 and 15 GHz. These observations provide the highest angular resolution radio spectral index maps ever made for this object. Motion of the components of SS433 during the observation is detected. In addition to the usual VLBI jet structure, we detect two radio components in the system at an anomalous position angle. These newly discovered radio emitting regions might be related to a wind-like equatorial outflow or to an extension of the accretion disk. We show that the radio core component is bifurcated with a clear gap between the eastern and western wings of emission. Modelfitting of the precessing jets and the moving knots of SS433 shows that the kinematic centre -- i.e. the binary -- is in the gap between the western and eastern radio core components. Spectral properties and observed core position shifts suggest that we see a combined effect of synchrotron self-absorption and external free-free absorption in the innermost AU-scale region of the source. The spatial distribution of the ionized matter is probably not spherically symmetric around the binary, but could be disk-like.Comment: Accepted for publication by Astronomy and Astrophysic

Kinematics of parsec-scale structures in AGN: the 2cm VLBA Survey

We are investigating the kinematics of jets in active galactic nuclei on parsec scales by studying a representative population of sources. This study is being carried out using the Very Long Baseline Array at 15 GHz, with more than 800 images taken since 1994. In this contribution we present an overview of the diversity of kinematics for a complete sample of sources.Comment: Proceedings of the 6th European VLBI Network Symposium, Ros E., Porcas R.W., Lobanov, A.P., & Zensus, J.A. (eds), MPIfR, Bonn, Germany. 2 pages, 3 figures, needs evn2002.cls style fil

Variability and Velocity of Superluminal Sources

We investigate the relation between the Doppler factor determined from variations in total flux at 22 and 37 GHz, and the apparent transverse velocity determined from VLBA observations at 2 cm. The data are consistent with the relativistic beaming theory for compact radio sources, in that the distribution of beta_{app}/delta_{var}, for 30 quasars, is roughly consistent with a Monte Carlo simulation. The intrinsic temperature appears to be ~2x10^{10} K, close to the "equipartition value" calculated by Readhead (1994). We deduce the distribution of Lorentz factors for a group of 48 sources; the values range up to about gamma=40.Comment: To be published in "Radio Astronomy at the Fringe", ASP Conf. Ser. Vol. 300, J. A. Zensus, M. H. Cohen, & E. Ros (eds.), 8 pages, 3 figures, needs rafringe.st

Molecular footprint of drug-selective pressure in a human immunodeficiency virus transmission chain

Known human immunodeficiency virus (HIV) transmission histories are invaluable models for investigating the evolutionary and transmission dynamics of the virus and to assess the accuracy of phylogenetic reconstructions. Here we have characterized an HIV-1 transmission chain consisting of nine infected patients, almost all of whom were treated with antiviral drugs at later stages of infection. Partial pol and env gp41 regions of the HIV genome were directly sequenced from plasma viral RNA for at least one sample from each patient. Phylogenetic analyses in pol using likelihood methods inferred an evolutionary history not fully compatible with the known transmission history. This could be attributed to parallel evolution of drug resistance mutations resulting in the incorrect clustering of multidrug-resistant virus. On the other hand, a fully compatible phylogenetic tree was reconstructed from the env sequences. We were able to identify and quantify the molecular footprint of drug-selective pressure in pol using maximum likelihood inference under different codon substitution models. An increased fixation rate of mutations in the HIV population of the multidrug-resistant patient was demonstrated using molecular clock modeling. We show that molecular evolutionary analyses, guided by a known transmission history, can reveal the presence of confounding factors like natural selection and caution should be taken when accurate descriptions of HIV evolution are required.status: publishe

Activation of the E-cadherin/catenin complex in human MCF-7 breast cancer cells by all-trans-retinoic acid.

All-trans-retinoic acid (RA), like insulin-like growth factor I (IGF-I) and tamoxifen, inhibit invasion of human MCF-7/6 mammary cancer cells in vitro. For tamoxifen and for IGF-I, activation of the invasion-suppressor function of the E-cadherin/catenin complex was shown to be the most probable mechanism of the anti-invasive action. We did a series of experiments to determine whether the anti-invasive effect of RA also implicated the invasion-suppressor E-cadherin/catenin complex. Human MCF-7/6 mammary and HCT-8/R1 colon cancer cells, both with a dysfunctional E-cadherin/catenin complex, were treated with RA and the function of the complex was evaluated through Ca(2+)-dependent fast aggregation. Fast aggregation of both MCF-7/6 and HCT-8/R1 cells was induced by 1 microM RA. This effect was abolished by antibodies against E-cadherin. RA-induced fast aggregation was not sensitive to cycloheximide, tyrosine kinase inhibitors or antibodies against IGF-I or against the IGF-I receptor. RA did not stimulate IGF-I receptor phosphorylation or alter the E-cadherin/catenin complex, as evidenced by immunoprecipitation. RA up-regulates the function of the invasion-suppressor complex E-cadherin/catenin. Its action mechanism is different from that of IGF-I. RA may act as an anti-invasive agent with unique mechanisms of action

Doppler Boosting, Superluminal Motion, and the Kinematics of AGN Jets

We discuss results from a decade long program to study the fine-scale structure and the kinematics of relativistic AGN jets with the aim of better understanding the acceleration and collimation of the relativistic plasma forming AGN jets. From the observed distribution of brightness temperature, apparent velocity, flux density, time variability, and apparent luminosity, the intrinsic properties of the jets including Lorentz factor, luminosity, orientation, and brightness temperature are discussed. Special attention is given to the jet in M87, which has been studied over a wide range of wavelengths and which, due to its proximity, is observed with excellent spatial resolution. Most radio jets appear quite linear, but we also observe curved non-linear jets and non-radial motions. Sometimes, different features in a given jet appear to follow the same curved path but there is evidence for ballistic trajectories as well. The data are best fit with a distribution of Lorentz factors extending up to gamma ~30 and intrinsic luminosity up to ~10^26 W/Hz. In general, gamma-ray quasars may have somewhat larger Lorentz factors than non gamma-ray quasars. Initially the observed brightness temperature near the base of the jet extend up to ~5x10^13 K which is well in excess of the inverse Compton limit and corresponds to a large excess of particle energy over magnetic energy. However, more typically, the observed brightness temperatures are ~2x10^11 K, i.e., closer to equipartition.Comment: 10 pages, 12 color figures; proceedings of the 5th Stromlo Symposium: Disks, Winds, and Jets - from Planets to Quasars; accepted in Astrophysics & Space Scienc

E-QED: Electrical Bug Localization During Post-Silicon Validation Enabled by Quick Error Detection and Formal Methods

During post-silicon validation, manufactured integrated circuits are extensively tested in actual system environments to detect design bugs. Bug localization involves identification of a bug trace (a sequence of inputs that activates and detects the bug) and a hardware design block where the bug is located. Existing bug localization practices during post-silicon validation are mostly manual and ad hoc, and, hence, extremely expensive and time consuming. This is particularly true for subtle electrical bugs caused by unexpected interactions between a design and its electrical state. We present E-QED, a new approach that automatically localizes electrical bugs during post-silicon validation. Our results on the OpenSPARC T2, an open-source 500-million-transistor multicore chip design, demonstrate the effectiveness and practicality of E-QED: starting with a failed post-silicon test, in a few hours (9 hours on average) we can automatically narrow the location of the bug to (the fan-in logic cone of) a handful of candidate flip-flops (18 flip-flops on average for a design with ~ 1 Million flip-flops) and also obtain the corresponding bug trace. The area impact of E-QED is ~2.5%. In contrast, deter-mining this same information might take weeks (or even months) of mostly manual work using traditional approaches