Electrochemical behaviour of cytochrome c at low potentials

d.c. polarograms of bovine heart cytochrome c show reduction currents at low potentials. This is observed in buffer solutions with pH values between 1 and 10.5. These currents are attributed to catalytic hydrogen formation (pre-sodium currents). After succinylation of the protein, the current in glycine-NaOH buffer of pH 10.5 disappears almost completely, whereas that in acetate buffer of pH 4.5 is affected only slightly. It is concluded that different groups are responsible for the currents observed in these two buffer

The GROUSE project III: Ks-band observations of the thermal emission from WASP-33b

In recent years, day-side emission from about a dozen hot Jupiters has been detected through ground-based secondary eclipse observations in the near-infrared. These near-infrared observations are vital for determining the energy budgets of hot Jupiters, since they probe the planet's spectral energy distribution near its peak. The aim of this work is to measure the Ks-band secondary eclipse depth of WASP-33b, the first planet discovered to transit an A-type star. This planet receives the highest level of irradiation of all transiting planets discovered to date. Furthermore, its host-star shows pulsations and is classified as a low-amplitude delta-Scuti. As part of our GROUnd-based Secondary Eclipse (GROUSE) project we have obtained observations of two separate secondary eclipses of WASP-33b in the Ks-band using the LIRIS instrument on the William Herschel Telescope (WHT). The telescope was significantly defocused to avoid saturation of the detector for this bright star (K~7.5). To increase the stability and the cadence of the observations, they were performed in staring mode. We collected a total of 5100 and 6900 frames for the first and the second night respectively, both with an average cadence of 3.3 seconds. On the second night the eclipse is detected at the 12-sigma level, with a measured eclipse depth of 0.244+0.027-0.020 %. This eclipse depth corresponds to a brightness temperature of 3270+115-160 K. The measured brightness temperature on the second night is consistent with the expected equilibrium temperature for a planet with a very low albedo and a rapid re-radiation of the absorbed stellar light. For the other night the short out-of-eclipse baseline prevents good corrections for the stellar pulsations and systematic effects, which makes this dataset unreliable for eclipse depth measurements. This demonstrates the need of getting a sufficient out-of-eclipse baseline.Comment: 12 pages, 10 figures. Accepted for publication in Astronomy and Astrophysic

Detection of water absorption in the day side atmosphere of HD 189733 b using ground-based high-resolution spectroscopy at 3.2 microns

We report a 4.8 sigma detection of water absorption features in the day side spectrum of the hot Jupiter HD 189733 b. We used high-resolution (R~100,000) spectra taken at 3.2 microns with CRIRES on the VLT to trace the radial-velocity shift of the water features in the planet's day side atmosphere during 5 h of its 2.2 d orbit as it approached secondary eclipse. Despite considerable telluric contamination in this wavelength regime, we detect the signal within our uncertainties at the expected combination of systemic velocity (Vsys=-3 +5-6 km/s) and planet orbital velocity (Kp=154 +14-10 km/s), and determine a H2O line contrast ratio of (1.3+/-0.2)x10^-3 with respect to the stellar continuum. We find no evidence of significant absorption or emission from other carbon-bearing molecules, such as methane, although we do note a marginal increase in the significance of our detection to 5.1 sigma with the inclusion of carbon dioxide in our template spectrum. This result demonstrates that ground-based, high-resolution spectroscopy is suited to finding not just simple molecules like CO, but also to more complex molecules like H2O even in highly telluric contaminated regions of the Earth's transmission spectrum. It is a powerful tool that can be used for conducting an immediate census of the carbon- and oxygen-bearing molecules in the atmospheres of giant planets, and will potentially allow the formation and migration history of these planets to be constrained by the measurement of their atmospheric C/O ratios.Comment: 5 pages, 4 figures, accepted for publication in MNRAS Letter

Multidepot distribution planning at logistics service provider Nabuurs B.V.

Distribution networks of many logistics service providers have evolved from single-depot to complex, dynamic multidepot networks. In a single-depot network, the deliveries from each depot are planned for that depot only, and drivers return to the starting depot to pick up each new order. In a multidepot network, the deliveries from multiple depots can be planned simultaneously; therefore, a logistics service provider can efficiently combine its resources, thus reducing its labor and transport costs. However, an increasing emphasis on reliability, customization, and flexibility is affecting the logistics structures. This paper describes the shift from single-depot planning to multidepot planning for Nabuurs B.V., a large Benelux logistics service provider that implemented a centralized, automated multidepot planning process throughout its organization. We developed a simulation model to evaluate system performance and to address performance challenges. In this paper, we discuss the results of extensive simulation tests and the specific recommendations that Nabuurs B.V. management implemented

Titan's atmosphere as observed by Cassini/VIMS solar occultations: CH4_4, CO and evidence for C2_2H6_6 absorption

We present an analysis of the VIMS solar occultations dataset, which allows us to extract vertically resolved information on the characteristics of Titan's atmosphere between 100-700 km with a characteristic vertical resolution of 10 km. After a series of data treatment procedures, 4 occultations out of 10 are retained. This sample covers different seasons and latitudes of Titan. The transmittances show clearly the evolution of the haze and detect the detached layer at 310 km in Sept. 2011 at mid-northern latitudes. Through the inversion of the transmission spectra with a line-by-line radiative transfer code we retrieve the vertical distribution of CH$_4$ and CO mixing ratio. The two methane bands at 1.4 and 1.7 {\mu}m are always in good agreement and yield an average stratospheric abundance of $1.28\pm0.08$%. This is significantly less than the value of 1.48% obtained by the GCMS/Huygens instrument. The analysis of the residual spectra after the inversion shows that there are additional absorptions which affect a great part of the VIMS wavelength range. We attribute many of these additional bands to gaseous ethane, whose near-infrared spectrum is not well modeled yet. Ethane contributes significantly to the strong absorption between 3.2-3.5 {\mu}m that was previously attributed only to C-H stretching bands from aerosols. Ethane bands may affect the surface windows too, especially at 2.7 {\mu}m. Other residual bands are generated by stretching modes of C-H, C-C and C-N bonds. In addition to the C-H stretch from aliphatic hydrocarbons at 3.4 {\mu}m, we detect a strong and narrow absorption at 3.28 {\mu}m which we tentatively attribute to the presence of PAHs in the stratosphere. C-C and C-N stretching bands are possibly present between 4.3-4.5 {\mu}m. Finally, we obtain the CO mixing ratio between 70-170 km. The average result of $46\pm16$ ppm is in good agreement with previous studies.Comment: 51 pages, 28 figure