588 research outputs found
Transposition and duplication of MADS-domain transcription factor genes in annual and perennial Arabis species modulates flowering.
Novel use of plga microspheres to create an animal model of glaucoma with progressive neuroretinal degeneration
Progressive degeneration of neuroretinal tissue with maintained elevated intraocular pressure (IOP) to simulate chronic glaucoma was produced by intracameral injections of poly (lactic-co-glycolic) acid (PLGA) microspheres (Ms) in rat eyes. The right eye of 39 rats received different sizes of PLGA-Ms (2 µL suspension; 10% w/v): 14 with 38–20 µm Ms (Ms38/20 model) and 25 with 20–10 µm particles (Ms20/10 model). This novel glaucoma animal model was compared to the episcleral vein sclerosis (EPI) model (25 eyes). Injections were performed at baseline, two, four and six weeks. Clinical signs, IOP, retina and optic nerve thicknesses (using in vivo optical coherence tomography; OCT), and histological studies were performed. An IOP increment was observed in all three groups, however, the values obtained from the PLGA-Ms injection resulted lower with a better preservation of the ocular surface. In fact, the injection of Ms20/10 created a gentler, more progressive, and more sustained increase in IOP. This IOP alteration was correlated with a significant decrease in most OCT parameters and in histological ganglion-cell count for the three conditions throughout the eight-week follow-up. In all cases, progressive degeneration of the retina, retinal ganglion cells and optic nerve, simulating chronic glaucoma, was detected by OCT and corroborated by histological study. Results showed an alternative glaucoma model to the well-known episcleral vein model, which was simpler to perform, more reproducible and easier to monitor in vivo
Influence of chronic ocular hypertension on emmetropia: Refractive, structural and functional study in two rat models
Chronic ocular hypertension (OHT) influences on refraction in youth and causes glaucoma in adulthood. However, the origin of the responsible mechanism is unclear. This study analyzes the effect of mild-moderate chronic OHT on refraction and neuroretina (structure and function) in young-adult Long-Evans rats using optical coherence tomography and electroretinography over 24 weeks. Data from 260 eyes were retrospectively analyzed in two cohorts: an ocular normotension (ONT) cohort (20 mmHg), in which OHT was induced either by sclerosing the episcleral veins (ES group) or by injecting microspheres into the anterior chamber. A trend toward emmetropia was found in both cohorts over time, though it was more pronounced in the OHT cohort (p < 0.001), especially in the ES group (p = 0.001) and males. IOP and refraction were negatively correlated at week 24 (p = 0.010). The OHT cohort showed early thickening in outer retinal sectors (p < 0.050) and the retinal nerve fiber layer, which later thinned. Electroretinography demonstrated early supranormal amplitudes and faster latencies that later declined. Chronic OHT accelerates emmetropia in Long–Evans rat eyes towards slowly progressive myopia, with an initial increase in structure and function that reversed over time. © 2021 by the authors. Licensee MDPI, Basel, Switzerland
HADES RV Programme with HARPS-N at TNG VI. GJ 3942 b behind dominant activity signals
Short- to mid-term magnetic phenomena on the stellar surface of M-type stars
cannot only resemble the effects of planets in radial velocity data, but also
may hide them. We analyze 145 spectroscopic HARPS-N observations of GJ 3942
taken over the past five years and additional photometry to disentangle stellar
activity effects from genuine Doppler signals as a result of the orbital motion
of the star around the common barycenter with its planet. To achieve this, we
use the common methods of pre-whitening, and treat the correlated red noise by
a first-order moving average term and by Gaussian-process regression following
an MCMC analysis. We identify the rotational period of the star at 16.3 days
and discover a new super-Earth, GJ 3942 b, with an orbital period of 6.9 days
and a minimum mass of 7.1 Me. An additional signal in the periodogram of the
residuals is present but we cannot claim it to be related to a second planet
with sufficient significance at this point. If confirmed, such planet candidate
would have a minimum mass of 6.3 Me and a period of 10.4 days, which might
indicate a 3:2 mean-motion resonance with the inner planet
The HADES RV Programme with HARPS-N@TNG II. Data treatment and simulations
The distribution of exoplanets around low-mass stars is still not well
understood. Such stars, however, present an excellent opportunity of reaching
down to the rocky and habitable planet domains. The number of current
detections used for statistical purposes is still quite modest and different
surveys, using both photometry and precise radial velocities, are searching for
planets around M dwarfs. Our HARPS-N red dwarf exoplanet survey is aimed at the
detection of new planets around a sample of 78 selected stars, together with
the subsequent characterization of their activity properties. Here we
investigate the survey performance and strategy. From 2700 observed spectra, we
compare the radial velocity determinations of the HARPS-N DRS pipeline and the
HARPS-TERRA code, we calculate the mean activity jitter level, we evaluate the
planet detection expectations, and we address the general question of how to
define the strategy of spectroscopic surveys in order to be most efficient in
the detection of planets. We find that the HARPS-TERRA radial velocities show
less scatter and we calculate a mean activity jitter of 2.3 m/s for our sample.
For a general radial velocity survey with limited observing time, the number of
observations per star is key for the detection efficiency. In the case of an
early M-type target sample, we conclude that approximately 50 observations per
star with exposure times of 900 s and precisions of about 1 m/s maximizes the
number of planet detections
The AMS-RICH velocity and charge reconstruction
The AMS detector, to be installed on the International Space Station,
includes a Ring Imaging Cerenkov detector with two different radiators, silica
aerogel (n=1.05) and sodium fluoride (n=1.334). This detector is designed to
provide very precise measurements of velocity and electric charge in a wide
range of cosmic nuclei energies and atomic numbers. The detector geometry, in
particular the presence of a reflector for acceptance purposes, leads to
complex Cerenkov patterns detected in a pixelized photomultiplier matrix. The
results of different reconstruction methods applied to test beam data as well
as to simulated samples are presented. To ensure nominal performances
throughout the flight, several detector parameters have to be carefully
monitored. The algorithms developed to fulfill these requirements are
presented. The velocity and charge measurements provided by the RICH detector
endow the AMS spectrometer with precise particle identification capabilities in
a wide energy range. The expected performances on light isotope separation are
discussed.Comment: Contribution to the ICRC07, Merida, Mexico (2007); Presenter: F.
Bara
The RICH detector of the AMS-02 experiment: status and physics prospects
The Alpha Magnetic Spectrometer (AMS), whose final version AMS-02 is to be
installed on the International Space Station (ISS) for at least 3 years, is a
detector designed to measure charged cosmic ray spectra with energies up to the
TeV region and with high energy photon detection capability up to a few hundred
GeV. It is equipped with several subsystems, one of which is a proximity
focusing RICH detector with a dual radiator (aerogel+NaF) that provides
reliable measurements for particle velocity and charge. The assembly and
testing of the AMS RICH is currently being finished and the full AMS detector
is expected to be ready by the end of 2008. The RICH detector of AMS-02 is
presented. Physics prospects are briefly discussed.Comment: 5 pages. Contribution to the 10th ICATPP Conference on Astroparticle,
Particle, Space Physics, Detectors and Medical Physics Applications (Como
2007). Presenter: Rui Pereir
Chronic glaucoma using biodegradable microspheres to induce intraocular pressure elevation. Six-month follow-up
Background: To compare two prolonged animal models of glaucoma over 24 weeks of follow-up. A novel pre-trabecular model of chronic glaucoma was achieved by injection of biodegradable poly lactic-co-glycolic acid (PLGA) microspheres (10–20 µm) (Ms20/10) into the ocular anterior chamber to progressively increase ocular hypertension (OHT). Methods: Rat right eyes were injected to induce OHT: 50% received a suspension of Ms20/10 in the anterior chamber at 0, 2, 4, 8, 12, 16 and 20 weeks, and the other 50% received a sclerosing episcleral vein injection biweekly (EPIm). Ophthalmological clinical signs, intraocular pressure (IOP), neuroretinal functionality measured by electroretinography (ERG), and structural analysis of the retina, retinal nerve fiber layer (RNFL) and ganglion cell layer (GCL) protocols using optical coherence tomography (OCT) and histological exams were performed. Results: Both models showed progressive neuroretinal degeneration (p < 0.05), and contralateral eye affectation. The Ms20/10 model showed a more progressive increase in IOP and better preservation of ocular surface. Although no statistical differences were found between models, the EPIm showed a tendency to produce thicker retinal and thinner GCL thicknesses, slower latency and smaller amplitude as measured using ERG, and more aggressive disturbances in retinal histology. In both models, while the GCL showed the greatest percentage loss of thickness, the RNFL showed the greatest and earliest rate of thickness loss. Conclusions: The intracameral model with biodegradable microspheres resulted more like the conditions observed in humans. It was obtained by a less-aggressive mechanism, which allows for adequate study of the pathology over longer periods. © 2021 by the authors. Licensee MDPI, Basel, Switzerland
New physics searches at near detectors of neutrino oscillation experiments
We systematically investigate the prospects of testing new physics with tau
sensitive near detectors at neutrino oscillation facilities. For neutrino beams
from pion decay, from the decay of radiative ions, as well as from the decays
of muons in a storage ring at a neutrino factory, we discuss which effective
operators can lead to new physics effects. Furthermore, we discuss the present
bounds on such operators set by other experimental data currently available.
For operators with two leptons and two quarks we present the first complete
analysis including all relevant operators simultaneously and performing a
Markov Chain Monte Carlo fit to the data. We find that these effects can induce
tau neutrino appearance probabilities as large as O(10^{-4}), which are within
reach of forthcoming experiments. We highlight to which kind of new physics a
tau sensitive near detector would be most sensitive.Comment: 20 pages, 2 figures, REVTeX
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