patients

Introduction: The percutaneous catheterization of various arteries is used in visualization of coronary arteries.Aim: We aimed to determine whether arterial blood samples withdrawn from femoral arteries during standard ludkin's technique in patients evaluated with coronary angiography can also be used to determine some biochemical parameters.Material and methods: In 50 controls (25 males and 25 females) and 73 coronary artery disease (CAD) patients (10 females and 63 males) paraoxonase-1 (PON1) activity, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C), high-density lipoprotein cholesterol (HDL-C) and triglyceride (TG) levels were measured using colorimetric methods. lipid peroxidation marker levels (conjugated dienes (CD) and thiobarbituric acid-reactive substances (TBARS)) were measured manually.Results: There was no difference in lipid and lipid peroxidation marker levels, PON1 activity and TC/HDL-C, LDL-C/HDL-C and PON1/HDL-C ratios between arterial and venous blood samples. LDL-C, CD and TBARS levels and TC/HDL-C and LDL-C/HDL-C ratios were significantly lower in both arterial and venous blood samples of controls compared with CAD patients. Paraoxonase-1 activity, HDL-C level and PON1/HDL-C ratio were higher in controls than CAD patients. On multiple logistic regression analysis, risk factors associated with CAD were found to be the levels of arterial CD, venous CD, arterial TBARS and arterial LDL-C/HDL-C ratios in CAD patients.Conclusions: Our study might indicate that arterial blood samples can also be used as well as venous samples to determine these parameters. On the other hand, elevated arterial lipid peroxides are associated with cardiovascular complications, presumably by decreasing PON1 activity

Lysophosphatidic Acid Modifies the Response of PC3 Prostate Cancer Cells to Chemotherapeutics

OBJECTIVE: Prostate cancer is the most frequently diagnosed cancer among men. Docetaxel, estramustine, and mitoxantrone are commonly used chemotherapy agents for the treatment of prostate cancer. However, lysophosphatidic acid (LPA), a biologically active glycerophospholipid derivative, induces proliferation and inhibits apoptosis in prostate cancer cells. The aim of this study was to investigate the effects of LPA against cell toxicity of docetaxel, estramustine and mitoxantrone. MATERIALS AND METHODS: Prostate carcinoma PC3 cells were separately treated with docetaxel, estramustine and mitoxantrone in combinations with LPA. BrdU incorporation assay was used to assess the cell proliferation. Besides, colony forming ability of cells were measured by staining with crystal violet. The ratio of apoptotic cells was also detected by flow cytometry. RESULTS: All the chemotherapeutic drugs decreased the proliferation and colony formation of PC3 cells whereas these parameters were found to be significantly increased in the cells treated with LPA alone. Treatment of drugs together with LPA increased cell proliferation and colony formation compared to the treatment of with drugs alone. Also, LPA was seen to modify the apoptotic effects of docetaxel, estramustine and mitoxantrone. CONCLUSIONS: Our results showed that LPA contributed to cell survival and proliferation in PC3 prostate cancer cells. LPA created a resistance against cell death induced by docetaxel, estramustine and mitoxantrone. Our study supports the idea that LPA or its signaling pathways may be a promising target for the treatment of prostate cancer and prevention of resistance to chemotherapy

Increased serum S-TRAIL level in newly diagnosed stage-IV lung adenocarcinoma but not squamous cell carcinoma is correlated with age and smoking

PubMedID: 24083751Background: Lung cancer is the leading cause of cancer mortality in the world. Many factors can protect against or facilitate its development. A TNF family member TRAIL, has a complex physiological role beyond that of merely activating the apoptotic pathway in cancer cells. Vitamin D is converted to its active form locally in the lung, and is also thought to play an important role in lung health. Our goal was to investigate the possible clinical significance of serum sTRAIL and 1,25-dihydroxyvitamin D(3) levels in patients with non-small cell lung cancer (NSCLC). Materials and Methods: Totals of 18 consecutive adenocarcinoma and 22 squamous cell carcinoma patients with stage-IV non-small cell lung cancer referred to our institute were included in this study. There were 12 men and 6 women, with ages ranging from 38 to 97 (mean 60.5) years with adenocarcinoma, and 20 men and 2 women, with ages ranging from 46 to 80 (mean 65) years with squamous cell carcinoma. Serum levels of sTRAIL and 1,25-dihydroxyvitamin D(3) were measured in all samples at the time of diagnosis. Results: sTRAIL levels in NSCLC patients were higher than in the control group. Although there was no correlation between patient survival and sTRAIL levels, the highest sTRAIL levels were correlated with age and cigarette smoking in the adenocarcinoma patients. sTRAIL level in healthy individuals were correlated with serum 1,25-dihydroxyvitamin D(3). Conclusions: Serum sTRAIL concentrations were increased in NSCLC patients, and correlated with age and smoking history, but not with overall survival

Effects of chronic administration of adipokinetic and hypertrehalosemic hormone on animal behavior, BDNF, and CREB expression in the hippocampus and neurogenesis in mice

PMID = 2679199

Effects of Asenapine and Paliperidone on Depression, Anxiety and Analgesy in Mice: Alterations in Brain Neurotrophic Factors, Neurogenesis, and Blood Enzyme Levels

PMID = 3038440

Effect of sulfite on red blood cell deformability ex vivo and in normal and sulfite oxidase-deficient rats in vivo

The effect of sulfite, a widely used food additive, on red blood cell deformability ex vivo and in vivo was investigated. Ex vivo experiments were conducted in human blood exposed to sulfite (5, 10 and 20 mM). In vivo experiments were carried out in sulfite oxidase-competent (SOXC) and sulfite oxidase-deficient (SOXD) rats. In the in vivo experiments, sulfite was administered in the form of sodium metabisulfite (Na2S 2O5, 25 mg/kg/day) via drinking water. Vitamin E dissolved in olive oil at a dose of 50 mg/kg was administered by gastric gavages. Red blood cell (RBC) deformability was determined at various fluid shear stresses using an ektacytometer. Ex vivo sulfite exposure to RBC did not affect RBC deformability. In the in vivo experiments, although RBC deformability was not affected by sulfite treatment in SOXD rats, it was found to be significantly increased in SOXC rats. Vitamin E treatment in combination with sulfite caused impairment in RBC deformability in both SOXC and SOXD rats. We suggest that sulfite needs to be oxidized in order to improve RBC deformability. © Springer-Verlag 2005