Bis(thiosemicarbazones) as bifunctional chelators for the room temperature 64-copper labeling of peptides.

A range of new carboxylate functionalised bis(thiosemicarbazone) ligands and their Cu(II) complexes have been prepared, fully characterised and radiolabeled in high yield with both (64)Cu and (99m)Tc. Conjugation to a bombesin derivative was achieved using standard solid phase synthetic methodologies and the (64)Cu-labeled conjugate was shown to have good tumour uptake in mice with xenografted PC-3 tumours

Using Style to Understand Descriptions of Software Architecture

The software architecture of most systems is described informally and diagrammatically. In order for these descriptions to be meaningful at all, figures are understood by interpreting the boxes and lines in specific, conventionalized ways [5]. The imprecision of these interpretations has a number of limitations. In this paper we consider these conventionalized interpretations as architectural styles and provide a formal framework for their uniform definition. In addition to providing a template for precisely defining new architectural styles, this framework allows for the proof that the notational constraints on a style are sufficient to guarantee the meanings of all described systems and provides a unified semantic base through which different stylistic interpretations can be compared

Copy number abnormalities analysis of CD138⁺⁺ and CD138^low cells.

<p>Log ratio plot of all chromosomes corresponding to CD138⁺⁺ RPMI-8266 cells (n = 3; left panel) and CD138^low RPMI-8266 cells (n = 3; right panel) on the basis of Cytoscan HD array generated with Nexus.</p

Proliferation of CD138⁺⁺ and CD138^low subpopulations.

<p>(A) Left: Cell percentage of CD138⁺⁺ and CD138^low RPMI-8226 or NCI-H929 cells in G0-G1, S and G2-M phases. The results are expressed as the means ± SEM of at least three independent experiments. Right: Representative DRAQ5 histograms for each indicated population. (B) Left: Relative Ki-67 MFI of CD138⁺⁺ and CD138^low RPMI-8226 and NCI-H929 cells with respect to isotype control. Results are expressed as the means ± SEM of three independent experiments. Right: Representative Ki-67 histograms for each indicated population. Filled histograms (isotype control); open histograms (Ki-67).</p