Global Epidemiology of Tuberculosis: Past, Present and Future

No9 abstract

Prevalence of Smear Positive Pulmonary Tuberculosis and its Associated Risk Factors among Patients Attending Nekemte Referral Hospital, Western Ethiopia

Tuberculosis (TB), one of the widest spread infectious diseases, is the leading cause of death in the world. TB is one of the major causes of morbidity and mortality in the horn of Africa with Ethiopia carrying a heavy burden; it is the third most populous country in Africa. The aim of this study is to determine the prevalence of smear positive pulmonary TB (PTB) and its associated risk factors among TB suspected patients attending Nekemte Referral Hospital. Oromia Region State, Western,  Ethiopia. A cross sectional study was conducted among 170 TB suspected patients attending Nekemte Referral Hospital from April 1 to May 15, 2012. Convenience sampling technique was used to select the study subjects. The sputum sample was collected and examined following national guide line for diagnosis of TB using direct Ziehl – Nelson staining and sodium hypochlorite concentration techniques. To collect data related to associated risk factors for PTB, pretest structured question was employed. The collected data was analyzed using SPSS version 16 and described using descriptive statistic and binary logistic regression modeling. The prevalence of smear-positive PTB is 9.41% and 15.88% by direct and sodium hypochlorite concentration Ziehle-Nelson staining technique, respectively. Factors significantly associated with smear-positive PTB were level ofeducation primary (AOR=17.78) and secondary education (AOR=736.19), occupation of merchant (AOR =0.03), sex male (AOR= 0.089), marital status of married (AOR=0.26), monthly income between 500-1000 (OR 0.002), urban residence (AOR= 0.39) and having close contact history with TB patient (OR=AO.113). The prevalence of Smear positive PTB is high at the study area and it is mostly associated with soci-demographic factorsand close contact with TB patient

Inheritance of Resistance to Striga in Sorghum Genotype SRN39

Striga hermonthica (Del.) Benth., a parasitic weed of grasses, causes major yield reductions in the principal cereal crops of semi-arid Africa. Cultivar resistance is the most economic control measure, since adapted, resistant cultivars can be grown without additional input from the subsistence farmer. Information on the genetics of resistance to S. hermonthica is scant. This is partially attributable to the rarity of germplasms which exhibit stable resistance across geographical regions. The objective of this study was to determine if the stable resistance observed in sorghum [Sorghum bicolor (L.) Moench] cultivar SRN39 is heritable. Crosses were made between SRN39 and a susceptible parent, P954063. Parental, F1, F2 and backcross generations were grown in infested pots and development of both host and parasite was monitored. Significant variation among genotypes was observed for both host traits and effects on parasite populations. The F1 did not differ significantly in Striga resistance from the susceptible parent, suggesting recessive inheritance. However, hybrid vigor was exhibited by the F1 which yielded and developed as well as the resistant parent. Broad sense heritability ranged from 0.23 to 0.55 for host traits and from 0.10 to 0.43 for effect of genotypes on the Striga population. Joint scaling tests showed that observed variation in each host or parasite trait consisted of additive and dominance components, suggesting possible progress could be made with appropriate selection scheme

Research into Germination of Striga Seed by Sorghum Root Exudates

This research was undertaken to develop a laboratory assay to permit screening of sorghum lines for low production of sorgoleone, the first germination stimulant of Striga seed to be isolated from host root exudate. Our findings led us to assay for the other substances in sorghum root exudate which control germination of Striga seed. A convenient petri disch assay, described elsewhere, permits host selection for low stimulation of germination. Work to isolate, assay and characterize these compounds is underway at Purdue Universit

Sorghum Brown Midrib19 (Bmr19) Gene Links Lignin Biosynthesis to Folate Metabolism

Genetic analysis of brown midrib sorghum (Sorghum bicolor) mutant lines assembled in our program has previously shown that the mutations fall into four allelic groups, bmr2, bmr6, bmr12 or bmr19. Causal genes for allelic groups bmr2, bmr6 and bmr12, have since been identified. In this report, we provide evidence for the nature of the bmr19 mutation. This was accomplished by introgressing each of the four bmr alleles into nine different genetic backgrounds. Polymorphisms from four resequenced bulks of sorghum introgression lines containing either mutation, relative to those of a resequenced bulk of the nine normal midrib recurrent parent lines, were used to locate their respective causal mutations. The analysis confirmed the previously reported causal mutations for bmr2 and bmr6 but failed in the case of bmr12-bulk due to a mixture of mutant alleles at the locus among members of that mutant bulk. In the bmr19-bulk, a common G → A mutation was found among all members in Sobic.001G535500. This gene encodes a putative folylpolyglutamate synthase with high homology to maize Bm4. The brown midrib phenotype co-segregated with this point mutation in two separate F2 populations. Furthermore, an additional variant allele at this locus obtained from a TILLING population also showed a brown midrib phenotype, confirming this locus as Bmr19

The Occurrence of Uterine Benign Diseases and their Histomorphologic Characters

Uterine diseases are several and can develop from any part of the organ. Noticeable benign diseases are type called hydatidiform mole considered benign among gestational trophoblastic disease and is said to occur from abnormal fertilization of abnormal ova. Other forms are inflammatory, proliferative of insidious cell/tissue implant, hormonal induced, and infectious in their origin. Samples were obtained from patients consulted and admitted into Department of Morbid Anatomy of Ebonyi State University Teaching Hospital. Clinical and histopathological record books were reviewed alongside processed tissues, and slides stained by popular heamatoxylin and eosin technique in the 3 year study period. Of the 642 gynecological samples searched, result showed a progressive increase in number as the years empty from 2011 to 2013 presenting 8 uterine diseases in a population of 116 cases. A total of 518 cases were product of conception of which 250 were as a result of incomplete spontaneous abortions and 200 inappropriate criminal abortions, while 68 could not be associated with any definite cause. Leiomyoma cases were 75%, uterine/vaginal prolapsed were 6%, molar pregnancies and endometriosis 5% and while endometrial hyperplasia and uterine atrophy were 3% each and 2% each were for uterine polyp and adenomyosis in all of the 116 cases. Six age groups were involved showing that 7 diseases and total frequency of 50% occurred with age group (40-49) and is seconded by (30-39) which had 5 conditions with frequency of 21%. Attempted provisional diagnosis was based on clinical presentations, and of 116 cases 80% were confirmed accurate by laboratory diagnosis. Clinical characters of leiomyomas were the same while histomorphologic features were not entirely consistently same in all

Exploring barriers to the use of formal maternal health services and priority areas for action in Sidama zone, southern Ethiopia.

In 2015 the maternal mortality ratio for Ethiopia was 353 per 100,000 live births. Large numbers of women do not use maternal health services. This study aimed to identify factors influencing the use of maternal health services at the primary health care unit (PHCU) level in rural communities in Sidama zone, south Ethiopia in order to design quality improvement interventions. We conducted a qualitative study in six woredas in 2013: 14 focus group discussions (FGDs) and 44 in-depth interviews with purposefully selected community members (women, male, traditional birth attendants, local kebele administrators), health professionals and health extension workers (HEWs) at PHCUs. We digitally recorded, transcribed and thematically analysed the interviews and FGDs using Nvivo. The 'three delay model' informed the analytical process and discussion of barriers to the use of maternal health services. Lack of knowledge on danger signs and benefits of maternal health services; cultural and traditional beliefs; trust in TBAs; lack of decision making power of women, previous negative experiences with health facilities; fear of going to an unfamiliar setting; lack of privacy and perceived costs of maternal health services were the main factors causing the first delay in deciding to seek care. Transport problems in inaccessible areas were the main contributing factor for the second delay on reaching care facilities. Lack of logistic supplies and equipment, insufficient knowledge and skills and unprofessional behaviour of health workers were key factors for the third delay in accessing quality care. Use of maternal health services at the PHCU level in Sidama zone is influenced by complex factors within the community and health system. PHCUs should continue to implement awareness creation activities to improve knowledge of the community on complications of pregnancy and benefits of maternal health services. The health system has to be responsive to community's cultural norms and practices. The mangers of the woreda health office and health centres should take into account the available budgets; work on ensuring the necessary logistics and supplies to be in place at PHCU

Technology generation to dissemination:lessons learned from the tef improvement project

Indigenous crops also known as orphan crops are key contributors to food security, which is becoming increasingly vulnerable with the current trend of population growth and climate change. They have the major advantage that they fit well into the general socio-economic and ecological context of developing world agriculture. However, most indigenous crops did not benefit from the Green Revolution, which dramatically increased the yield of major crops such as wheat and rice. Here, we describe the Tef Improvement Project, which employs both conventional- and molecular-breeding techniques to improve tef\u2014an orphan crop important to the food security in the Horn of Africa, a region of the world with recurring devastating famines. We have established an efficient pipeline to bring improved tef lines from the laboratory to the farmers of Ethiopia. Of critical importance to the long-term success of this project is the cooperation among participants in Ethiopia and Switzerland, including donors, policy makers, research institutions, and farmers. Together, European and African scientists have developed a pipeline using breeding and genomic tools to improve the orphan crop tef and bring new cultivars to the farmers in Ethiopia. We highlight a new variety, Tesfa, developed in this pipeline and possessing a novel and desirable combination of traits. Tesfa\u2019s recent approval for release illustrates the success of the project and marks a milestone as it is the first variety (of many in the pipeline) to be released

The GCP molecular marker toolkit, an instrument for use in breeding food security crops

Crop genetic resources carry variation useful for overcoming the challenges of modern agriculture. Molecular markers can facilitate the selection of agronomically important traits. The pervasiveness of genomics research has led to an overwhelming number of publications and databases, which are, nevertheless, scattered and hence often difficult for plant breeders to access, particularly those in developing countries. This situation separates them from developed countries, which have better endowed programs for developing varieties. To close this growing knowledge gap, we conducted an intensive literature review and consulted with more than 150 crop experts on the use of molecular markers in the breeding program of 19 food security crops. The result was a list of effectively used and highly reproducible sequence tagged site (STS), simple sequence repeat (SSR), single nucleotide polymorphism (SNP), and sequence characterized amplified region (SCAR) markers. However, only 12 food crops had molecular markers suitable for improvement. That is, marker-assisted selection is not yet used for Musa spp., coconut, lentils, millets, pigeonpea, sweet potato, and yam. For the other 12 crops, 214 molecular markers were found to be effectively used in association with 74 different traits. Results were compiled as the GCP Molecular Marker Toolkit, a free online tool that aims to promote the adoption of molecular approaches in breeding activities