Vegetation diversity on coal mine spoil heaps-how important is the texture of the soil substrate?

© 2019, The Author(s). The relationship between the size of the particle fractions of the soil substrate and the diversity of the spontaneously developing vegetation was investigated on coal mine spoil heaps in Upper Silesia (Southern Poland). The analyses were based on 2567 research plots of developed spontaneous vegetation and their associated soil substrate samples collected from 112 coal mine spoil heaps. For each research plot the prevailing particle size fraction was determined (stones, gravel, sand, silt), the species composition and abundance was recorded and the species richness (S), Shannon-Wiener diversity index (H′), Simpson (C) and Evenness (E) indices were used to determine species diversity. From a total of 119 research plots (in all particle size fraction categories), the values of 15 physicochemical properties (pH, electrical conductivity, water holding capacity, moisture, carbon content, total N, available P, Mg and exchange cations Ca, Mg, K, Na, fine particles (%), gravel (%), stone (%)) were obtained to asses their impact on the floristic composition of vegetation patches using Canonical Correspondence Analysis (CCA). Additionally, functional traits of the dominant species of each vegetation patch (life forms, life strategies and socio-ecological groups), were selected to analyse their relation to substrate texture. It was shown that the highest species richness and the highest values for Shannon-Wiener diversity index, as well as Simpson and Evenness indices, were obtained in plots formed on stones. Moreover, the greatest variation in the participation of species representing different habitats, life forms, and life strategies was found on gravelly substrates. Contrary to our expectations, the vegetation diversity (in terms of both species and their functional traits) was not highest in habitats with a high composition of fine size particles.Published versio

The role of plants and soil properties in the enzyme activities of substrates on hard coal mine spoil heaps

© 2021 The Authors. Published by Springer. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.1038/s41598-021-84673-0Knowledge about biotic (plant species diversity, biomass) and/or abiotic (physicochemical substrate parameters) factors that determine enzyme activity and functional diversity of the substrate on hard coal spoil heaps is limited. Spontaneously developed vegetation patches dominated by herbaceous species commonly occurring on these spoil heaps: grasses (Poa compressa, Calamagrostis epigejos) and forbs (Daucus carota, Tussilago farfara), were examined. The activity of dehydrogenase and alkaline phosphatase was twice as high in plots dominated by grass species compared with those dominated by forbs. Significant positive correlations were found between the activity of dehydrogenase and alkaline phosphatase with pH, available P, soil moisture, and water holding capacity and negative correlations between the activity of urease and soil organic carbon. Strong positive correlations were found between values for Shannon–Wiener diversity index, evenness, species richness and soil functional diversity in plots dominated by grasses. We found that the soil physicochemical parameters had a greater impact on enzyme activity of the substrate than plant biomass and species diversity. However, grasses, through their extensive root system, more effectively increased enzyme activity and health of the substrate than other herbaceous species, and as they stabilize the substrate and form dense plant cover, they can be recommended for reclamation purposes.This study was funded by the InfoRevita project (TANGO1/268600/ NCBR/2015) financed by The National Centre for Research and Development in Poland.Accepted versio

Diversity of vegetation dominated by selected grass species on coal-mine spoil heaps in terms of reclamation of post-industrial areas

Published by Polish Society of Ecological Engineering (PTIE). This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.12911/22998993/93870© 2018, Polish Society of Ecological Engineering (PTIE). Grasses have a considerable potential for the adaptation to various, often extreme, habitat conditions. The aim of the work was to present the vegetation diversity of the coal-mine spoil heaps with the dominant share of grasses and to identify the main factors responsible for this diversity in the aspect of post-industrial land reclamation. The communities differ in reference to the species preferences to light, moisture, soil fertility and reaction, which is reflected in the wide variety of microhabitats in the area. It was shown that the increase in the abundance of certain grass species, including Calamagrostis epigejos, Festuca rubra, Festuca arundinacea, Phragmites australis, has a significant negative impact on the species richness, species diversity and the uniformity of distribution of species of the plant community. Preliminary analyses revealed that on post-mining waste, the biomass production of the dominant species is negatively correlated with biodiversity. The knowledge about the biology and ecology of grass species, as well as on the assembly rules may be used in the reclamation of degraded areas. Gaining the knowledge about the vegetation diversity of the coal-mine spoil heaps with the dominant share of grasses can be useful in planning the reclamation works, taking into account natural processes, which leads to the creation of a permanent vegetation cover at a given site, protecting it against water or wind erosion. In the future these areas may provide a number of important ecosystem services.Published versio

Preliminary effects of fertilization on ecochemical soil condition in mature spruce stands experiencing dieback in the Beskid Śla̧ski and Żywiecki Mountains, Poland

In recent years, there has been the phenomena of spruce dieback in Europe. Significant areas of spruce low mortality now cover both sides of the Polish southern border. We evaluated ecochemical parameters influencing the heavy dieback occurring in mature spruce stands in the Polish Carpathian Mountains. Dolomite, magnesite and serpentinite fertilizers were applied to experimental plots located in 100-year-old stands in the autumn of 2008. The experimental plots were located in the mid-elevational forest zone (900-950 m) on two nappes of the flysch Carpathians: Magura (Ujsoły Forest District) and Silesian (Wisła Forest District). The saturation of the studied soils demonstrates moderate resilience of soils in Wisła Forest District in relation to acid load and high flexibility of the Ujsoły soils. After application of the fertilizers, an increase of Mg, Ca and Mb was noted in the soil solution, determined in the overlaying highly acidic organic horizons through the ion-exchange buffering mechanism of highly protonated functional groups with high buffering capacity. Magnesium concentration increased following fertilization, presenting a potential improvement of forest growth capacity without the hazard of adverse side effects of liming. Aluminium stress in old spruce is unlikely, while trees in the control plots in Wisła Forest District may already be sensitive to aluminium stress. Serpentinite fertilization improved the supply of soils in magnesium without causing significant changes in the pH of the soil. Such changes in the pH were found in dolomite and magnesite fertilizer. © The Author(s) 2014

Relationship Between Anti-DFS70 Autoantibodies and Oxidative Stress

Background: The anti-DFS70 autoantibodies are one of the most commonly and widely described agent of unknown clinical significance, frequently detected in healthy individuals. It is not known whether the DFS70 autoantibodies are protective or pathogenic. One of the factors suspected of inducing the formation of anti-DFS70 antibodies is increased oxidative stress. We evaluated the coexistence of anti-DFS70 antibodies with selected markers of oxidative stress and investigated whether these antibodies could be considered as indirect markers of oxidative stress. Methods: The intensity of oxidative stress was measured in all samples via indices of free-radical damage to lipids and proteins such as total oxidant status (TOS), concentrations of lipid hydroperoxides (LPH), lipofuscin (LPS), and malondialdehyde (MDA). The parameters of the non-enzymatic antioxidant system, such as total antioxidant status (TAS) and uric acid concentration (UA), were also measured, as well as the activity of superoxide dismutase (SOD). Based on TOS and TAS values, the oxidative stress index (OSI) was calculated. All samples were also tested with indirect immunofluorescence assay (IFA) and 357 samples were selected for direct monospecific anti DFS70 enzyme-linked immunosorbent assay (ELISA) testing. Results: The anti-DFS70 antibodies were confirmed by ELISA test in 21.29% of samples. Compared with anti-DFS70 negative samples we observed 23% lower concentration of LPH (P =.038) and 11% lower concentration of UA (P =.005). TOS was 20% lower (P =.014). The activity of SOD was up to 5% higher (P =.037). The Pearson correlation showed weak negative correlation for LPH, UA, and TOS and a weak positive correlation for SOD activity. Conclusion: In samples positive for the anti-DFS70 antibody a decreased level of oxidative stress was observed, especially in the case of samples with a high antibody titer. Anti-DFS70 antibodies can be considered as an indirect marker of reduced oxidative stress or a marker indicating the recent intensification of antioxidant processes

Biological invasions are a population-level rather than a species-level phenomenon.

Biological invasions pose a rapidly expanding threat to the persistence, functioning and service provisioning of ecosystems globally, and to socio-economic interests. The stages of successful invasions are driven by the same mechanism that underlies adaptive changes across species in general-via natural selection on intraspecific variation in traits that influence survival and reproductive performance (i.e., fitness). Surprisingly, however, the rapid progress in the field of invasion science has resulted in a predominance of species-level approaches (such as deny lists), often irrespective of natural selection theory, local adaptation and other population-level processes that govern successful invasions. To address these issues, we analyse non-native species dynamics at the population level by employing a database of European freshwater macroinvertebrate time series, to investigate spreading speed, abundance dynamics and impact assessments among populations. Our findings reveal substantial variability in spreading speed and abundance trends within and between macroinvertebrate species across biogeographic regions, indicating that levels of invasiveness and impact differ markedly. Discrepancies and inconsistencies among species-level risk screenings and real population-level data were also identified, highlighting the inherent challenges in accurately assessing population-level effects through species-level assessments. In recognition of the importance of population-level assessments, we urge a shift in invasive species management frameworks, which should account for the dynamics of different populations and their environmental context. Adopting an adaptive, region-specific and population-focused approach is imperative, considering the diverse ecological contexts and varying degrees of susceptibility. Such an approach could improve and refine risk assessments while promoting mechanistic understandings of risks and impacts, thereby enabling the development of more effective conservation and management strategies