In vitro propagation of Rosa hybrida L. cv. Al-Taif Rose plant

In this study, a protocol for in vitro propagation of Rosa hybrida L. cv. Al-Taif Rose was established using nodal segments harboring axillary buds as explants. In vitro stages of shoot initiation, multiplication and elongation were performed. Explants were cultured on solid Murashige and Skoog medium (MS) supplemented with different concentrations of benzyl aminopurine (BAP, 1, 2 and 3 mg/l) in combination with 1 mg/l kinetin (Kn). Effect of different concentrations and combinations of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) on root formation of shoots were studied. The highest percentage of shoot initiation (85%) was observed on MS medium containing 2 mg/l BAP + 1 mg/l Kn, whereas maximum average number of multiplied shoots (2.7) was produced on MS medium with 3 mg/l BAP + 1 mg/l Kn. Highest average number of elongated shoots (26.7) was noticed on MS medium containing 1 mg/l BAP and 1 mg/l Kn. For rooting, highest percentage (66.7%) of rooted shoots was obtained on MS medium supplemented with 2 mg/l IBA. Plantlets with 4 to 5 roots of 3 to 5 cm length were successfully transferred to pots containing sterile peat moss for acclimatization.Keywords: Rosa hybrid L. cv. Al-Taif Rose, axillary bud explants, in vitro propagation, multiplication, acclimatizatio

PyOED: An Extensible Suite for Data Assimilation and Model-Constrained Optimal Design of Experiments

This paper describes the first version (v1.0) of PyOED, a highly extensible scientific package that enables developing and testing model-constrained optimal experimental design (OED) for inverse problems. Specifically, PyOED aims to be a comprehensive Python toolkit for model-constrained OED. The package targets scientists and researchers interested in understanding the details of OED formulations and approaches. It is also meant to enable researchers to experiment with standard and innovative OED technologies with a wide range of test problems (e.g., simulation models). Thus, PyOED is continuously being expanded with a plethora of Bayesian inversion, DA, and OED methods as well as new scientific simulation models, observation error models, and observation operators. These pieces are added such that they can be permuted to enable testing OED methods in various settings of varying complexities. The PyOED core is completely written in Python and utilizes the inherent object-oriented capabilities; however, the current version of PyOED is meant to be extensible rather than scalable. Specifically, PyOED is developed to ``enable rapid development and benchmarking of OED methods with minimal coding effort and to maximize code reutilization.'' PyOED will be continuously expanded with a plethora of Bayesian inversion, DA, and OED methods as well as new scientific simulation models, observation error models, and observation operators. This paper provides a brief description of the PyOED layout and philosophy and provides a set of exemplary test cases and tutorials to demonstrate how the package can be utilized.Comment: 26 pages, 7 figures, 21 code snippet

Urban Metabolism and Quality of Life in Informal Areas

The 21st century is known as the century of urbanization. Numerous debates are currently taking place to definecities and what they should aspire to be. A number of terms have appeared in this arena,such as sustainable city, eco-city and green city to name a few. However, the main question remains how to measure the performance of a city in regards tothese aims. In addition, it is vital to note that major urbanization activities take part in cities of the developing world, where informalization is synonym to urbanization, thus necessitating a profound study of informal areas and their potential role in achieving sustainable cities. This paper studies how acity performs in terms of consuming and producing resources and how they flow through its various systems, described as urban metabolism. The paper particularly discusses how informal areas perform regarding their metabolism, focusing on water flow through these areas as a priority identified by the residents. Imbaba district, one of the largest informal areas in Cairo, is investigated as a case study to determine the actual quality of life of local residents and their ecological footprint and to providepractical insights. The whole process depends on a multidisciplinary participatory research where the citizens and local community based organization are the focal point. In addition, the process depends on open source data and data sharing as a way to empower local communities to identify their needs and issues and hence their appropriate interventions. This is conducted through questionnaires and interviews to identify what the current conditions and processes in informal areas provide for the residents. The paper concludes with identifying points of leakages in the resources flows and the possible interventions to improve the quality of life in the area while maintaining an efficient use of local resources and minimizing the impact of urbanization of the ecological footprint of cities. This will assist cities to become more resilient in the face of water scarcity, and provide a more vibrant life for its residents

Patient reported frequency of lupus flare: associations with foundation makeup and sunscreen use

Objective: To test the hypothesis that usage of foundation makeup (FM) and sunscreen lotion (SS), used individually or in combination, is associated with significant changes in the likelihood of lupus symptom exacerbation. Methods: Self-reported flare days (SRF) and use of FM and SS products, were retrospectively examined in 80 Caucasian Australian women with ACR classified SLE for a year. Negative binomial regression modelled SRF days (outcome) against independent FMSS variable and covariates: age; diagnosis years; outdoor hours; BMI; stress; immune therapy medication (ITM) use. Results: Statistically significant inverse associations between SRF days and FMSS use were found. Protective effects were statistically significant (p < 0.05) for combined FMSS exposure days (OR 0.998, CI 0.997 - 1.0) and FM alone (OR 0.603, CI 0.363 - 1.0). Significant associations consistent with increased SRF risk were seen in sub-analysis models for participants taking ITM: univariate model (OR 1.968, p = 0.03); multivariate model for FMSS (OR 2.11, CI 1.161 - 3.835); FM days (OR 1.855, CI 1.023 - 3.364). Results show SRF day reduction of 0.15% for each day of product exposure. Conclusion: Study results highlight protective effects of wearing FM with or without SS. This reduction in flare days ultimately has potential to improve quality of life in SLE patients

Vibrations analysis of ruptured pipe conveying pulsating fluid flow and supported by a magnetorheological damper

The study presented in this paper deals with the dynamics of a straight supported pipe conveying a harmonically pulsating incompressible fluid flow. It is assumed that the motion is planer and the pipe is normally horizontal, and there is a leakage from the pipe at a certain point and this leakage causes a repeated impulse forces acting on the pipe response. The pipe is fixed by two identical supports of three different types chosen for the sake of comparison. The first two supports are rigid, the second two are of conventional flexible type and are represented by springs and dampers while the third two supports are of Magnetorheological type and are represented by MR dampers. The variation of flow velocity and fluid pressure along the pipe is taken into consideration. The effect of leakage position and its amount on the dynamic performance and vibrations of the pipe is studied. The detection of leak location along the pipe is studied numerically using the spectrum analysis. The influence of supporting type and pulsating frequency on the pipe response is investigated. The effect of input voltage to the MR damper is also taken into consideration

Palm oil formulation as 34 % mayonnaise and evaluation of its biological efficacy against citrus mealybug, Planococcus citri under laboratory and field conditions

The use of environmentally friendly pesticides using palm oil derivatives as palm oil methyl ester (PME) carrier solvents has been reported. PME-based glyphosate isopropylamine nanoemulsion in the water against weeds has been the subject of numerous studies as well as palm oil methyl ester molluscicidal against golden apple snails. This study's major goal was to formulate palm oil in an appropriate formulation form and assess its insecticidal effectiveness against citrus mealybug, Planococcus citri. Palm oil was formulated as 34% oil in water emulsion (O/W) (mayonnaise). The new mayonnaise formulation successfully passed all physical and chemical testing requirements set out by pesticide organizations for (O/W) emulsions. Under laboratory conditions, it was biologically evaluated against nymphs and adults of the citrus mealybug, Planococcus citri, with serial concentrations. It had considerable insecticidal activity against all study stages, although the impact on nymphs was significantly greater than that on adults. This was evident from its LC50 values, which were 53.52 and 58.58 mg/ml for nymphs and adults, respectively. The citrus mealybug, Planococcus citri, in its adult, nymphs, and gravid stages, was tested using the new palm oil 34% mayonnaise formulation in the field. The highest mortality percentages were seen in the nymphs, followed by adults and then the gravid stage. After additional research, the newly developed palm oil formula might be employed to combat the citrus mealybug, Planococcus citri

Phenotypic and Genotypic Identification of Vancomycin Resistant Enterococci from Different Sources

Enterococci are reservoirs for transmission of the most clinically important antimicrobial resistances such as vancomycin resistance. Therefore, this work aimed to determine the occurrence of enterococci and their respective vancomycine resistance genes (vanA and vanB) from different sources. Two hundred and twenty-four samples from chickens, turkey, fish and human urine, as well as, two types of human food including milk (raw and milk from mastitic animals) and sausage were tested for isolation of Enterococcus species. The isolates were identified morphologically and biochemically using catalase test, sodium chloride tolerance and growth at pH 9.6 and 10- 45˚C. The vancomycin resistance profile of the isolates was verified by both disc diffusion and agar dilution methods. The genotypic enterococcal identification at both genus and species levels and their vancomycine resistance genes were also ascertained using PCR amplification of the respective genes for 28 isolates. Enterococci isolation rate was 70% of the examined samples with a higher percentage of vancomycine resistance (53.5%) and the minimum inhibitory concentrations (MICs) ranged from 16 to 512 µg/mL. Molecular identification of 28 enterococcal isolates revealed the dominance of E. faecalis (42.8%) and clarified a higher proportion of vanA (78.5%) and vanB (67.8%) genes. In conclusion, administration of the antimicrobials mainly vancomycin may be considered as a pronounced stress factor in the veterinary and human practices. In addition, VRE can act as a reservoir for vancomycin resistance

Effet du chrome niacinate sur la tolérance au glucose chez le rat wistar

Le chrome est un oligoélément essentiel à l’homéostasie du métabolisme glucidique qui pourrait également être impliqué dans l’étiologie de l’athérosclérose. Trois lots de rats Wistar males (n= 30, âgé de 2 mois) traités quotidiennement et pendant 28 jours. Les Contrôles ont reçu du sérum physiologique (1µL/g/j, ip), groupe II a reçu la dexaméthasone (DEX : 0,2 mg/kg/j, ip) et les rats du groupe III ont reçu le même régime que le groupe II avec une supplémentation en CrN à partir du 7 ème jour de l’expérimentation (CrN : 30 mg/kg/j, PO). A la fin de l’expérimentation, les rats sont sacrifiés et les paramètres biochimiques sont dosés. Les résultats montrent une augmentation du poids corporel des rats témoins par apport aux groupes traités par la DEX. La supplémentation en CrN n’a pas corrigé l’élévation de la glycémie et de l’insulinémie engendré par la DEX, de même l’ajout du CrN n’influe pas sur la variation des taux de la triglycéridémie et de la cholestérolémie entrainés par la DEX. Il est notamment observé que l’addition du CrN n’a pas d’effet notable sur la fonction hépatique et la fonction rénale.Mots-clés : chrome, diabète, glycémie, dexaméthasone.Effect of chromium niacinate on glucose tolerance at wistar rat Chromium is an essential trace element in the homeostasis of glucose metabolism could also be involved in the etiology of atherosclerosis. Three groups of male Wistar rats (n= 30, age 2 months) treated daily for 28 days. Controls received saline (1µl/g/day, ip), group II received dexamethasone (DEX: 0, 2 mg/kg/day, ip) and the rats of group III received the same treatment as the group II with supplementation CrN from the 7 day of the experiment (CrN: 30 mg/kg/day, PO). At the end of the experiment, the rats were sacrificed and biochemical parameters were measured. The results show an increase in the body weight of control rats contribution to the DEX treated groups. CrN supplementation did not correct the elevation of blood glucose and insulin caused by DEX. The addition of CrN does not affect the rate of change in triglycerides and cholesterol trained by DEX. It is particularly noted that the addition of CrN has no significant effect on liver and kidney functions.Keywords : chromium, diabetes, glucose, dexamethasone

The effects of calcite silicon-mediated particle film application on leaf temperature and grape composition of Merlot (Vitis vinifera L.) vines under different irrigation conditions

This study examined whether the application of calcite-silicon mediated particle film (CaPF) at veraison can mitigate a drought-induced increase in leaf temperature on grapevine, thus contributing to improved leaf functionality, yield and grape composition traits. A total of 48 five-year-old Merlot (Vitis vinifera L.) vines grafted onto SO4 were grown (in 20 L PVC pots) under Mediterranean conditions (Southern Italy). The vines were pruned to two spurs with two winter buds irrigated daily to 100 % field capacity, and fertilised weekly. At veraison and using a 2×2 factorial experimental design, the two main factors, thermoregulation and water, were imposed at two levels: spraying with a thermoregulation compound (CaPF) and no spraying (NS); irrigation (WW) and drought stress (D)). A group of 24 vines was subjected to a 15-day drought period by receiving, every day, 25 % (D) of the daily water consumption of WW vines. The other 24 vines continued to be fully irrigated on a daily basis (WW). Twelve vines per group were sprayed (WW+CaPF, D+CaPF) with calcite-silicon mediate (3 % V/V) at the beginning of drought imposition, the remaining 24 vines were not sprayed (WW-NS, D-NS). Soil water moisture and stem water potential values were monitored from 11.30 to 13:30 nearly every week, and other vegetative and reproductive parameters were also measured. During the experiment, air temperature peaked at ≈35 °C at midday, VPD at about 3.7 kPa and PAR reached ≈2000 µmol m-2 s–1. Results show that in CaPF sprayed vines, leaf-air temperature differences were lower than in unsprayed vines in both irrigated and drought stressed groups. WW+CaPF vines retained significantly more leaf area and showed the highest value of accumulated vine transpiration. Calcite-silicon mediated particle film could enhance the resilience of grapevine to adverse environmental conditions and may contribute to preserve terroir elements in highly reputed wine grape growing areas. The study showed that foliar application of calcite silicon-mediated processed particles films can be used in arid regions to mitigate leaf temperatures in grapevines