Melatonin inhibira lipidnu peroksidaciju u jetri štakora uzrokovanu benzenom

We studied the antioxidative role of melatonin against benzene toxicity in rat liver. The inhibition of mitochondrial and microsomal lipid peroxidation differed between 24-hour (single-dose), 15-day, and 30-day treatments. Inhibition of mitochondrial lipid peroxidation was the highest after the single dose of melatonin, whereas highest microsomal inhibition was recorded after 30 days of melatonin treatment. No signifi cant difference was recorded between 15-day and 30-day treatments. Cytochrome P4502E1 (CYP4502E1) activity declined after the single-dose and 15-day melatonin treatment in the benzenetreated group, but it rose again, though not signifi cantly after 30 days of treatment. Liver histopathology generally supported these fi ndings. Phenol concentration in the urine samples declined in melatonin and benzene-treated rats. Our results show that melatonin affects CYP4502E1, which is responsible for benzene metabolism. Inhibition of its metabolism correlated with lower lipid peroxidation. In conclusion, melatonin was found to be protective against lipid peroxidation induced by benzene.Istražena je antioksidacijska uloga melatonina u zaštiti protiv toksičnoga djelovanja benzena u jetri štakora. Utvrđeno je da kratkoročno odnosno dugoročnije liječenje štakora melatoninom u različitoj mjeri štiti štakore istodobno izložene benzenu. Inhibicija lipidne peroksidacije mitohondrija i mikrosoma bila je različita nakon 24 h, 15 dana, odnosno 30 dana liječenja melatoninom. Najveća inhibicija lipidne peroksidacije mitohondrija zamijećena je nakon primjene jednokratne doze melatonina, dok je najizraženija inhibicija u mikrosomima zamijećena nakon 30 dana liječenja melatoninom. Slična istraživanja pokazuju da razina glutationa (GSH) najviše raste nakon 24 h liječenja melatoninom. Nije zamijećena razlika između liječenja u trajanju od 15 odnosno 30 dana. U štakora koji su uz benzen istodobno primali i melatonin razine citokroma P4502E1 pale su nakon 24 h odnosno 15 dana izloženosti. U štakora koji su primali samo melatonin te su razine nakon 30 dana statistički neznačajno porasle u odnosu na skupinu izloženu samo benzenu. Histopatološka analiza jetre načelno je potvrdila ove nalaze. Koncentracije fenola u mokraći bile su niže u štakora koji su istodobno primali melatonin i benzen. Ovi rezultati pokazuju da melatonin utječe na citokrom P4502E1, koji je odgovoran za metabolizam benzena. Inhibira li se njegov metabolizam, smanjuje se lipidna peroksidacija. Zaključak je da melatonin štiti od lipidne peroksidacije uzrokovane benzenom

Effects of melatonin on both testicular regeneration and recovery of spermatogenesis in busulfan-treated rats

© 2022, Faculdade de Ciencias Farmaceuticas (Biblioteca). All rights reserved.Testicular damage is one of the most hazardous effects of chemotherapy as it is frequently associated with oligozoospermia and azoospermia. This study aimed at evaluating the protective effect of melatonin in a rat model of busulfan-induced testicular injury. Rats were divided into four groups: control, melatonin, busulfan, busulfan plus melatonin. After 15 days, the semen was collected from the epididymis and testes were assessed. Sperm removed from cauda epididymis and analyzed for sperm count and viability. Testis tissues were also removed, fixed in formalin and were embedded in paraffin. Sections of testis tissue were stained with hematoxylin-eosin for histological examination and prepared for TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay to detect apoptosis and PCNA (proliferating cell nuclear antigenassay) to detect proliferation cells. Serum and testes supernatants were separated to detect testosteron level and oxidative stress parameters. In histological examination, degenerative changes in seminiferous tubules were observed in the experimental groups. In biochemical examination, the total oxidant status (TOS) levels in Busulfan group were significantly higher than in the control group while the total antioxidant status (TAS) levels of all the groups were similar. In conclusion, the beneficial properties of melatonin treatment by its potent anti-oxidants may reduce adverse effects of chemotherapy in the reproductive system in a rodent system

Effects of melatonin and acetylsalicylic acid against hepatic oxidative stress after bile duct ligation in rat

The aim of this study was to assess the effect of melatonin and acetylsalicylic acid (ASA) on hepatic damage induced by bile duct ligation (BDL) Material and methods : Male Sprague-Dawley rats were subjected to either sham operation or common BDL before treatment with ASA, melatonin or vehicle. Hepatic superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) enzyme activities and reduced glutathione (GSH), malondialdehyde (MDA) and nitric oxide (NO) levels were evaluated. Results : Our results have indicated that BDL caused a significant increase in lipid peroxidation whereas a statistically insignificant decrease in GSH level and some of the antioxidant enzyme activities. Both MEL and ASA administrations, either separately or together, decreased MDA whereas co-administration of MEL with ASA increased GSH levels in BDL rats. Conclusions : CAT activity and MEL level decreased in the liver tissues of rats with BDL after administration of either melatonin alone or with ASA. However, melatonin and ASA administration increases liver tissue GSH levels in BDL ligated rats

The effect of prenatal exposure of a non-steroidal anti-inflammatory drug on the optic nerve of female rats: a stereological, histological, and electron microscopic study

Amaç: non-steroidal anti-enflamatuar ilaçlar (NSAID) hem de yan etki olabilir prenatal dönemde anne ve fetus takip yönetim . Sırasında verilirse gebelik, diklofenak sodyum (DS ) , bir NSAID ,gebelik sırasında verilir de etkileyebilir merkezi sinir sistemi (MSS) veya ilgili yapıların geliştirilmesi . Yöntem: Gebe sıçanlarda serum fizyolojik ( SG ) ve diklofenak gruplar , saf kontrol ( PG ) ayrıldı ( DG ) . 1 mg / kg DS ve 1 ml / kg tuzlu su içinde bir günlük doz, DG ile intraperitoneal olarak enjekte edilmiştir dönemin bir 15 gün için 5. gebelik günden itibaren sırasıyla ve SG gruplar ,PG grubu hiçbir tedavi uygulanmadı. Spontan Doğumdan sonra , dişi yavruların tüm elde edilmiştir grupları . Doğum sonrası hayat ,hayvanlar ( her grup için n = 6 ) 20. hafta perfüze edildikten sonra ve doğru optik sinirler rezeke edildi . Bölüm stereolojik ve tabi tutuldu histolojik analizi. Bulgular: ile PG , SG ve DG gruplar arasında anlamlı bir fark ( P40.05 ) vardı miyelin kalınlığı , akson kesit alanı , akson sayısal yoğunluğu , toplam bölümüne saygı optik sinir ve akson sayısının alanı . Sonuç: histolojik ve stereolojik sonuçları DS veya serum fizyolojik ile bu tedavi endikasyonu ile kadın sıçan optik sinir gelişimi ve myelinizasyon üretilen istenmeyen etkiler biçimbilimsel sayg